Determination of 8 residues of herbicide and mycotoxin in wheat by UPLC-MS/MS

doi:10.14088/j.cnki.issn0439-8114.2020.21.029

Abstract

Abstract: A method was developed in wheat by UPLC-MS/MS for determining 8 residues of herbicide and mycotoxin of isoproturon,MCPA,tribenuron methyl,bensulfuron methyl,fumonisin B₁, fumonisin B₂, sterigmatocystin,T-2. The wheat sample was extracted with acetonitrile-water-formic acid （85/14/1, V/V/V）,purified by appropriate amount of PestiCarb. The purified solution was concentrated, redissolved and centrifuged. 8 drugs were separated on ACQUITY UPLC BEH Shield RP18 column by gradient elution, then analyzed by ESI in positive and negative mode under MRM mode. The results showed that the linear range was from 2 to 100 μg/L with the correlation coefficients above 0.99 for all the 8 drugs. The average recoveries of the 8 drugs ranged from 76.4% to 114.0% and the RSD were 1.8%~9.2% in spiked wheat at three levels. The limits of quantification were 0.4~2.0 μg/kg. The method is suitable for the simultaneous determination 8 residues of herbicide and mycotoxins in wheat.

Key words: UPLC-MS/MS, wheat, herbicide, mycotoxin

CLC Number:

O657.7⁺1

ZHAO Jian, CHEN Guo, ZHANG Hao, WU Yin-liang. Determination of 8 residues of herbicide and mycotoxin in wheat by UPLC-MS/MS[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(21): 140-143.

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