Screening, identification and the growth-promoting ability for mulberry growth-promoting bacteria

doi:10.14088/j.cnki.issn0439-8114.2021.01.017

Abstract

Abstract: In this study,a strain G2 with the ability to promote growth was isolated from mulberry rhizosphere soil.After morphological observation,physiological and biochemical characteristics and 16S rDNA sequencing comparison,the strain was identified as Pseudomonas schneideri.The effects of different pH value and culture time on the growth promoting ability of G2 strain were also studied.The results showed that the highest phosphorus dissolving ability of G2 was 17.96 mg/L at pH 7,and the secretion rate of IAA was faster before 48 h.Pot experiments showed that after inoculation with G2 solution diluted 100 times,the height,fresh weight,shoot dry weight and root dry weight of mulberry seedlings increased by 31.8%,38.5%,34.6% and 31.2%,respectively,compared with the control.The comprehensive analysis showed that G2 strain had strong growth promoting ability,which provided theoretical support for the development of mulberry microbial fertilizer.

Key words: pant growth-promoting bacteria, phosphorus-solubilizing, IAA, mulberry

CLC Number:

YANG Tong-yi, DU Qiu-xia, LIU Jing-fei, ZHAI De-li, LIU Yan-peng, ZHAO Wei-guo. Screening, identification and the growth-promoting ability for mulberry growth-promoting bacteria[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(1): 80-84.

References

[1] SHERATHIA D,DEY R,THOMAS M,et al.Biochemical and molecular characterization of DAPG-producing plant growth promoting rhizobacteria (PGPR) of groundnut (Arachis hypogaea L.)[J].Legume research,2016,39(4):614-622.
[2] BERENDSEN R L,CORNÉM J P,PETER A H M B.The rhizosphere microbiome and plant health[J].Trends in plant science,2012,17(8):478-486.
[3] 王岩,黄波,苏晓,等.植物根际促生细菌型生物肥料研究综述[J].山东林业科技,2013,43(3):92-95.
[4] 李蓉.杉木PGPR菌分离筛选及其微生物复合菌肥研制[D].长沙:中南林业科技大学,2012.
[5] WIJEKOON N,YAPA N.Assessment of plant growth promoting rhizobacteria (PGPR) on potential biodegradation of glyphosate in contaminated soil and aquifers[J].Groundwater for sustainable development,2018,7:465-469.
[6] 戴梅,宫象辉,丛蕾,等.PGPR制剂研发现状与发展趋势[J].山东科学,2006,19(6):45-48.
[7] 叶晶晶,曹宁宁,吴建梅,等.生物肥料的研究进展及在桑树上的应用[J].中国蚕业,2018,39(2):42-46.
[8] 刘泽平,王志刚,徐伟慧,等.水稻根际促生菌的筛选鉴定及促生能力分析[J].农业资源与环境学报,2018,35(2):119-125.
[9] 常慧萍,夏铁骑,付瑞敏,等.小麦根际解磷细菌的筛选鉴定及其促生效果[J].江苏农业科学,2018,46(14):270-273.
[10] XIAO A,LI Z,LI W,et al.The effect of plant growth-promoting rhizobacteria (PGPR) on arsenic accumulation and the growth of rice plants (Oryza sativa L.)[J].Chemosphere,2020,242:125-136.
[11] 高绘菊,付信芝,董法宝,等.桑树内生拮抗细菌枯草芽孢杆菌L144对植物生长及营养代谢的影响[J].蚕业科学,2010,36(2):214-220.
[12] 梁灏琳,王峥豪,莫芳姗,等.三种微生物菌剂在桑树上的应用效果初报[J].广东蚕业,2019,53(1):1-2.
[13] 罗海信.复合生物肥在桑树上的应用[J].四川蚕业,2015,43(1):32-33.
[14] 杨芬,巢波,杨万红,等.一株抗生素和重金属交叉抗性菌的筛选及抗性研究[J].环境工程学报,2017,11(2):1287-1292.
[15] YANG T,DING Y,ZHU Y,et al.Rhizosphere bacteria induced by aluminum-tolerant and aluminum-sensitive soybeans in acid soil[J].Plant soil &environment,2012,58(6):262-267.
[16] 杨统一,曲静怡,赵卫国,等.不同抗病性桑树品种根际土壤微生态环境特征分析[J].蚕业科学,2015,41(2):211-217.
[17] 姜睿玲,杨统一,唐玉斌,等.多环芳烃污染对桑园土壤微生物结构及种群多样性的影响[J].中国环境科学,2012,32(9):1655-1661.
[18] 刘丹丹,李敏,刘润进.我国植物根围促生细菌研究进展[J].生态学杂志,2016,35(3):815-824.
[19] ZHANG C S,KONG F Y.Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants[J].Applied soil ecology,2014,82(7):18-25.
[20] 宁爽.内生假单胞菌BTa14、Bar25促生抗病作用及机理的研究[D].山东烟台:烟台大学,2019.
[21] QESSAOUI R,BOUHARROUD R,FURZE J N,et al.Applications of new rhizobacteria pseudomonas isolates in agroecology via fundamental processes complementing plant growth[J].Scientific reports,2019,9(1):128-132.
[22] 李娜,洪坚平,谢英荷,等.一株高效溶磷细菌的筛选鉴定及溶磷效果的研究[J].山西农业大学学报(自然科学版),2014,34(6):526-530.
[23] 刘润进,李敏.一种AMF+PGPR组合菌剂及其制备方法和在分解残留有机磷农药中的用途[P].中国专利:2013100713428,2013-03-06.
[24] 邢丹,韩世玉,罗朝斌,等.接种丛枝菌根真菌对干旱条件下桑树生长及水分利用效率的影响[J].蚕业科学,2019,45(4):475-483.