Identification and mutation breeding of a halophilic strain with high α-amylase-producing activity

doi:10.14088/j.cnki.issn0439-8114.2020.16.029

Abstract

Abstract: A halophilic strain NWU-8 that produced α-amylase was isolated and screened from the soil. Through morphological, physiological and biochemical and 16S rRNA gene sequence analysis, the strain NWU-8 was identified as Halorubrum sp., and the initial amylase activity was 32 U/mL. In order to improve the ability of the strain to produce α-amylase, the strain NWU-8 was mutated by He-Ne irradiation. After the mutation, the mutant NWU-8-14 produced alpha-amylase activity up to 138 U/mL, which was 331% higher than the wild strain. In addition, when the salt concentration was as high as 35%, its α-amylase activity still maintained 80 U/mL. NWU-8-14 was a kind of salt phages with high amylase production. This mutant strain had broad application prospects in food fermentation and environmental protection.

Key words: amylase, halophilic bacteria, mutation, enzyme activity

CLC Number:

TS201

ZHANG Wei, WU Xiao-wei, YAO Yan-hong, ZHANG Fen-fen, ZHOU Xiao-lun. Identification and mutation breeding of a halophilic strain with high α-amylase-producing activity[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(16): 130-133.

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