Abstract: The semi-lignified branches of Magnolia wufengensis were used as materials. The anatomical observation of rooting parts of cuttings was carried out by paraffin section method, and the activities of peroxidase （POD）, polyphenol oxidase （PPO） and indoleacetic acid oxidase （IAAO） in cutting branches were determined, and the process of adventitious root formation induced by 750 mg/L NAA was studied. The results showed that during the rooting process, the POD activity in the water treatment group （CK） and the growth regulator treatment group showed an increasing trend, and the content reached the highest at 35 d. The activity of PPO increased first and then decreased. The activity of PPO in the growth regulator treatment group and CK group increased from 0 to 14 d, and decreased sharply after 14 d. The activity of IAAO showed different trends. The activity of IAAO decreased first and then increased gradually in CK group from 0 to 7 d. The anatomical structure showed that there was no latent root primordium in the cuttings,cortex rooting type was developed from the inducted root primordium formed after cutting. Callus rooting type was originated from the vascular cambium between xylem and phloem. Adventitious roots produced by callus were specialized xylem formed by callus. Magnolia wufengensis belongs to the type of difficult to root. Low activity PPO, POD and high activity IAAO are beneficial to rooting, and additional growth regulators are needed to promote rooting. It is speculated that Magnolia wufengensis may be an induced rooting tree species, and adventitious roots can be generated by skin rooting and callus rooting.

Key words: Magnolia wufengensis, cuttings, anatomical structure, enzyme activity, physiology corresponding