Optimization of extraction process of bile acid from bile paste by orthogonal design

doi:10.14088/j.cnki.issn0439-8114.2021.01.022

Abstract

Abstract: In order to optimize the extraction process of the bile acid in bile paste,the experiment used the L₉(3⁴) orthogonal test design,and the total bile acid content was used as an indicator,the amount of 1∶1 hydrochloric acid,the amount of NaOH and the purification time of acid-base purification process were selected to determine the optimal process conditions,and compared with the traditional ethanol purification process.The results showed that the best extraction process of bile acid in bile paste was 1∶1 hydrochloric acid dosage 4 mL,NaOH dosage 0.2 g,and purification time 12 h.The preferred extraction process has the advantages of low production cost,short purification time,small required space,low equipment requirements,simple operation,no pollution to the environment,no use of flammable and explosive organic solvents,good safety,and more in line with the actual needs of industrial production.

Key words: orthogonal design, bile paste, bile acid, extraction process, high performance liquid chromatography

CLC Number:

R286.0

JI Min, LIN Bai-liang, DENG Sheng-ju, PENG Jian-bo, DU Wei, YU Mei-ling, WANG Dong-ying, HE Jia-kang. Optimization of extraction process of bile acid from bile paste by orthogonal design[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(1): 104-107.

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