ARTP irradiation on pollen grain of Brassica napus and phenotype identification of their offsprings

doi:10.14088/j.cnki.issn0439-8114.2021.19.004

Abstract

Abstract: In order to obtain the mutant lines of high oil content rape, the ARTP mutation experiment of pollen grains of Brassica napus was carried out.The pollen of the restoring line 16C of Ogu CMS in Brassica napus was irradiated with ARTP mutation technology for 0, 20, 30 and 40 min respectively, and the mutated pollen was given to the sterile single plant 16CS naturally produced in the same population. Then traits of the progeny, such as seed number per pod, seed germination rate and energy and oil content were surveryed. The results showed that ARTP irradiation of Brassica napus pollen for more than 30 min could significantly reduce the number of single horned grains at the time of mutagenic pollen pollination and the germination potential and germination rate of M₀ generation seeds, improve the sterile plant rate of M₀ generation plants and the oil content of M₁ Generation seeds, and obtain the mutant line ARTP30-315 with an oil content of 52.61%. The preliminary results showed that it was effective to carry out rape pollen mutation by ARTP-P instrument, and the more suitable ARTP irradiation time was 30~40 min. A more suitable parameter system was explored for carrying out pollen mutation of Brassica napus by ARTP-P instrument in production, which provided a new method and idea for carrying out germplasm innovation of Brassica napus in the future.

Key words: Brassica napus L., Ogu-CMS restorer, pollen grain, ARTP mutation, male sterility, oil content

CLC Number:

S335

HE Xiao-ying, LU Wen-hu, SHI Wei-ce, WANG Wen-min, WANG Jing-qiao, CHEN Wei, ZHANG Guo-jian, ZHANG Jian-kun, SHU Zheng-qi, ZU Feng. ARTP irradiation on pollen grain of Brassica napus and phenotype identification of their offsprings[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(19): 19-22.

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