HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (9): 97-99.doi: 10.14088/j.cnki.issn0439-8114.2018.09.023

• Detection & Analysis • Previous Articles     Next Articles

Determination of Linolenate in Beefs-Gas Chromatographic Method

XI Bin, CHEN Xiao-wei, GAO Ya-qin, LI Wei-hong, GUO Tian-fen, DU Tian-qing, YANG Xiao-ling   

  1. Lanzhou Institute of Animal Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences/Quality Safety Risk Assessment of Animal Products (Lanzhou),Ministry of Agriculture/Quality Supervising,Inspection and Testing Center for Animal Fiber,Fur,Leather and Products,Ministry of Agriculture,Lanzhou 730050,China
  • Received:2017-12-27 Online:2018-05-10 Published:2019-12-23

Abstract: Linolenate was extracted with Isooctane from beefs,and methylified with KOH-CH3OH solution. Then content of methyl linolenate,which could be translated to the content of linolenate,was quantified with gas chromatographic. The retention time for methyl linolenate was 26.212 min. This method was appicable in a linear range of 0.01~10.00 mg/mL with the correlation coefficient at 0.999 95. The relative standard deviation(RSD) of intra-day and inter-day were 0.73%~1.08% and 0.75%~1.01%,which were less than 15%. The recovery rate was 83.3%~95.79%. The method gave high sensitivity,wide linearity,and specificity,which is suitable for determination of linolenate in beef.

Key words: gas chromatographic, beef, linolenate, methyl linolenate

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