HUBEI AGRICULTURAL SCIENCES ›› 2021, Vol. 60 ›› Issue (21): 73-75.doi: 10.14088/j.cnki.issn0439-8114.2021.21.017

• Horticulture & Local Products • Previous Articles     Next Articles

In vitro rapid propagation of axillary buds of Atractylodes macrocephala

LI Hong-ying1,2, CHEN Fei-fei1,2, YIN Hong-qing1,2, QIN Da-ji1,2, SUN Ju-zhi1,2, YANG Yong-kang1,2, XIANG Ji-qian1,2, LI Ya-jie1   

  1. 1. Enshi Tujia & Miao Autonomous Prefecture Academy of Agricultural Sciences,Enshi 445000,Hubei,China;
    2. Hubei Selenium Industry Technology Research Institute,Enshi 445000,Hubei,China
  • Received:2021-10-02 Online:2021-11-10 Published:2021-11-24

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Abstract: The axillary buds and shoot tips of Atractylodes Macrocephala Koidz were used as explants, and growth hormone （6-BA, NAA, etc.） with different concentrations were added to MS and 1/2 MS medium to induce differentiation and growth of the multiple shoots. Different concentrations of NAA and IBA were added to 1/4 MS medium for liquid rooting and strong seedling culture, and the cultured tube seedlings of Atractylodes macrocephala were transplanted. The results showed that the induction rate of tufted buds was 97.3% on the medium of 1/2 MS+1.5 mg/L 6-BA+0.4 mg/L NAA+0.3 mg/L IBA+0.6 mg/L GA3 under 18 h/d and illuminance 1 800 lx. The highest value-added multiple is 16; Medium：1/4 MS+0.4 mg/L NAA+0.2 mg/L IBA, illumination 18 h/d, illuminance 1 900 lx, rooting rate up to 98.9%, the average number of rooting 8.5 per shoot; The survival rate of seedlings was more than 95%. The survival rate of transplanted nursery was 94%.

Key words: Atractylodes macrocephala, axillary bud, liquid culture, cluster bud differentiation induction culture

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