HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (3): 144-146.doi: 10.14088/j.cnki.issn0439-8114.2020.03.032

• Biological Engincering • Previous Articles     Next Articles

Study on RNA difference of Xinjiang red meat apple extracted by different methods

TANG Lei1, TONG Pan-pan1,2, ZHANG Ya-ruo1,2, WANG Fang1, TANG Zhang-hu3, LI Wen-hui4, YUAN Zu-sheng5, WANG Jiang-bo1,2   

  1. 1.School of Plant Science,Tarim University, Alar 843300,Xinjiang,China;
    2.National Local Joint Engineering Laboratory of High efficiency and High quality cultivation and Deep processing Technology of characteristic Fruit trees in Southern Xinjiang,Alar 843300,Xinjiang,China;
    3.Xinjiang Academy of Agricultural Sciences Luntai National Fruit Tree Resource Garden,Luntai 841600,Xinjiang,China;
    4.Xinjiang production and Construction Corps fourth Division Forestry Management Station,Kedala 835099,Xinjiang,China;
    5.Agricultural Development Service Center of the 11th regiment of the first Division of the Xinjiang production and Construction regiment,Alar 843300,Xinjiang,China
  • Received:2019-10-13 Published:2020-05-11

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Abstract: The total RNA extraction and purification kit， Trnzol method and improved CTAB method were used to extract the total RNA， from the pulp， pericarp， petals and leaves of red meat apple Kizi Alma， and the results of yield， quality and integrity of the three methods were compared. The final results showed that the purity of RNA extracted by Trnzol method was low， the degradation phenomenon， and the pollution of DNA， protein， the yield of pulp and pericarp was not ideal， but the RNA extracted by improved CTAB method and kit method was rarely degraded. The 28 s rRNA and 18 s rRNA bands were clear， the OD260/280 of petals and leaves was between 1.8 and 2.5， the yield of RNA extracted by raw reagent kit method was high， the ratio of OD260/280 and OD260/230 was between 2.0 and 2.5， and the integrity of the bands was good. Compared with the three methods， the clarity， yield and quality of RNA bands extracted by modified CTAB method and TrNzol method were lower than those by raw reagent kit method.

Key words: Xinjiang red meat apple, RNA extraction, column plant RNA extraction and purification kit, Trnzol method, improved CTAB method

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