Abstract: Taking Actinidia chinensis as sample, this article compared the effects of pollen preparation methods on pollen vitality. The pollen vitality was studied using TTC method, I₂-IK method and an in vitro method, and the optimical concerntration of sucrose, H₃BO₃ and CaCl₂ were selected by ‘3414’ test to find a proper method for measuring kiwifruit pollen vitality. The result show that mixing pollen with pollen shells is more conducive to germination of pollen tubes. TTC and I₂-IK are not suitable for the determination of kiwi pollen activity. The in vitro method can effectively reflect the true vitality of pollen. The optimal medium was 10% sucrose+0.1 mg/mL H₃BO₃+0.1 mg/mL CaCl₂ on pollen germination in vitro and the pollen germination ratio was up to77.9%.

Key words: kiwifruit（Actinidia chinensis）, pollen vitality, TTC, I₂-IK, in vitro germination