Genetic character analysis of two wild mulberry varieties in Ankang based on chloroplast matK gene sequence

doi:10.14088/j.cnki.issn0439-8114.2020.15.030

Abstract

Abstract: The matK gene sequences of two wild mulberry that collected form Huangshitan and Baxian town （Ankang city, Shaanxi province） have been obtained by PCR. The length of amplified sequences is 1 715 bp while the ORF is 1 518 bp. Only one base difference when compared with each other. The genetic variations of 7 mulberry have been analyzed with the matK genes. The results showed that 6 variable sites were detected, which defined 3 haplotypes in Morus alba, and the haplotype diversity was 0.714. The nucleotide diversity was 0.001 32 and the average nucleotide difference was 2.000 respectively. As for the genetic distances, Baxian was closer to M. indica and M. multicaulis, while Huangshitan was closer to M. mongolica and M. cathayana. Cluster analysis demonstrated that the seven mulberries were grouped together, and the branch was shorter than other species, implied lower genetic diversity and fewer evolutionary events proceeded.

Key words: Morus alba L., phylogenetic analysis, genetic diversity, chloroplast, matK

CLC Number:

S888.2

WANG Rui-xian, CHU Qu, PENG Yun-wu, MENG Gang, LIANG Jia-jun, ZHOU Xiao-fan. Genetic character analysis of two wild mulberry varieties in Ankang based on chloroplast matK gene sequence[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(15): 151-154.

References

[1] 黄自然,杨军,吕雪娟. 桑树作为动物饲料的应用价值与研究进展[J]. 蚕业科学,2006(3):377-385.
[2] 范浩,庄愉. 桑叶药食同源开发应用研究进展[J]. 现代农业科技,2017(14):78-79, 83.
[3] 曾卫湘. 53份桑资源叶的药用品质评价[D]. 重庆:西南大学,2017.
[4] 中国农业科学院蚕业研究所. 中国桑树栽培学[M]. 上海:上海科学技术出版社, 1985.
[5] 张同武. 植物细胞器基因组测序,组装及比较基因组学研究[D]. 杭州:浙江大学,2012.
[6] HILU K W, LIANG H P.The matK gene: Sequence variation and application in plant systematics[J]. American journal of botany,1997,84(6):830-839.
[7] OZDILEK A, CENGEL B, KANDEMIR G, et al.Molecular phylogeny of relict-endemic liquidambar orientalis mill based on sequence diversity of the chloroplast-encoded matK gene[J]. Plant systematics and evolution,2012,298(2): 337-349.
[8] PLUNKETT G M,SOLTIS D E,SOLTIS P.Clarification of the relationship beteen apiaceae and araliaceae based on matK and rbcL sequence data[J]. American journal of botany,1997,84(4):565.
[9] SPECHT C D, KRESS W J, STEVENSON D W, et al.A molecular phylogeny of costaceae (Zingiberales)[J]. Molecular phylogenetics and evolution,2001,21(3):333-345.
[10] 刘宇婧,刘越,黄耀江,等. 植物DNA条形码技术的发展及应用[J]. 植物资源与环境学报,2011,20(1):74-82, 93.
[11] 滕艳芬,吴晓俊,徐红,等. 石斛及其常见混淆品的matK基因序列比较[J]. 中国药科大学学报,2002(4):18-21.
[12] 刘静,何涛,淳泽. 药用石斛的叶绿体matK基因序列分析及鉴别[J]. 药学学报,2009,44(9):1051-1055.
[13] 赵卫国,张志芳,潘一乐,等. 桑树trnL-trnF基因间隔区序列的特点及分析[J]. 蚕业科学,2002,28(2):83-86.
[14] 汪伟,王兴科,朱昱萍,等. 基于trnL内含子序列的桑属植物分子系统学初探[J]. 蚕业科学,2008,34(2):298-301.
[15] 王晖,高妍夏,高德满,等. 桑树matK基因的生物信息学分析[J]. 北方蚕业,2014,35(4):14-17.
[16] KONG W Q, YANG J H.The complete chloroplast genome sequence of Morus mongolica and a comparative analysis within the fabidae clade[J]. Curr genet, 2016, 62(1):165-72.
[17] KONG W Q, YANG J H.The complete chloroplast genome sequence of Morus cathayana and Morus multicaulis, and comparative analysis within genus Morus L[J]. Peerj, 2017, 5:e3037.