Abstract: The yield of passion fruit （Passiflora coerulea L. ） seed oil was used as the evaluation index, and single factor experiments were carried out on the basis of material-liquid ratio, time and temperature. Response surface methodology was used to optimize the extraction conditions of passion fruit seed oil, and the fatty acid composition of the extracted oil was determined and analyzed by gas chromatography-mass spectrometry （GC-MS）. The results showed that the optimum conditions were as follows, the ratio of material to liquid was 1∶30 （m∶V）, extraction time was 20 minutes, shaking temperature was 23 ℃, and the yield of oil in passion fruit seeds was 22.37%. There were 19 kinds of fatty acids in passion fruit seed oil, the relative amount of unsaturated fatty acids was 84.12%, the relative amount of saturated fatty was 15.88%. The main components were methyl passion （60.51%） and methyl trans-9-octadecanoate （22.05%）. The response surface methodology was used to optimize the extraction conditions to obtaina higher yield of passion seed oil. GC-MS analysis showed that the fatty acid composition of passion seed oil is not destroyed, which was a simple, efficient and non-destructive method.

Key words: passion fruit （Passiflora coerulea L.） seed oil, extract, response surface methodology, fatty acid, gas chromatography-mass spectrometry （GC-MS）