Identification of the mutations in porcine MMP20 gene and its association analysis with reproduction traits

doi:10.14088/j.cnki.issn0439-8114.2020.21.022

Abstract

Abstract: In the present study two mutations （g.14334bpC>G and g.14369bpC>T） at intron 3 in porcine MMP20 gene were identified by Sanger sequencing. These two mutations were completely linked, and the association results showed that they significantly affected the reproduction traits （TNB, TNA, NAd21, BW and LW） in Large White pigs （P<0.05）. GG/CC genotype sows had the lowest litter size, followed by GC/CT genotype sows, while CC/TT genotype sows had the highest litter size.

Key words: pig, reproduction traits, MMP20 gene, mutation, association analysis

CLC Number:

S828
S813

WU Jun-jing, DONG Bin-ke, QIAO Mu, MEI Shu-qi, PENG Xian-wen. Identification of the mutations in porcine MMP20 gene and its association analysis with reproduction traits[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(21): 110-112.

References

[1] GAO Y, ZHANG L, XIANG L, et al.Transforming growth factor-β1 regulates expression of the matrix metalloproteinase 20 (Mmp20) gene through a mechanism involving the transcription factor, myocyte enhancer factor-2C, in ameloblast lineage cells[J]. European journal of oral sciences, 2014, 122(2): 114-120.
[2] ARNOLDO FILHO V A,CALIXTO M S,DEELEY K,et al. MMP20 rs1784418 protects certain populations against caries[J]. Caries research, 2017, 51(1): 46-51.
[3] CATERINA J J,SKOBE Z,SHI J,et al.Enamelysin (matrix metalloproteinase 20)-deficient mice display an amelogenesis imperfecta phenotype[J]. Journal of biological chemistry,2002,277(51): 49598-49604.
[4] 付廷刚,张静,韩婷婷. 基质金属蛋白酶-20的研究进展[J].解剖科学进展, 2011, 17(4):402-404.
[5] 赵倩,刘蜀. 高表达MMPMMP20可能是子宫内膜癌患者预后不良的潜在预测因子[J]. 南方医科大学学报, 2018, 38(10):1239-1244.
[6] ZHU X, SUN W.Association between matrix metalloproteinases polymorphisms and ovarian cancer risk: A meta-analysis and systematic review[J]. PLoS one, 2017, 12(9): e0185456.
[7] 王晗,董旭,葛常辉. 基质金属蛋白酶在生殖中的作用与调控[J]. 国外医学(计划生育分册),2002(3):141-144.
[8] 郭敏,黄凌霄,陈聪,等. 基质金属蛋白酶在子宫内膜异位症患者中的表达水平及在盆腔粘连中的作用[J]. 中国妇幼保健, 2020, 35(8):1390-1392.
[9] 高扬,朱艳. 子宫内膜异位症合并不孕患者血清中基质金属蛋白酶-9、基质金属蛋白酶抑制剂-1、血管内皮生长因子可溶性受体、胰岛素生长因子-1、单核细胞趋化蛋白-1表达水平及临床意义[J].陕西医学杂志, 2020, 49(4):462-465,501.
[10] 王卉菲,郭宇婧,王敏,等. 妊娠期高血压疾病胎盘浅着床的研究进展[J]. 国际妇产科学杂志, 2020, 47(2):165-168.
[11] 闫芳,王吉云,赵红敏,等. 孕激素及受体、基质金属蛋白酶表达与双胎早产的关系研究[J]. 中国性科学, 2019, 28(1):108-111.

[1]	ZHAO Hao-jian, ZHANG Huan-chun, LIU Ning, GUO Dui-tian, LI Shu-ping, ZHANG Li-li, ZOU Xiao-yu, YANG Jian-chao. Construction and operational strategy of order agricultural system based on blockchain technology——Taking Ningxiang pig in Ningxiang County, Changsha City as an example [J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(8): 202-206.
[2]	QIAO Tong-tong, ZHANG Yu, LI Zi-peng, QIAO Mu, QIN Zhen-yang, PENG Xian-wen, WU Jun-jing. Association analysis of NARS2 gene variation and carcass and meat quality traits in selenium black pig [J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 195-199.
[3]	LI Zheng, LIU Wen-chao, WANG Shi-yu. Research on operation mechanism and efficiency improvement of pig industry innovation ecosystem [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(9): 192-196.
[4]	WANG He-yun, SUN Tong, ZHOU Yi, JIN Hui, CHEN Jun-ge, YI Chun-long. The effects of light intensity and sedimental type on Chara braunii Gmel. in morphology, pigment pattern and sexual reproduction [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(8): 90-94.
[5]	WANG Yi-da, ZHOU Wang, CHEN Jin-jin, CAI Xiao-dong. Effects of different concentrations of NAA on cutting propagation of Caladium bicolosr under hydroponics [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(4): 81-84.
[6]	HE Wei-min, KUANG Shi-chang, LI Xiao-wen, WANG Chun, CUI Wei-tao, WANG Xi-liang, XIAO Yun-cai, LI Zi-li, ZHOU Zu-tao. Effects of microecological preparation of fermented Chinese medicine on the intestinal morphology, gene expression and flora of weaned piglets [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(16): 137-141.
[7]	ERKEJAN Hoyhazi, AJIGUL Sayit, MAMTIALI Mamtiyimin, APAR Ruzi. Study on temporal and spatial distribution characteristics of seasonal variation of air temperature in Xinjiang [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 25-33.
[8]	APAR Ruzi, ERKEJAN Hoyhazi, LI Qi, HUANG Jian, ZHANG Shan-qing. Analysis on temporal and spatial variation characteristics of temperature refinement in Xinjiang [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 34-41.
[9]	DING Feng, LI Pan, TANG Ya-li, PU Sheng-hai. Application of integrated water and fertilizer technology of drip irrigation under film mulching in the production of pigment pepper [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 129-132.
[10]	REN Qing-qing, ZHANG Shu-lan, ZHANG Hai-jun, LIU Quan-yu. Study on the variation of high temperature characteristic index of Ankang city in 1953—2020 [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(10): 33-38.
[11]	YIN Peng, WANG Zhao-hua. Situation and trend of pig industry in Shandong province under the epidemic situation of African swine fever and New Coronavirus pneumonia [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(1): 133-138.
[12]	XU Xiao-wan, XIA Bi-bo, WU Zhi-ming, TIAN Yong-hong. Surveying DNA epigenetic diversity in the foreign hot pepper germplasms [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(6): 76-80.
[13]	HUANG Zi-xuan, XIONG Yan-zhen, MA Hui-qin, CHENG Jian-feng. Research progress on the influence of pigmented rice germplasm and environmental factors on its color [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 5-10.
[14]	LU Xing-hua, LIU Pei-hao, ZHANG Hao. Research on meat pigeon food quality control system based on the perspective of production chain [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 145-148.
[15]	WANG Ying, LIU Xiao-yan, MIN Yong, ZHOU Rong-hua, RAO Ben. Study on the mutation of proteinase K and its properties [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(24): 105-107.