Abstract: Taking the Angelica keiskei Koidzumi as test materials,through setting up different treatments,the optimal treatment methods and medium formulations of explants disinfection,axillary bud induction,proliferation culture,rooting culture and seedling transplanting were studied,and the tissue culture and rapid propagation technology system of the plant was established.The results showed that using 0.1% HgCl₂ disinfection for 10 min can achieved the ideal disinfection effect,the contamination rate was controlled at 13.3%,the germination rate was up to 83.3%,and the effective buds were up to 96.0%.The optimal culture medium for bud induction wasMS+0.80 mg/L 6-BA+0.10 mg/L NAA,and the buds began to germinate in about 4~6 days.The most suitable culture medium for subculture was MS+0.80 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L AD,and the coefficient of proliferation was 3.39.The rooting effect of single use of IBA or NAA is better than the combination of the two.When NAA concentration is 0.40 mg/L,the rooting rate reached 100% when cultured for 10 days,and the rooting number and average root length are better than other treatment combinations.The yellow-soil was selected as the transplanting substrate,and the seedlings with complete root system were transplanted.The survival rate was over 98% after 20 days.

Key words: Angelica keiskei Koidzumi, disinfection treatment, tissue-culture, transplanting