Abstract: Rapid multiplication and tissure culture were used to study the induction of buds and the proliferation and rejuvenation of cluster buds of Goodyera schlechtendaliana Rchh. f.. The results showed that with the increase of 6-BA concentration, the shoot proliferation rate increased, and a maximum of proliferation was obtained at 4.0 mg/L 6-BA, and multiplication of shoots could be achieved and controlled by combination of 6-BA 4.0 mg/L and NAA 0.2 mg/L. The rate of muhiplieation and growth could be improved in media supplemented 100.0 g/L coconut juice（CJ） and 0.5 g/L activated charcoal（AC）. Rooting of shoots were developed in 1/2 MS media with 0.5 mg/L IBA, 0.5 mg/L NAA and 0.5 g/LAC.

Key words: Goodyera schlechtendaliana Rchh. f., tissue culture, multiplication, rooting