Recombinant expression and functional study of dextran binding protein in Apostichopus japonicus

doi:10.14088/j.cnki.issn0439-8114.2021.19.029

Abstract

Abstract: β-1,3-glucan-binding protein （GBP） is a pattern recognition receptor （PRR）, which can recognize pathogenic microorganisms and activate the immune response. The GBP gene （AJ-GBP） was cloned from the sequenced transcriptome of Apostichopus japonicus by Polymerase Chain Reaction （PCR）. Nucleotide sequence analysis showed that the total length of the gene was 660 bp, and it could encode 219 amino acids without signal peptide. The theoretical molecular weight of AJ-GBP protein was 24.64 kDa, and the isoelectric point was 5.94. It belonged to the glycosyl hydrolases family 16, and there were glycosylation sites at positions 17 and 193. The results of protein activity test showed that the recombinant protein had agglutination, binding and inhibition effects on Gram-positive and Gram-negative bacteria. It has obvious binding activity to glucan. These results suggest that AJ-GBP may be involved in the innate immune response of Apostichopus japonicus. This study laid a theoretical foundation for understanding the mechanism of resistance to invasion of pathogenic microorganism and the actual production and utilization of AJ-GBP recombinant protein.

Key words: Apostichopus japonicus, β-1, 3-glucan-binding protein, recombinant expression, functional analysis

CLC Number:

Q819

JIA Chang-feng, CHEN Zhi-yu, LEI Yi-xuan, ZHANG Lei, KONG Ling-ming. Recombinant expression and functional study of dextran binding protein in Apostichopus japonicus[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(19): 135-140.

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