Effects of phosphate-solubilizing bacteria inoculated with different phosphorus levels on lettuce growth and soil phosphorus forms

doi:10.14088/j.cnki.issn0439-8114.2020.15.009

Abstract

Abstract: A potting experiment was conducted to evaluate the effects of the phosphate-solubilizing bacteria on lettuce growth and soil phosphorus forms at different growth stages of lettuce （Lactuca sativa var. ramosa Hort.） under four application levels of calcium-superphosphate. The results showed, under the condition of soil phosphorus deficiency, that the application of the phosphate-solubilizing bacteria could improve the leaf fresh weight, root fresh weight,leaf dry weight and root dry weight of lettuce, increased the net photosynthetic rate and transpiration rate, enhanced the phosphorus absorption capacity in lettuce plants, improved the nutritional quality and yield of lettuce, reduced the accumulation of whole phosphorus content in soil, and promoted the release of effective phosphorus and the accumulation of effective phosphorus in soil reservoir. In the case of the soil phosphorus abundance, the application of the phosphate-solubilizing bacteria was weaker influence on the growth of lettuce and soil phosphorus. When the amount of phosphate fertilizer in the soil was 0.375 g/kg, the phosphate-solubilizing bacteria promoting growth effect of lettuce and the soil phosphorus to release were the best. The application of the phosphate-solubilizing bacteria not only decrease the loss of phosphorus in agricultural land, mitigate the risk of phosphorus non-point source pollution, but also guarante the quality and quantity for farmers to maximize vegetable yield.

Key words: lettuce（Lactucas sativa L. var. ramosa Hort.）, phosphate-solubilizing bacteria, phosphorus level, growth, yield

CLC Number:

DU Lei, CHEN Gang, WANG Su-ping, HONG Juan, HUANG Xiang, ZHANG gui-you, LIAN Zhi-cheng, JIANG Li, YE Li-xia, ZHANG Li-hong. Effects of phosphate-solubilizing bacteria inoculated with different phosphorus levels on lettuce growth and soil phosphorus forms[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(15): 49-53.

References

[1] 任海红,刘学义,李贵全. 大豆耐低磷胁迫研究进展[J]. 分子植物育种,2008,6(2):316-322.
[2] 陈谦,张维新,赵海,等. 生物有机肥中几种功能微生物的研究及应用概况[J]. 应用与环境生物学报,2010,16(2):294-300.
[3] 乔志伟,洪坚平,谢英荷,等. 一株石灰性土壤强溶磷真菌的分离鉴定及溶磷特性[J]. 应用与环境生物学报,2013,19(5):873-877.
[4] 蒋欣梅,夏秀华,于锡宏,等. 微生物解磷菌肥对大棚茄子生长及土壤有效磷利用的影响[J]. 浙江大学学报(理学版),2012,39(6):685-688.
[5] 郝晶,洪坚平,刘冰,等. 不同解磷菌群对豌豆生长和产量影响的研究[J]. 作物杂志,2006,22(1):73-76.
[6] VASSILEV N,VASSILEVA M,NIKOLAEVA I.Simultaneous P-solubilizing and biocontrol activity of microorganisms: Potentials and future trends[J]. Applied microbiology & biotechnology, 2006, 71(2):137-144 .
[7] KHALID A,ARSHAD M,ZAHIR Z A.Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat[J]. Journal of applied microbiology,2004,96(3):473-480.
[8] HAMEEDA B, RUPELA O P, REDDY G, et al.Application of plant growth- promoting bacteria associated with composts and macrofauna for growth promotion of Pearl millet (Pennisetum glaucum L. )[J]. Biology and fertility of soils, 2006, 43(2):221-227.
[9] CHEN Z X,MA S W,LIU L L.Studies on phosphorus solubilizing activity of a strain of phosphobacteria isolated from chestnut type soil in China[J]. Bioresource technology,2008,99(14):6702-6707.
[10] REYES I,BERNIER L,ANTOUN H.Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum[J]. Microbial ecology, 2002, 44(1):39-48.
[11] 林启美,赵小蓉,孙炎鑫. 四种不同生态系统的土壤解磷菌细菌数量及种群分布[J]. 土壤与环境,2000,9(1):34-37.
[12] SUDHAKARA R M,SURENDER K,BABITA K,et al.Biosolubilization of poorly soluble rock phosphates by Aspergillus tubingensis and Aspergillus niger[J]. Bioresource technology,2002,84(2):187-189.
[13] 赵小蓉,林启美,孙焱鑫,等. 小麦根际与非根际解磷细菌的分布[J]. 华北农学报,2001,16(1):111-115.
[14] 杜雷,王素萍,陈钢,等. 一株高效解磷细菌的筛选鉴定及其溶磷能力的研究[J]. 中国土壤与肥料,2017(3):136-141.
[15] 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社,2000.
[16] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2001.
[17] SHEN J B, YUAN L X, ZHANG J l, et al. Phosphorus dynamics:From soil to plant[J]. Plant physiology,2011,156(3):997-1005.
[18] 陈梅梅,陈保冬,王新军,等. 不同磷水平土壤接种丛枝菌根真菌对植物生长和养分吸收的影响[J]. 生态学报,2009,29(4):1980-1986.
[19] 庞春花,杨世芳,张永清,等. 不同施磷水平下接种AM真菌对藜麦生长及产量构成因素的影响[J]. 作物杂志,2017(6):131-139.
[20] 于海彬,周芹,刘娜,等. 不同氮磷营养水平对甜菜叶片光合速率的影响[J]. 中国糖料,2001(3):19-21.
[21] 贺超,贺学礼,马丽,等. AM真菌和施磷量对黄芩光合特性和根系生长的影响[J]. 西北农业学报,2014,23(11):180-184.
[22] HE X L.Dynamics of arbuscular mycorrhizal fungi and glomalin in the rhizosphere of Artemisia ordosica Krasch. in Mu Us sandland, China[J]. Soil biology & biochemistry,2010,42(8):1313-1319.