Isolation and identification of pathogen of pea fusarium wilt in Chongqing city

doi:10.14088/j.cnki.issn0439-8114.2020.06.016

Abstract

Abstract: The tissue isolation method was used to isolate and purify the pathogen of spot of fusarium wilt of peas in Chongqing city, and the isolates were identified by combining with the analysis of morphology and gene sequence. The pathogenicity of the isolates to the leaves and stems of isolated peas was determined by the method of inoculation without injury. The results showed that five strains were isolated from infected leaves. The colony was round, white flocculent in the early stage and yellowish brown to brown in the later stage; The homology of ITS sequence and G3PDH gene sequences of the tested strain reached 99% with that of Ascochyta pinodes; The results of pathogenicity determination showed that the strain had pathogenicity to both leaves and stems of pea. It indicated that Ascochyta pinodes was the cause of pea blight in Chongqing city.

Key words: pea, pea fusarium wilt, pathogens identified, Ascochyta pinodes

CLC Number:

S436.43

ZHANG Hui, MA Lian-jie, FENG Mu-ye, LU Wen-cai, LIAO Dun-xiu. Isolation and identification of pathogen of pea fusarium wilt in Chongqing city[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(6): 84-87.

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