HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (12): 175-178.doi: 10.14088/j.cnki.issn0439-8114.2020.12.039

• Biological Engineering • Previous Articles     Next Articles

Exploring the conditions for efficient electric transformation of Xanthmonas campestris pv. malvacearum （Xcm） V8

WANG Qing1, MENG Fan-qi1, ZHANG Yong-yue1, MIAO Wei-guo2, LIU Zhi-jian1   

  1. 1. Luohe Academy of Agricultural Sciences,Luohe 462300,Henan,China;
    2. Key Laboratory of Green Preventionand Control ofTropical Plant Diseases and Pests（Hainan University）, Ministry of Education,Haikou 570228,China
  • Received:2019-07-26 Published:2020-08-26

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Abstract: In order to obtain efficient electrical conversion conditions, experimentswere conducted to study the efficiency between optimal concentration for 5-AZA and factors playing roles in the efficiency of cotton keratinae [Xanthmonas campestris pv. malvacearum （Xcm）]V8 one by one. The results showed that V8 for preparing competent cells was domestricated by 5-AZA with a concentration of 100 μmol/L at the logarithmic metaphase, Whiah was used to acclimate V8 in the medium logarithm to prepare competent cells. When the guaranteed voltage was 2.1 kV, 7 μL plasmid as well as concentration of 50 μg/mL, recovery media SOC of 4 ℃ pre-cooling, culturing with time of four to five hours by oscillating, the efficiency could reach about 5 ×102 /μg DNA.

Key words: Xanthmonas campestris pv. malvacearum （Xcm）]V8, domesticating strains, condition of electrical conversion, the efficiency of electrical conversion

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