Infrared spectroscopy analysis of branches with different lignification degrees from tree peony

doi:10.14088/j.cnki.issn0439-8114.2021.19.015

Abstract

Abstract: Both FTIR （Fourier Transformation Infrared Spectroscopy） and Guassian multi-peak fitting were employed to investigate the spectrum properties and the differences of protein secondary structure in perennial branch with the highest lignification degree （branch 1）, annual branch with higher lignification degree （branch 2）, annual branch with lower lignification degree （branch 3） of four years old Paeonia ostii ‘Fengdanbai'. The result of FTIR showed that 12 characteristic peaks were observed. The peak intensity at 1 253, 1 385, 1 461 and 1 738 cm^-1 became greater, indicating that the perennial branch contained more lignin; a blue-shift was observed in branch 1 and annual branches from 1 043 cm^-1 to 1 053 cm^-1, which showed that cell wall polysaccharides of branch with high and low degree of lignification were glucomannan and araban, respectively; a red-shift was occurred in branch 1 and branch 3 from 1 426 cm^-1 to 1 410 cm^-1, which indicated that the pectin of cell wall of branch 3 was combined with metal cation through monodentate complex. The result of Guassian multi-peak fitting for the amide Ⅰ region （1 700~1 600 cm^-1） indicated that branches contained β-sheet+side chain + pectin, β-sheet, α-helix, loop+turn, and β-sheet+turn of secondary protein structures at different degrees of lignification. Branch 3 with low lignification contained more β-sheet associated with pectin, which might regulate the cell wall self-assembly; branch 1 with highest lignification contained low amounts of α-helix might compensate for reduced protein-water hydrogen bond caused by lignification.

Key words: Paeonia ostii ‘Fengdanbai', lignification, Fourier Transformation Infrared Spectroscopy （FTIR）, Guassian multi-peak fitting, protein secondary structure

CLC Number:

S567.1⁺5

LI Xiu-li, CHEN Zhen, JI Xiao-mei, DAI Zhi-gang, CHEN Fa-zhi, CHEN Zhi-wei, ZHAI Jing-hua. Infrared spectroscopy analysis of branches with different lignification degrees from tree peony[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(19): 75-80.

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