Effect of Gynostemma pentaphyllum polysaccharide on renal tissue structure of aging mice induced by D-galactose

doi:10.14088/j.cnki.issn0439-8114.2021.21.022

Abstract

Abstract: In order to investigate the effect of Gynostemma pentaphyllum polysaccharide （GPP） on the renal tissue structure of aging mice induced by D-galactose （D-gal）, paraffin section and HE staining were used to observe and analyze the glomerular diameter and renal capsule width in kidney. The results showed that in D-gal group, the glomeruli were significantly atrophied, the diameter of glomeruli became smaller, the lumen of renal tubules around the renal corpuscle was obvious, and the boundary of epithelial cells on the wall was unclear. GPP groups could significantly improve the structure of kidney damage, glomerular morphology tends to normal, the structure of renal tubules around the recovery. Statistical analysis showed that the glomerular diameter of GPP groups was significantly higher than that of D-gal group （P<0.05）, and there was no significant difference in glomerular diameter among the GPP groups. Compared with D-gal group, the width of renal capsule in 50 mg/（kg·d） group and 100 mg/（kg·d） group was significantly decreased （P<0.05）. It turned out that GPP could recover the kidney injury induced by D-gal, and the structural morphology tended to be normal; the diameter of glomerulus and the width of renal capsule could be improved, which indicated that GPP had a certain protective effect on the renal tissue structure of aging mice induced by D-gal.

Key words: Gynostemma pentaphyllum polysaccharide, kidney, aging, D-galactose, mice

CLC Number:

S859.4

LIU Xin-yong, LI Mu-lan, HUANG Jia-lin, WANG Xin-yu, TANG Xiao-chuan, WANG Xiao-li. Effect of Gynostemma pentaphyllum polysaccharide on renal tissue structure of aging mice induced by D-galactose[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(21): 94-96.

References

[1] FINKEL T, HOLBROOK N J.Oxidants, oxidative stress and the biology of ageing[J]. Nature, 2000, 408(9):239-247.
[2] PENG X, KONG B, YU H, et al.Protective effect of whey protein hydrolysates against oxidative stress in dgalactose-induced ageing rats[J]. International dairy journal, 2014, 34(1): 80-85.
[3] 魏芬芬, 王文娟, 张波. 枸杞多糖缓解小鼠体力疲劳研究[J]. 食品研究与开发, 2020, 41(6): 48-52.
[4] 龚天佐, 王世华. 当归多糖对D-半乳糖致小鼠肾脏亚急性损伤的保护作用及机制[J]. 当代医学, 2019, 25(29): 47-49.
[5] YU S S, YU J, DONG X D, et al.Structural characteristics and anti-tumor/-oxidant activity in vitro of an acidic polysaccharide from Gynostemma pentaphyllum[J]. International journal of biological macromolecules,2020,161(10):721-728.
[6] REN D Y,ZHAO Y,ZHENG Q,et al.Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells[J]. Food and function,2019, 10(4): 2186-2197.
[7] ANDERSON S,BRENNER B M,李金爱,等. 肾脏的衰老及其治疗[J]. 国外医学(老年医学分册),1989,10(3):114-115.
[8] 王佳贺. 前言—衰老相关机制的研究进展[J]. 实用老年医学, 2018, 32(12):1101-1102.
[9] 李典耕, 孙雪峰, 陈香美. 肾脏衰老机制的探讨[J]. 中华老年多器官疾病杂志, 2016, 15(4): 302-306.
[10] SARKAR D, FISHER P B.Molecular mechanisms of aging-associated inflammation[J]. Cancer letters,2006,236(1):13-23.
[11] COSTELLO W R, RYFF C D, COE C L.Aging and low-grade inflammation reduce renal function in middle-aged and older adults in Japan and the USA[J]. Age, 2015, 37(4): 75-84.
[12] 王珂欣, 高丽, 周玉枝,等. 衰老过程中炎症相关的信号传导通路研究进展[J]. 中国药理学与毒理学杂志, 2016, 30(10): 1108-1113.
[13] 刘晓梅, 陈尚. 枸杞多糖对衰老小鼠肾小球形态学指标的影响[J]. 中国老年学杂志, 2009, 29(18):2354-2355.
[14] 丁秋英. 金福菇多糖抗衰老作用研究[D]. 合肥:安徽大学,2016.
[15] 刘胜阳, 栗霄立. 灵芝多糖通过上调SIRT1的表达缓解肾脏衰老[J]. 临床和实验医学杂志, 2018, 17(6): 568-572.
[16] 蔡曦光, 许爱霞, 葛斌, 等. 菟丝子多糖抑制衰老小鼠模型中氧自由基域的作用[J]. 第三军医大学学报,2005,27(13): 1326-1328.
[17] 蒲秀瑛, 李海兵, 李晓玥, 等. 归芪多糖对衰老大鼠肾组织氧化损伤作用的研究[J]. 中医药学报, 2017, 45(2):15-18.
[18] 李丹丹, 刘佳佳, 刘军军, 等. 黄芪多糖联合二甲双胍对衰老2型糖尿病小鼠肾组织糖原代谢及超微结构的影响[J]. 时珍国医国药, 2019, 30(4):827-829.
[19] 傅紫琴, 蔡宝昌, 余宗亮, 等. 山茱萸炮制前后多糖成分对肾阴虚模型小鼠的抗衰老作用[J]. 中药新药与临床药理, 2007, 18(6):437-439.
[20] 金鑫. 绞股蓝多糖对小鼠腹腔巨噬细胞影响的研究[D]. 南宁:广西大学, 2018.
[21] WANG Z C, WANG Z, HUANG W H, et al.Antioxidant and anti-inflammatory activities of an anti-diabetic polysaccharide extracted from Gynostemma pentaphyllum herb[J]. International journal of biological macromolecules, 2020, 145(4):484-491.