Polymorphism and bioinformatics analysis of MC4R gene in Macheng black goat

doi:10.14088/j.cnki.issn0439-8114.2021.21.031

Abstract

Abstract: MC4R （melanocortin receptor-4） is a G protein coupled receptor secreted by hypothalamus, which regulates feed intake and body weight of animals. In order to study the genetic specificity of Macheng black goat, MC4R gene polymorphism was detected by sequencing, and biological characteristics of MC4R were predicted by bioinformatics analysis. The results showed that there were 8 mutation sites of MC4R gene in Macheng black goat, namely C144T, T189A, A612G, C617A, C649G, C684A, T784A and C813A. According to the analysis of physicochemical properties, the amino acid sequence of MC4R contains a hydrophobic region and has seven transmembrane structures. The secondary structural elements encoding the protein are mainly the α Helix and irregular curl. The amino acid sequence alignment of MC4R among different species showed that the sequence similarity between Macheng black goat and sheep was 97.82%, and that between Macheng black goat and cattle was 94.50%, indicating that MC4R was a conserved protein and plays an important role in animal growth and development. In this study, eight MC4R gene mutation sites were obtained by sequencing, and bioinformatics analysis was carried out, which provided a theoretical basis for studying the molecular mechanism of MC4R and its natural mutants affecting goat growth and metabolism.

Key words: Macheng black goat, MC4R, mutation, polymorphism, bioinformatics

CLC Number:

S827
Q879

GUO Shuai, YANG Juan, LIU Shi-yu, YANG Feng-li, XIONG Qi, WANG Zhao-xiong. Polymorphism and bioinformatics analysis of MC4R gene in Macheng black goat[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(21): 140-145.

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