Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.

doi:10.14088/j.cnki.issn0439-8114.2019.23.052

HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (23): 211-214.doi: 10.14088/j.cnki.issn0439-8114.2019.23.052

• Biological Engineering • Previous Articles Next Articles

Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.

ZHANG Hai-yang, FU Rao, LI Ru-xia, GU Yin-yu, LIANG Xiao-yan, SONG Yan-jing, LI Meng, WANG Xiang-yu, GUO Hong-en

Shandong Institute of Sericulture,Yantai 264002,Shandong,China

Received:2019-02-21 Online:2019-12-10 Published:2019-12-18

Abstract

Abstract: NHX1 gene was cloned from cultivated spinach （Spinacia oleracea L.） and named SpNHX1. Bioinformatics software were used to analyze the obtained gene nucleotide sequences and protein sequences encoded. The results showed that SpNHX1 belonged to the Vac subfamily, with a sequence length of 6 090 bp, an open reading frame of 1 662 bp, and 553 amino acid residues encoded. The bioinformatics analysis of SpNHX1 was carried out by using relevant software or online tools. The results showed that the molecular formula of its coding protein was C_{2 818}H_{4 363}N₆₉₇O₇₇₂S₂₅, which was a stable hydrophobic protein. Evolutionary tree analysis shows that SpNHX1 is closely related to AtNHX1 and AtNHX2 proteins of Arabidopsis thaliana, which belong to proteins located on vacuolar membranes. SpNHX1 protein is closely related to NHX proteins of dicotyledons.

Key words: Spinacia oleracea L., salt tolerance, SpNHX1, gene cloning, bioinformatic analysis

CLC Number:

S636.1

ZHANG Hai-yang, FU Rao, LI Ru-xia, GU Yin-yu, LIANG Xiao-yan, SONG Yan-jing, LI Meng, WANG Xiang-yu, GUO Hong-en. Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(23): 211-214.

References 10

[1]	WANG H,TANG X,SHAO C,et al.Molecular cloning and bioinformatics analysis of a new plasma membrane Na+/H+ antiporter gene from the halophyte Kosteletzkya virginica[J].Sci World J,2014,doi:10.1155/2014/141675.
[2]	LIU P,YANG G D,LI H,et al.Overexpression of NHX1s in transgenic Arabidopsis enhances photoprotection capacity in high salinity and drought conditions[J].Acta Physiol Plant,2010,32(1):81-90.
[3]	YANG Q,WU M,WANG P,et al.Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from alfalfa[J].DNA Seq,2005,16(5):352-357.
[4]	ZHAO J S,ZHI D Y,XUE Z Y,et al.Enhanced salt tolerance of transgenic progeny of tall fescue(Festuca rundinacea) expressing a vacuolar Na+/H+ antiporter gene from Arabidopsis[J].J Plant Physiol,2007,164(10):1377-1383.
[5]	CHEN L H,ZHANG B,XU Z Q.Salt tolerance conferred by overexpression of Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 in common buckwheat (Fagopyrum esculentum)[J].Transgenic Res,2008,17(1):121-132.
[6]	HAMADA A,SHONO M,XIA T,et al.Isolation and characterization of a Na+/H+ antiporter gene from the halophyte Atriplex gmelini[J].Plant Mol Biol,2001,46(1):35-42.
[7]	APSE M P,AHARON G S,SNEDDEN W S.Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis[J].Science,1999,285(5431):1256-1258.
[8]	FUKUDA A,NAKAMURA A,TANAKA Y.Molecular cloning and expression of the Na+/H+ exchanger gene in Oryza sativa[J].Biochim Biophys Acta,1999,1446(1-2):149-155.
[9]	GASTEIGER E,HOOGLAND C,GATTIKER A,et al.ProteinIdentification and analysis tools on the ExPASy server[A].WALKER J M. The proteomics protocols handbook[M].Totowa,NJ:Humana press,2005.571-607.
[10]	MARCHLER-BAUER A,BRYANT S H.CD-search:Proteindomain annotations on the fly[J].Nucleic Acids Res,2004,32(Web Server issue):W327-W331.

Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.

PDF

Knowledge

Abstract

Cite this article

share this article

References 10

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	ZHANG Yin-le, ZHAO Wei-jin, YU Liang, DANG Sai, ZHANG Yi, WANG Jian-lin. Effect of NaCl stress on seed germination and seedling growth of different highland barley cultivars [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(9): 19-24.
[2]	LU Shan, XIAO He-xia, MAO Cai-yun, YAN Xu-dong. Study on salt tolerance of 4 backbone maize inbred lines during germination period [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(20): 39-41.
[3]	YANG Guang-huan, NAN Ji-bin, YUAN Yan, GUO Su-ping, DAN Ba. Preliminary study on salt tolerance of Brassica napus L. seeds in Tibet area [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(16): 15-19.