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Table of Content

    25 December 2025, Volume 64 Issue 12
    New Quality Productivity in Agriculture
    Agricultural new quality productivity empowering rural revitalization: Theory and empirical evidence
    DENG Hao-tian, XIAO Feng
    2025, 64(12):  1-9.  doi:10.14088/j.cnki.issn0439-8114.2025.12.001
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    An evaluation index system for rural revitalization and agricultural new quality productivity was constructed, the entropy weight method was used to measure the levels of rural revitalization and agricultural new quality productivity in 30 provinces (municipalities and autonomous regions) in China from 2012 to 2023. Utilizing the two-way fixed effects model, mediation effect model, moderation effect model, and threshold effect model, the impact of agricultural new quality productivity on rural revitalization was empirically examined. The results showed that agricultural new quality productivity had a significant enabling effect on rural revitalization, and the impact of agricultural new quality productivity on rural revitalization varied in different regions. The agricultural new quality productivity could promote rural revitalization by promoting agricultural technological progress. Rural population aging could attenuate the promoting effect of agricultural new quality productivity on rural revitalization, whereas land scale operation strengthened this positive relationship. The impact of agricultural new quality productivity on rural revitalization exhibited a nonlinear characteristic of diminishing marginal effects. This study could provide reference for relevant departments to formulate rural revitalization policies in the process of developing agricultural new quality productivity.
    The impact mechanism of new agricultural productive forces on agricultural carbon emission efficiency
    LI Xue, LI Lian-jun
    2025, 64(12):  10-18.  doi:10.14088/j.cnki.issn0439-8114.2025.12.002
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    Based on the undesirable-output super-efficiency SBM model and the GML index, the spatiotemporal patterns and driving mechanisms of agricultural carbon emission efficiency across Chinese provinces from 2011 to 2023 were measured. The results showed that the static efficiency measured by the super-efficiency SBM model exhibited significant regional differentiation: Regions with ecological advantages, such as Tibet and Xinjiang, achieved the highest efficiency, while Gansu and Anhui showed relatively low efficiency due to resource constraints or excessive agricultural input; the dynamic efficiency reflected by the GML index indicated that technologically active regions were primarily driven by technological progress, whereas some resource-rich regions experienced a decline in technical efficiency, revealing multifaceted constraints on technology implementation; spatial distributions of total factor productivity in 2012, 2016, 2020, and 2023 further demonstrated significant fluctuations in agricultural carbon emission efficiency in certain regions, resulting from their failure to adapt to technological upgrades under natural environment and industrial restructuring; quantile regression results indicated that the lagging labor structure hindered the development of high-efficiency regions, agricultural mechanization faced high-energy-consumption bottlenecks in medium-efficiency and high-efficiency regions, land transfer rate exerted multi-dimensional inhibitory effects on carbon emission efficiency, whereas the multiple cropping index played a significantly positive role at high quantiles.
    Theoretical mechanism and empirical test of new quality productive forces empowering digital rural construction
    LI Jie, GUAN Fan-hua, LI Bao-dong, LYU Wen-jin
    2025, 64(12):  19-27.  doi:10.14088/j.cnki.issn0439-8114.2025.12.003
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    Based on panel data from 31 provinces in China from 2011 to 2022, fixed-effects models, mediating effect models, and threshold effect models were employed to systematically examine the impact of new quality productive forces on digital rural construction and its underlying mechanism. The results indicated that new quality productive forces had a significant positive effect on digital rural construction. Agricultural sci-tech innovation was found to play a partial mediating role in this process, and this impact exhibited a dual threshold effect based on the level of agricultural sci-tech innovation. As the level of agricultural sci-tech innovation increased, the promoting effect of new quality productive forces on digital rural construction showed a characteristic of "diminishing marginal effects." From the perspective of regional heterogeneity, the promoting effect followed the pattern of central region > western region > eastern region. When regions were divided by the level of digital rural construction, the promoting effect was significantly greater in high-level regions than in low-level regions. Based on these findings, it was recommended to continuously enhance the development of new quality productive forces, strengthen the driving role of agricultural sci-tech innovation, promote technology empowerment according to local conditions, stimulate the vitality of digital rural construction, and facilitate its digital transformation and high-quality development.
    Breeding & Cultivation
    Breeding and application of Huyou 71, a dual-purpose rice variety for late-season and ratooning cultivation
    DU Xue-shu, YANG Da-bing, WAN Bing-liang, YOU Ai-qing, XIA Ming-yuan, HU Liang, DONG Hua-lin, LI Jun, LI Jin-bo
    2025, 64(12):  28-32.  doi:10.14088/j.cnki.issn0439-8114.2025.12.004
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    Huyou 71 was a new three-line hybrid rice variety developed using Xiadao No.1 as the male parent and Huhan 7A as the female parent. It was approved by the Hubei Provincial Crop Variety Approval Committee in 2024. This variety demonstrated high yield potential, excellent grain quality, strong resistance to rice blast, and robust ratooning ability. It has also passed the joint regional trial for late indica rice and ratooning rice in Hubei Province. In view of its characteristics, it was considered that the cultivation of this variety should be sown in time according to the needs, and the suitable height of stubble should be selected for the cultivation of ratooning rice. At the same time, nitrogen should be controlled and potassium should be increased to improve lodging resistance.
    Coupling effect of planting density and fertilization method on potato yield formation and economic benefits
    ZHANG Cui-cui, WANG Yan-ping, YI Chun-yan, CHEN Guan-tao, CHEN Qing-dong, WANG Yang-mei, WANG Xie, LUO Yong-xia
    2025, 64(12):  33-37.  doi:10.14088/j.cnki.issn0439-8114.2025.12.005
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    Six potato (Solanum tuberosum L.) varieties, including Feiwuruita, Gaoyuan No.2, Gaoyuan No.8, Qingshu No.9 original seed, Heimeiren (purple potato) and Wuyangyu, were used as experimental materials, and self-reserved seeds of Qingshu No.9 was used as control. Two planting densities, high density (57 000 plants/hm; 2) and low density (40 500 plants/hm; 2), were set to study their effects on potato yield and economic benefits. Additionally, using Wuyangyu as the material, six fertilization treatments were set up with two planting densities, and the effects of fertilization and planting density on the yield of Wuyangyu were analyzed. The results showed that, except for Heimeiren(purple potato), the per-plant yield under low-density planting was significantly higher than that under high-density planting. The effect of planting density on yield per hectare varied by variety. Under high-density planting, some varieties exhibited higher yields per hectare than under low-density planting, with Qingshu 9 original seed, Qingshu 9 self-reserved seed, and Heimeiren showing highly significant yield increases under high density. Wuyangyu achieved the highest yield of 37 976.12 kg/hm; 2 when planted at high density with the application of organic fertilizer + compound fertilizer + 2.5 g/plant potassium sulfate. Among all varieties, Qingshu 9 original seed under high-density planting achieved the highest yield of 62 094.45 kg/hm; 2. In terms of economic benefits, Heimeiren (purple potato) performed the best, with net profits of 47 303.40 yuan/hm; 2 and 59 134.20 yuan/hm; 2 under low and high densities, respectively, followed by Wuyangyu.
    Artificial emasculation and hybridization between Yunqiao No.1 and Mikuqiao, and agronomic trait analysis of their hybrid offspring
    YIN Gui-fang, WANG Yan-qing, SUN Dao-wang, LONG Wen-jie, LU Wen-jie, WANG Li-hua
    2025, 64(12):  38-41.  doi:10.14088/j.cnki.issn0439-8114.2025.12.006
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    To provide references for improving the hybridization success rate of tartary buckwheat [Fagopyrum tataricum (L.)Gaertn.]and breeding large-grain, easy-to-thresh, high-yield tartary buckwheat varieties, the artificial emasculation hybridization method was applied. Using Yunqiao No.1 (thick-shelled) as the female parent and Mikuqiao (thin-shelled) as the male parent, hybrid offspring were obtained. Agronomic traits of the parents and the F2 generation, including plant height, number of main stem nodes, number of primary branches, grain weight per plant, grain number per plant, thousand-grain weight, and shell type, were compared and analyzed. The results indicated that selecting female parent flowers with mature stigmas and anthers that have not yet dehisced was particularly crucial during the hybridization process. There were no significant differences in the mean values of traits between the thick-shelled F2 population and Yunqiao No.1. Compared to Mikuqiao, the thin-shelled F2 population showed significantly lower mean values for plant height and number of primary branches, while the mean thousand-grain weight was significantly higher. The coefficient of variations for grain weight per plant, and grain number per plant was the large among the traits in the F2 generation. The maximum grain number per plant reached 2 577 grains for the thick-shelled type and 3 817 grains for the thin-shelled type, far exceeding the 986 grains of the female parent and the 2 035 grains of the male parent. Similarly, the grain weight per plant was also the highest, with the thick-shelled type at 49.60 g and the thin-shelled type at 45.65 g, significantly exceeding the female parent’s 18.69 g and the male parent’s 27.13 g. Through hybridization, the plant traits of both thick-shelled and thin-shelled types were improved. The maximum values for grain number per plant, and grain weight per plant were significantly increased in the thick-shelled F2 plants, while the thin-shelled F2 plants showed a significant increase in thousand-grain weight and a significant decrease in plant height and number of primary branches.
    Screening experiment of rice varieties under the shrimp-rice co-cropping model in Qianjiang City
    YU Qi-zhi, HU Jian-lin, XUE Lian, ZHENG Xing-fei, DONG Hua-lin, WANG Ben-fu, WANG Hong-bo, PENG Xuan-guo, ZHONG Yu-ping, WU Wen-hui, XU De-ze
    2025, 64(12):  42-46.  doi:10.14088/j.cnki.issn0439-8114.2025.12.007
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    To advance the high-quality development of shrimp-rice industry in Qianjiang City, Hubei Province, 19 new rice varieties independently bred by the Institute of Food Crops, Hubei Academy of Agricultural Sciences were collected, and a systematic screening experiment under the local shrimp-rice co-cropping regime was conducted. After comprehensive evaluation, two high-yield and high-quality shrimp rice varieties, Yangxianyou 77 and E Liangyou 2300, were screened out from the late-maturing varieties. Among them, the yield of Yangxianyou 77 reached 12 483.30 kg/hm; 2, which was 56.54% higher than that of the control Huanghuazhan, and the rice quality reached the third grade of the ministry standard under extreme high temperature conditions. The yield of E Liangyou 2300 was 10 348.50 kg/hm; 2, which was 29.77% higher than that of the control Huanghuazhan, and the rice quality also reached the third grade of the ministry standard under extreme high temperature conditions. A high-yield and high-quality shrimp rice variety Yaliangyou Meixiangxinzhan was screened out from early maturing varieties. The yield was 11 056.50 kg/hm; 2, which was 38.64% higher than that of the control Huanghuazhan. The rice quality reached the third grade of the ministry standard under extreme high temperature conditions. In addition, the yield of E Nongxiang 11S/HD227 was 11 060.25 kg/hm; 2, which was 38.69% higher than that of Huanghuazhan.
    Resource & Environment
    Effects of different substitution ratios of organic fertilizer with equivalent nitrogen for chemical fertilizer on wheat yield and nitrogen fertilizer utilization efficiency
    CHEN Xiao-ling, XING Jin-cheng, FANG Yuan-jiang, LIU Chong, ZHU Xiao-mei, HE Ting-ting, DONG Jing, HE Su-nan, SUN Guo-li
    2025, 64(12):  47-51.  doi:10.14088/j.cnki.issn0439-8114.2025.12.008
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    To improve wheat yield, fertilizer utilization efficiency, and economic benefits, a field experiment was conducted with seven treatments, including no fertilizer (CK), conventional fertilization (CF), and 10%, 20%, 30%, 40%, and 100% substitution of chemical fertilizer with organic fertilizer with equivalent nitrogen (10% OM to 100% OM). The effects of different substitution ratios on wheat yield and its components, nitrogen fertilizer utilization efficiency, and soil physicochemical properties were systematically evaluated. The results showed that partial substitution treatments increased spike number, grains per spike, 1000-grain weight, and wheat yield. The highest wheat grain yield of 570.3 kg/667 m; 2 was achieved under the 20% OM treatment. The total nitrogen, total phosphorus, and total potassium contents in wheat grain under the 10% OM to 40% OM treatments were higher than those under the CF treatment. The partial factor productivity of nitrogen fertilizer, agronomic efficiency of nitrogen fertilizer, and nitrogen harvest index reached their highest values under the 20% OM treatment, while the apparent nitrogen fertilizer utilization efficiency was the highest under the 30% OM treatment. Compared with the CF treatment, the application of organic fertilizer generally increased the contents of soil organic matter, total nitrogen, available phosphorus, and available potassium, with all these indicators reaching their peak under the 100% OM treatment. Soil organic matter and total nitrogen significantly promoted wheat grain yield and its components. Considering wheat yield, nitrogen fertilizer utilization efficiency, and soil physicochemical properties comprehensively, a substitution ratio of 20% to 30% for organic fertilizer with equivalent nitrogen was found to be appropriate, which contributed to wheat yield increase, improved nitrogen fertilizer utilization efficiency, and enhanced soil quality.
    Effects of continuous cropping on soil physicochemical properties, nutrients, and heavy metal content of Pinellia ternate soil from Qianjiang
    ZHAN Ya-bin, PAN Qiao-yun, LIU Bo, PAN Wei-ming, YANG Ma, LI Hui, SHI Qian-man, HE Zhi-gang
    2025, 64(12):  52-55.  doi:10.14088/j.cnki.issn0439-8114.2025.12.009
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    In order to systematically evaluate the soil condition of long-term continuous cropping (for seven consecutive years) bases of Pinellia ternata in Qianjiang City, 49 soil samples were randomly collected from different cultivation greenhouses to determine the physicochemical properties, nutrient contents, and heavy metal concentrations. The results indicated that the soil was generally alkaline(average pH 7.56), with a moderate organic matter content(17.67 g/kg), and exhibited slight salinization(average EC 0.76 mS/cm). The contents of total nitrogen and total potassium were moderate, while total phosphorus, alkali-hydrolyzable nitrogen, available phosphorus, and available potassium were all at high levels. Available boron was high; available iron, copper, and zinc were moderate; while available manganese and molybdenum were relatively low. The contents of total cadmium, chromium, lead, nickel, mercury, and arsenic were all below safety thresholds. However, the detected total cadmium in some samples reached 0.3 mg/kg, approaching the risk limit. Based on these findings, it was recommended that subsequent cultivation practices adopted integrated measures such as salinity control, optimization of the fertilization structure (reducing chemical fertilizers and increasing organic fertilizers), and strict regulation of exogenous cadmium input, in order to maintain soil health and guarantee the quality of Pinellia ternata.
    Effects of topdressing organic-inorganic compound fertilizer on the yield and quality of three oil-vegetable double usage type rape varieties
    CHU Qian-mei, YIN Yu-feng, XIE Xiong-ze, XIE Jie, YU Xiao-hong, WANG Dun, FENG Peng
    2025, 64(12):  56-60.  doi:10.14088/j.cnki.issn0439-8114.2025.12.010
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    In order to explore the effects of topdressing organic-inorganic compound fertilizer on the yield and quality of flowering Chinese cabbage and rapeseed, the main rape varieties Huayouza 62 (C1), Zhongyouza 501 (C2) and Zhongyouza 19 (C3) in Hubei Province were used as the research objects, and three topdressing treatments of organic-inorganic compound fertilizer [0 (Z1, no topdressing), 225 kg/hm; 2 (Z2), 450 kg/hm; 2 (Z3)] were set up. The effects of topdressing organic-inorganic compound fertilizer on the yield and quality of flowering Chinese cabbage and rapeseed were studied. The results showed that the yield of flowering Chinese cabbage and rapeseed with topdressing organic-inorganic compound fertilizer was significantly higher than that without topdressing. Among them, except for the yield of flowering Chinese cabbage of Zhongyouza 501 and Zhongyouza 19, there was no significant difference in the yield of flowering Chinese cabbage and rapeseed between the topdressing amount of 450 kg/hm; 2 and the topdressing amount of 225 kg/hm; 2. Compared with no topdressing, the soluble sugar content, sucrose content and vitamin C content of flowering Chinese cabbage increased after topdressing organic-inorganic compound fertilizer. With the increase of topdressing amount, the contents of vitamin E and amino acids in flowering Chinese cabbage increased first and then decreased. After topdressing organic-inorganic compound fertilizer, the protein content and erucic acid content in rapeseed decreased, and the oil content, glucosinolate content and oleic acid content increased. Considering the yield and quality indexes of flowering Chinese cabbage and rapeseed, the optimum topdressing amount of organic-inorganic compound fertilizer for three kinds of oil-vegetable double usage type rape was 225 kg/hm; 2.
    Effects of wheat rotation on soil metabolites in continuous cropping greenhouses of watermelon
    YI Li-cong, ZENG Fen, WU Na, ZHOU Wei, WANG Yun-qiang, DAI Zhao-yi
    2025, 64(12):  61-65.  doi:10.14088/j.cnki.issn0439-8114.2025.12.011
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    The effects of wheat rotation on the species and abundance of soil metabolites in continuous cropping greenhouse of watermelon (Citrullus lanatus) were investigated by wide-targeted liquid chromatography-mass spectrometry. The results indicated that there were significant differences in both the composition and abundance of metabolites between the winter fallow soil (CK) and the wheat-rotated soil (WT). A total of 1 405 metabolites were detected in the two soil groups, which were mainly concentrated in lipid and lipid-like molecules, organic acids and their derivatives, organic heterocyclic compounds, benzene derivatives, etc. A total of 228 differential metabolites were identified, including 41 up-regulated metabolites and 187 down-regulated metabolites. Compared with the CK, the abundance of compounds associated with anti-pathogenic microorganism in the WT group was significantly higher, such as roridin L, reticulinylium, and smilagenin. Conversely, the abundance of compounds linked to continuous cropping obstacles, such as indoxyl sulfate, bisphenol, behenic acid, and 9,10-epoxy stearic acid, was markedly reduced. KEGG enrichment analysis revealed that the pathway of cutin, suberin, and wax biosynthesis was significantly enriched. It could be seen that wheat rotation could improve the types and abundance of metabolites in watermelon continuous cropping greenhouse soil.
    Synergistic enhancement effects of controlled-release fertilizer, organic fertilizer, and microbial inoculant on physiological metabolism, yield, and quality of Amorphophallus konjac variety Anmo 128
    HE Mao-chang, LIU Bin, SUN Ge-li-sha, ZHOU Zhi-hao, LIU Chen-yu, HE Fei, WANG Gang
    2025, 64(12):  66-71.  doi:10.14088/j.cnki.issn0439-8114.2025.12.012
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    To investigate the effects of the combined application of controlled-release fertilizer, organic fertilizer, and microbial inoculant on growth of Amorphophalms konjac, a study was conducted using the common local practice of applying farm compound fertilizer alone as a control. The comprehensive effects of their combination on the physiology, yield, quality of the Amorphophalms konjac variety Anmo 128, and the soil environment were studied. The results showed that compared with the control, the activities of superoxide dismutase and peroxidase, the contents of soluble protein, soluble sugar and free proline in the leaves of Amorphophalms konjac treated with controlled release fertilizer+organic fertilizer+microbial agent (NOM) were significantly increased, while the contents of H2O2 and malonaldehyde were significantly decreased. The combined fertilization treatments significantly increased tuber yield and improved bulb quality of Amorphophalms konjac. Specifically, the NOM treatment significantly increased the total yield of corms and stolons and the number of disease-free and pest-free corms by 35.9% and 40.5%, respectively, compared to the control. The cellulose content, glucomannan content, and viscosity also significantly increased by 75.6%, 22.3%, and 16.5%, respectively. Furthermore, the treatment of NOM significantly increased the available potassium and organic matter content in the Amorphophalms konjac rhizosphere soil by 5.5% and 13.4%, respectively, and significantly enhanced the activities of soil catalase, urease, and dehydrogenase by 276.7%, 121.4%, and 72.0%, respectively, compared to the control. The comprehensive evaluation showed that the treatment of NOM had the highest score, indicating that it had the best synergistic effect on the yield and quality of Amorphophalms konjac.
    Effects of rapeseed cake fertilizer replacing iso-nitrogen fertilizer on rhizosphere soil fertility and yield and quality of flue-cured tobacco
    ZHANG Hai-wei, WANG Nian-lei, DING De-sheng, ZHANG Xiong-feng, XIA Hao, LI Zhuo, LI Hui-lan, ZHAO Zun-kang
    2025, 64(12):  72-77.  doi:10.14088/j.cnki.issn0439-8114.2025.12.013
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    In order to explore the suitable application mode of organic fertilizer and chemical fertilizer in Jiangxi tobacco-growing area, randomized block experiment design was introduced to study the effects of rapeseed cake replacing iso-nitrogen fertilizer on soil physico-chemical properties, leaf growth, yield and quality of tobacco at two sites in Ruijin City and Xiajiang County. The results showed that the substitution of chemical fertilizer nitrogen with rapeseed cake fertilizer at proportions of 15% to 35% could improve the rhizosphere soil nutrients and microbial environment of flue-cured tobacco in different extents. The contents of soil organic matter, alkali-hydrolyzed nitrogen, available phosphorus and available potassium at the two test sites were 3.18%-10.79%, 2.56%-23.39%, 7.51%-33.86%, and 5.12%-25.12% higher than those of the single application of chemical fertilizer, respectively. The number of soil bacteria increased by 13.62%-46.16%, the number of actinomyces increased by 12.64%-45.30%, and the number of fungi decreased by 8.73%-23.12%. Compared with single fertilizer application, the yield, output value, aroma substance content and sensory quality of tobacco leaves increased when the equal proportion of rapeseed cake fertilizer replaced 15%-25% of chemical fertilizer nitrogen, but the yield of tobacco leaves decreased when 35% of chemical fertilizer nitrogen was replaced. The total amount of aroma substances in tobacco leaves was significantly positively correlated with soil organic matter content, the number of bacteria and actinomycetes, and the sensory quality of tobacco leaves. Therefore, the replacement of 15%-25% nitrogen fertilizer with rapeseed cake fertilizer was beneficial to improve soil fertility and tobacco yield and quality in Jiangxi Province.
    Isolation and identification of a strain of Bacillus coagulans and optimizaiton of its sporulation conditions
    WANG Song, WU Cong, CHEN Yan-yan, LI Jia-xin, HAN Yu-tong, XING Li
    2025, 64(12):  78-83.  doi:10.14088/j.cnki.issn0439-8114.2025.12.014
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    A strain of acid-producing Bacillus sp., designated JLNK03, was isolated from the sediment soil from a water pool in this experiment. The strain was identified as Bacillus coagulans through colony and cell morphology observation and 16S rDNA sequencing. To increase the spore production of B. coagulans strain JLNK03,single-factor and orthogonal experiments were conducted to optimize the culture conditions and medium composition for sporulation conditions, and the effects of three sporulation-promoting factors on the sporulation were also investigated. The results showed that the optimal culture conditions for spore production of strain JLNK03 were as follows: culture medium of 12 mL in a 50 mL conical flasks, inocularatio of 3.5% (V/V), initial pH of 6.5, and incubation at 40 ℃ with shaking at 180 r/min for 50 hours. The optimal medium composition consisted of 8 g/L corn starch, 14 g/L soybean powder, 4 g/L magnesium sulfate, and 2 g/L calcium sulfate, supplemented with 2 g/L long-chain inulin as a sporulation-promoting factor. Under this conditons, the average spore count reached (1.13±; 0.02)×; 1010 CFU/mL, which was 3 to 4 orders of magnitude higher than that before optimization. It significantly increased the spore count. This method was relatively simple, and the medium components were cheap and easy to obtain, which could effectively reduce the cost and obtain high-yield spores.
    Chemical and Ecological Responses of Critical Soil Elements under Acidification Stress
    YIN Hong-chang, WANG Ming-rui, CAO Feng, YANG Guo-xiu, YAO Jing-jing
    2025, 64(12):  84-88.  doi:10.14088/j.cnki.issn0439-8114.2025.12.015
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    Soil acidification was posing a significant threat to agricultural production and ecological balance in China. In Hubei Province, due to the influence of topography, climate, and human activities, the soil’s capacity to hold nutrients and convert them into absorbable forms has been weakened, consequently affecting crop yields and quality. To investigate the biochemical impacts of soil acidification on agricultural land and crops, the soil pH across various regions of Hubei Province from 2021 to 2023 was monitored. The results revealed that the southwestern and southern regions experienced the most severe acidification, while the northwestern region exhibited the fastest acidification rate. Two representative areas were selected for systematic analysis of six key indicators: pH, cation exchange capacity, total exchangeable bases, total phosphorus, total aluminum, and available phosphorus. The experiments demonstrated that soil acidification restricted the exchange ability of base cations, leading to soil compaction and impoverishment. Simultaneously, it inhibited the transformation of phosphorus into available forms, resulting in low nutrient use efficiency. If left uncontrolled, this would push the land into a vicious cycle of “acidification-reduced yield-increased fertilizer application-further acidification”.
    Dynamic evaluation of land ecological quality in Suijiang County based on remote sensing ecological index
    PAN Yong-ping, GAO Cheng-jie, CHAO Jiang-qin, LIN Ji-qing
    2025, 64(12):  89-98.  doi:10.14088/j.cnki.issn0439-8114.2025.12.016
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    Based on the remote sensing image data of 2000, 2010, and 2020, the four core indicators of green degree index (NDVI), humidity index (WET), dryness index (NDBSI), and heat index (LST) were extracted through Landsat series satellite images, and the remote sensing ecological index (RSEI) was constructed using the principal component analysis to comprehensively evaluate the land ecological quality of Suijiang County, Yunnan Province, and reveal its spatial and temporal evolution characteristics and driving mechanisms. The results showed that,from 2000 to 2020, the average RSEI of Suijiang County decreased from 0.61 to 0.47, and the ecological quality showed a trend of overall degradation. After 2010, the vegetation restoration effect of Suijiang County was remarkable, and the green degree index continued to increase. The expansion of construction land and the dry and hot climate led to a significant increase in the dryness index and a continuous decline in the humidity index. The ecological degradation of Suijiang County was concentrated in the Jinsha River coastal areas and the areas with the expansion of construction land, while the high-altitude areas with good vegetation protection still maintained high ecological quality.
    Plant Protection
    Isolation and identification of Streptomyces sp. HBERC-20716 and evaluatingits disease prevention and growth-promoting effects on cotton
    LONG Tong, WAN Zhong-yi, WANG Xiao-gang, ZHANG You-chang, AI Lun-qiang, FENG Chang-hui
    2025, 64(12):  99-103.  doi:10.14088/j.cnki.issn0439-8114.2025.12.017
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    A strain of Streptomyces sp. HBERC-20716, which was independently isolated and preserved, was identified, and its efficacy against cotton fusarium wilt as well as its effects on photosynthesis and growth promotion at the cotton seedling stage were evaluated. Through morphological and 16S rRNA gene sequence analyses, the strain was identified as Streptomyces pluricolorescens. Results from indoor pot experiments demonstrated that Streptomyces pluricolorescens HBERC-20716 significantly inhibited the occurrence of cotton fusarium wilt, with a markedly lower seedling blight rate compared to the control. Treatment of cotton seedlings with the fermentation broth of Streptomyces pluricolorescens HBERC-20716 indicated its growth-promoting effect on cotton, as evidenced by plant height, fresh weight, root length, and root weight of the seedlings being significantly greater than those in the control. Measurements of photosynthesis parameters showed that Streptomyces pluricolorescens HBERC-20716 promoted photosynthetic performance, significantly increasing the net photosynthetic rate, transpiration rate, and stomatal conductance, while substantially reducing the intercellular CO2 concentration.
    Construction of a prediction model for Xinyang rice blast based on all subsets regression and BP neural network
    HU Xue-min, ZHU Zhi-gang, JIANG Zhao-qin, JI Xin, CHEN Li-jun, SHI Hong-zhong
    2025, 64(12):  104-109.  doi:10.14088/j.cnki.issn0439-8114.2025.12.018
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    Using meteorological data from the Xinyang City between 2004 and 2021 (excluding 2020), including air temperature, relative humidity, precipitation, and sunshine duration, five key factors influencing rice blast epidemics were identified through correlation analysis. These factors were:The minimum relative humidity in late June, the minimum relative humidity in early May, the minimum temperature in early May, the sunshine duration in mid-June, and the cumulative rainfall in early August. Both all-subset regression and BP neural network algorithms were employed to predict the incidence area of rice blast in the Xinyang City. The results showed that all-subset regression model 1 and model 2 achieved back-testing accuracies of 92.49% and 94.43%, respectively, for the 2004—; 2021 rice blast incidence area, and both yielded a prediction accuracy of 79.68% for the years 2022 and 2023. In comparison, BP neural network models 1 and model 2 achieved back-testing accuracies of 82.72% and 83.55%, respectively, for the 2004—; 2021 period, and prediction accuracies of 98.06% and 95.49% for 2022 and 2023. Based on these results, BP neural network model 1 was identified as the optimal prediction model. Using this model, the predicted incidence area of rice blast in Xinyang City for 2024 was 26 500 hectares(hm2).
    Evaluation of pathogenicity difference of Plasmodiophora brassicae in Yunnan
    ZHANG Shun-rong, LUO Yan-qing, LIU Ya-jun, ZHANG Yun-yun, GAO Jin-xiang, ZHANG Yu-song, YANG Hui, CHENG Xiao-mao, ZU Feng
    2025, 64(12):  110-116.  doi:10.14088/j.cnki.issn0439-8114.2025.12.019
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    Based on an optimized inoculation and identification method for Plasmodiophora brassicae Woronin, five representative strains of the clubroot pathogen from Yunnan Province were selected for indoor inoculation assays to clarify differences in pathogenicity among the strains. Two host materials, Juxin Chinese Cabbage (a highly clubroot-susceptible Chinese cabbage cultivar) and Huayouza 62R (a Brassica napus cultivar highly resistant to physiological race 4 of Plasmodiophora brassicae), were used as inoculation recipients. Pathogenicity differences among the strains were evaluated by analyzing disease incidence and pathogen infection rates. The results showed that infection rates of the different strains on both Juxin Chinese Cabbage and Huayouza 62R differed significantly. Furthermore, the infection rates of all strains increased significantly over the investigation period, but the variation trends in infection rates among strains showed clear differences between the two hosts as the inoculation period extended.
    Horticulture & Local Products
    Effects of comprehensive evaluation of GA3 soaking on seed germination of wild Eurya alata in Danjiangkou
    WANG Xue-ping, YE Fei, WANG Sheng-peng, GAO Shi-wei, ZHANG Bing-hua, ZHENG Peng-cheng
    2025, 64(12):  117-123.  doi:10.14088/j.cnki.issn0439-8114.2025.12.020
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    In order to understand the germination characteristics of Eurya alata Kobuski, the seeds of wild Eurya alata Kobuski in Yanchihe Town, Danjiangkou City were used as experimental materials. The germination rate, germination energy, germination index, germination lag, germination duration, mean germination time, days needed to reach 50% of germination rate(T50), germination uniformity(days from 10% to 90% of germination, T10-90) at different gibberellin(GA3) concentrations (100, 200, 400, 600 mg/L) and soaking times (4, 8, 16, 24 h) were measured. The membership function, principal component analysis, TOPSIS method, combined weighting method based on entropy and coefficient of variation were used to comprehensively evaluate different treatments. The results showed that GA3 could promote the germination of Eurya alata seeds. The concentration of GA3, soaking time and the interaction of different concentrations and times had significant effects on the germination of Eurya alata seeds. Although the evaluation results of different evaluation methods were different, the overall trend was relatively consistent. The treatment of 200 mg/L GA3 with 16 h soaking ranked the highest across different evaluation methods, which was the best treatment combination for Eurya alata seed germination. The germination rate, germination energy and T50 under this treatment achieved the highest values, and the germination index, germination lag and germination uniformity were slightly lower than the highest values. Through comprehensive comparison, the entropy weight-coefficient of variation combined weighting TOPSIS method was identified as the preferred comprehensive evaluation method for the seed germination of Eurya alata. The weight coefficient analysis of the indicators, grey correlation analysis between each index and comprehensive evaluation value both indicated that germination energy, germination index and germination rate had the most obvious influence on the germination of Eurya alata seeds, which were the key indicators for evaluating the effect of the Eurya alata seed germination.
    Preliminary study on the cold resistance physiological mechanisms of five rosa varieties
    CHEN Si-yu, LI Li-mei, JIN Gui-xiang, YU Hai-yuan, DAI Wei, LYU Meng-yan, ZHANG Li-min, CHEN Shi-gang, LI Juan, YU Yang, LIANG Shuang, REN Jun
    2025, 64(12):  124-130.  doi:10.14088/j.cnki.issn0439-8114.2025.12.021
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    Taking five rose varieties including Soeur Emmanuelle, Red Devil, Chocok, Mountain sunrise, and Alpine flame as meterials, during the growing season, the growth vigor of each variety was observed, and changes in physiological and biochemical indicators were measured during the natural cooling process in winter. These indicators included relative conductivity, malondialdehyde (MDA) content, soluble sugar and starch content, as well as the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and phenylalanine ammonia-lyase (PAL). The aim was to clarify the relationship between cold resistance and these physiological and biochemical indicators in different rose varieties. The results showed that only Alpine flame and Mountain sunrise could overwinter normally in Jilin Province, and had a relatively long flowering period. As temperatures decreased, the relative conductivity of all varieties initially increased, then decreased, and then increased again. The MDA content generally showed an upward trend. At -11 ℃, Alpine flame and Mountain sunrise exhibited lower relative electrical conductivity values (68.58% and 70.83%, respectively), indicating that these two varieties could better withstand low temperatures, with less electrolyte leakage and milder cell membrane damage compared to the other three varieties. However, Chocok had an MDA content as high as 88.13 μmol/g at 12 ℃, indicating it could no longer grow normally at this temperature. Proline and soluble sugar content generally increased, while starch content initially rose and then declined. The soluble sugar content of Red Devil and Chocok was consistently significantly lower than that of the other three varieties, indicating weaker resistance to low temperatures. The activities of SOD, POD, CAT, and PAL in all varieties initially increased during the early stages of cooling, demonstrating their role in cold resistance.
    The influence of different fertilization treatments on the anatomical structure of Jue ma
    JIN Xin, LI Tao, LI Jun-qiao, XIE Xin, PENG Mao-zhuo-me
    2025, 64(12):  131-136.  doi:10.14088/j.cnki.issn0439-8114.2025.12.022
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    To investigate the effects of nitrogen, phosphorus, and potassium fertilizers on the anatomical structure of Jue ma(Argentina anserina L.), the study was conducted on the roots, stems, stolons, and leaves of the Qinghai A. anserina No. 3 variety. The results showed that the application of nitrogen fertilizer alone significantly increased the thickness of root phloem, leaf thickness, cortex thickness and vascular bundle area of stolon; while it significantly reduced the thickness of root periderm, xylem thickness, pith area, epidermal thickness and vascular bundle area of the stem. The application of phosphorus fertilizer alone significantly increased the thickness of root periderm, phloem thickness, pith area, vascular cambium thickness, and stem vascular bundle area; while it significantly reduced root xylem thickness, pith thickness, stem epidermal thickness, and stolon epidermal thickness. The application of potassium fertilizer alone significantly increased root xylem thickness, vascular cambium thickness, pith area, leaf thickness, the cortex thickness and vascular bundle area of stolon; while significantly reducing root pith thickness and stem epidermal thickness. Balanced fertilization with nitrogen, phosphorus, and potassium significantly increased root phloem thickness, xylem thickness, pith thickness, pith area, leaf thickness, the epidermal thickness and cortex thickness and vascular bundle area of stolon; while significantly reducing root periderm thickness. Therefore, the application of nitrogen or phosphorus fertilizer alone mainly promoted the development of the vascular cambium and phloem under the expansion of adventitious roots, and potassium fertilizer alone primarily enhanced xylem development, further improving the root’s ability to transport water and nutrients and promoting the growth and development of the A. anserina root system. Balanced fertilization with nitrogen, phosphorus, and potassium could promote the development of roots, stolons, and leaves, thereby improving the quality and stress resistance of A. anserina.;
    Medicinal Plant
    Research progress on the pharmacological effects and mechanisms of cucurbitacin B
    PAN Xiao-fang, GAO Mei-fei, CHEN Rui, HUO Li-ni, ZHU Hai-bin
    2025, 64(12):  137-145.  doi:10.14088/j.cnki.issn0439-8114.2025.12.023
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    The research progress on the biological activities and mechanisms of action of cucurbitacin B (CucB) was reviewed. Studies showed that CucB possessed significant antitumor effects. It was found to regulate multiple signaling pathways, including JAK2/STAT3, PI3K/AKT, MAPK/ERK, and ferroptosis, thereby effectively inhibiting the proliferation, migration, and invasion of various tumor cells (such as those from liver, lung, breast, and gastrointestinal cancers) and inducing cell cycle arrest, apoptosis, and autophagy. In terms of anti-inflammatory effects, CucB could inhibit the activation of the NLRP3 inflammasome and downregulate key pathways like NF-êB, thus alleviating tissue damage in multiple inflammatory models. Furthermore, CucB demonstrated hepatoprotective activity and was shown to ameliorate liver fibrosis through modulating oxidative stress, suppressing inflammatory responses, and regulating axes such as SIRT1/IGFBPrP1/TGF-â; 1. Its potential in alleviating psoriasis was also supported by research, with mechanisms involving the inhibition of pathways such as cGAS-STING and JAK-STAT. Other effects of CucB, including its role in regulating autophagy, suppressing leukemia and osteosarcoma, protecting vascular endothelium, and exhibiting potential neurotoxicity, were also summarized.
    The composition and activity analysis of polysaccharides from Erythropalum scandens Bl. stems and leaves
    WANG Wei-rui, WEI Bing-an, PAN Qiao-dan, HUANG Yuan-he, ZHAO Li-yang, XU Ying-ying
    2025, 64(12):  146-151.  doi:10.14088/j.cnki.issn0439-8114.2025.12.024
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    The monosaccharide composition of polysaccharides from four different parts (old stem, young stem, old leaf, and young leaf) of Erythropalum scandens Bl. was determined using pre-column derivatization high-performance liquid chromatography (HPLC). Their antioxidant properties were comprehensively evaluated by assessing the scavenging capacities against DPPH·, ABTS+·, and superoxide anion radical (O2-·), as well as the ferric ion reducing antioxidant power (FRAP) assay, to screen for highly active polysaccharide components. The results showed that Erythropalum scandens polysaccharides were primarily composed of ten monosaccharides: Namely, mannose, glucosamine, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose. Xylose was not detected in the young stem polysaccharides, but was present in the old stem, old leaf, and young leaf polysaccharides. The total sugar content followed the order of old stem > young stem > young leaf > old leaf. The in vitro antioxidant assays indicated that polysaccharides from all parts exhibited scavenging activity against DPPH·, ABTS+·, and O2-·, as well as ferric ion reducing power. Among them, the old stem polysaccharide demonstrated superior antioxidant activity compared to the other parts.
    Establishment of a rapid propagation system for callus regeneration and seedling formation of Uncaria rhynchophylla
    PAN Li-mei, HUANG Yuan, ZHAN Xin-jie, WAN Ling-yun, WEI Shu-gen, JIANG Ni, XIAO Xiao, LIU Huan-ying, QIAO Le-wen, LI Lin-xuan, ZHANG Zhan-jiang
    2025, 64(12):  152-155.  doi:10.14088/j.cnki.issn0439-8114.2025.12.025
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    Using the tender stem segments of Uncaria rhynchophylla as experimental materials, tissue culture techniques including explant surface sterilization, browning control, callus induction and differentiation, and adventitious root induction were systematically studied. The results showed that the optimal sterilization method for Uncaria rhynchophylla explants was soaking in 75% ethanol for 30 seconds, rinsing with sterile water 3 times, followed by treatment with 0.1% HgCl2 solution for 8 minutes. The addition of 7 mg/L ascorbic acid (VC) effectively reduced explant browning. The optimal medium for callus induction was MS+1.0 mg/L 6-BA+1.5 mg/L 2,4-D; the optimal differentiation medium was MS+0.2 mg/L NAA+2.5 mg/L 6-BA; and the optimal medium for adventitious root induction was 1/2 MS+1.5 mg/L NAA.
    Comparative of agronomic traits, nutritional quality, and antioxidant function of six varieties of Chinese yam
    SUN Rui-min, CHEN Li-na, LA Gui-xiao, LI Xiang-yang, YANG Tie-gang
    2025, 64(12):  156-160.  doi:10.14088/j.cnki.issn0439-8114.2025.12.026
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    Six varieties of Chinese yam (JJH, SY445, SY453, SY449, XBZ, and SY442) were used as materials to systematically compare their field agronomic traits, nutritional quality, and antioxidant functions. The results showed that in terms of agronomic traits, JJH displayed strong growth vigor and the highest yield (60 000.00 kg/hm2); regarding nutritional quality, JJH contained higher levels of crude fiber (1.70%), protein (13.06%), and total sugar (6.44%), whereas SY453 had the highest amylose content (27.86%) and amylose-to-amylopectin ratio (67.54%); in antioxidant function, SY453 displayed the highest hydroxyl radical scavenging rate (96.43%), while JJH showed the lowest (77.59%); SY449 exhibited the strongest performance in both ferric ion reducing power and ABTS radical scavenging rate (95.44%), followed by SY453, the remaining four varieties demonstrated relatively low antioxidant capacity without significant differences. In summary, SY449 showed superior comprehensive performance in antioxidant function, whereas JJH, with high yield and crisp texture, was more suitable for fresh consumption or stir-frying; SY449, with medium yield and soft texture, was more suitable for steaming, boiling, or as a raw material for functional foods.
    Effects of citrus varieties and processing methods on the yield and quality of tangerine peel
    LI Nian-zu, WANG Tao, LI Rong-fang, YAO Yu-ling, LI Yun-fei, YANG Ying-chun, WANG Feng
    2025, 64(12):  161-165.  doi:10.14088/j.cnki.issn0439-8114.2025.12.027
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    To compare the effects of different citrus(Citrus reticulata Blanco) varieties and processing methods on the yield and quality of tangerine peel, the processing technology of tangerine peel was aimed to be optimized. Eight citrus varieties planted in Hubei Province were used as test objects. Two peel pretreatment methods(scalding and non-scalding) and four drying conditions(45, 55, 65 ℃, and room temperature shade-drying) were adopted for processing. The indicators including peel extraction rate, drying matter rate, and hesperidin content were measured. The results showed that in terms of yield factors, the variety had an extremely significant effect on the peel extraction rate of tangerine peel, while pretreatment had no significant effect; both pretreatment and variety had an extremely significant effect on the dry matter rate of tangerine peel; the interaction between variety and pretreatment had an extremely significant effect on peel extraction rate but no significant effect on dry matter ratio. Regarding hesperidin content, under the same pretreatment conditions, drying temperature had no significant effect on the hesperidin content of the same variety; scalding pretreatment significantly reduced the hesperidin content of Dapu, Rinan No.1, and Shijihong; different pretreatments and drying temperatures had no significant effect on the hesperidin content of Dafen No.4, Guoqing No.1, Xingjin, and Guijing. Multifactor analysis of variance showed that both variety and pretreatment had an extremely significant effect on hesperidin content, with variety having the greatest effect, while the main effect of drying temperature was not significant; the interaction between variety and pretreatment had an extremely significant effect on hesperidin content, and the three-way interaction among variety, pretreatment, and drying temperature was not significant.
    Animal Husbandry & Veterinary Medicine
    Isolation, identification and drug sensitivity test of Streptococcus suis strain 9 in Hubei Province
    GUO Rui, MA Rui, YUAN Fang-yan, LIU Wei, YANG Ke-li, LIU Ze-wen, GAO Ting, LI Chang, WU Qiong, ZHU Jia-jia, WANG Chong, TIAN Yong-xiang, ZHOU Dan-na
    2025, 64(12):  166-169.  doi:10.14088/j.cnki.issn0439-8114.2025.12.028
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    The pathogenic bacteria was isolated from nursery piglets exhibiting acute fatal symptoms of streptococcosis at a pig farm in Hubei Province. The pathogen was identified through bacterial culture, morphological observation, biochemical tests, and molecular analysis. After inoculating clinical samples onto TSA medium supplemented with 5% sheep blood, Gram-positive cocci arranged in chains were isolated. PCR amplification using serotype 9-specific primers and subsequent sequencing confirmed the isolate as Streptococcus suis serotype 9, designated strain XF-1. Virulence gene screening revealed that XF-1 carried fbps, orf, sly, and gapdh genes, while epf and mrp genes were negative. Antimicrobial susceptibility testing indicated intermediate sensitivity to cefuroxime, ceftiofur, and ampicillin, but resistance to 12 antibiotics including cephalothin and gentamicin. Mouse pathogenicity trials demonstrated high virulence, all mice challenged with ≥; 5×; 108 CFU died within 7 days (groups 20×; 108, 10×; 108, and 5×; 108 CFU), while the 1×; 108 CFU group showed 40% mortality. Bacteria re-isolated from deceased mouse organs were PCR-confirmed as identical to XF-1.
    Effects of aluminum stress on seed germination and seedling growth of four Medicago species
    XIONG Jun-bo, ZHANG He-shan, TIAN Hong, LU Jiao-yun, WU Xin-jiang, LIU Yang
    2025, 64(12):  170-174.  doi:10.14088/j.cnki.issn0439-8114.2025.12.029
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    To compare the physiological responses of different Medicago species to aluminum (Al) stress and identify germplasm with strong Al tolerance, four species M. polymorpha, M. lupulina, M. minima, and M. sativa were subjected to Al3+ concentrations of 0, 50, 100, and 150 μmol/L. Germination rate, root and shoot dry weights, Al content, and antioxidant enzyme activities were measured. Al stress significantly inhibited seed germination and seedling growth in all species (P<; 0.05), with root biomass showing the largest reduction, indicating that roots were the primary target of Al toxicity. M. polymorpha exhibited the highest Al tolerance, maintaining higher germination rate, biomass, and antioxidant enzyme activities and lower Al accumulation and malondialdehyde (MDA) content. Correlation analysis revealed significant negative relationships between Al content and growth parameters, suggesting that reduced Al accumulation and enhanced antioxidant defense contribute to Al tolerance. These findings highlighted M. polymorpha as a promising species for improving Al tolerance in Medicago breeding and for cultivation in acidic soils in southern China.
    Storage & Processing
    Research on optimization of ultrasonic-assisted extraction process of flavonoids from Stephania kwangsiensis H. S. Lo and its antioxidant activity
    HUANG Shao-jun, HUANG Shi-ya, LI Tai-qiu, HUANG Hao, ZHOU Lan, LI Wan
    2025, 64(12):  175-180.  doi:10.14088/j.cnki.issn0439-8114.2025.12.030
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    To optimize the extraction process of total flavonoids from the tubers of Stephania kwangsiensis H. S. Lo and explore its in vitro antioxidant activity, based on single-factor experiments, an orthogonal test method was used to investigate the effects of ethanol concentration, solid-liquid ratio, extraction temperature, and ultrasonic time on the extraction rate of total flavonoids. The optimal extraction process was determined, and the in vitro antioxidant activity of total flavonoids was further studied. The results showed that the optimal extraction condition for total flavonoids from the tubers of Stephania kwangsiensis H. S. Lo was ethanol concentration of 70%, solid-liquid ratio of 1∶; 35 (g/mL), extraction temperature of 50 ℃, and ultrasonic time of 50 min. Under this condition, the yield of total flavonoids content could reach up to 5.45 mg/g. The total flavonoids from Stephania Kwangsiensis H. S. Lo exhibited strong antioxidant activity, with the scavenging rate against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals reaching up to (84.39±; 0.61)%. In this study, the ultrasound-assisted extraction process of total flavonoids coupled with ultraviolet spectrophotometry was simple and yielded reliable results. Given that the total flavonoids possessed good antioxidant properties, this method could provide a sound theoretical reference for the extraction, development, and application of total flavonoids from Stephania kwangsiensis H. S. Lo.
    Study on super high pressure assisted deep eutectic solvent extraction of total flavonoids from Rabdosia rubescens (Hemsl.) Hara and its antioxidant activity
    WANG Le, ZHANG Pei-qi, ZONG Wei
    2025, 64(12):  181-186.  doi:10.14088/j.cnki.issn0439-8114.2025.12.031
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    To optimize the extraction process of total flavonoids from Rabdosia rubescens (Hemsl.) Hara in super high pressure assisted deep eutectic solvent (DES) and analyze its antioxidant activity, the extraction condition was optimized by single factor experiments and response surface experiments. The scavenging activity of the extracted flavonoids against 1,1-dipyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-ulfonic acid) diammonium salt (ABTS) free radicals was analyzed. The results showed that the optimal condition for the extraction of total flavonoids in super high pressure assisted deep eutectic solvent was material to liquid ratio of 1∶; 40 (g/mL), water content in DES of 30% ,extraction pressure of 402 MPa, and extraction time of 5 min, under which the yield of total flavonoids from the Rabdosia rubescens (Hemsl.) Hara was 9.21%. The half-inhibitory concentrations of the prepared total flavonoids of Rabdosia rubescens (Hemsl.) Hara for the scavenging effects of DPPH· and ABTS·were 0.136 mg/mL and 0.123 mg/mL, respectively. The research could provide theoretical support for the development of total flavonoids of Rabdosia rubescens (Hemsl.) Hara and the study of antioxidant activity.;
    Effects of 60Co-γ ray irradiation on color and antioxidant activity of purple cabbage
    QIU Ya-lu, WANG Yan, GAO Peng, XU Pan, HE Jiang
    2025, 64(12):  187-193.  doi:10.14088/j.cnki.issn0439-8114.2025.12.032
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    Purple cabbage was used as the research object to investigate the change of color and epidermal morphology structure, anthocyanin composition and antioxidant activity under; 60Co-γ irradiation, so as to evaluate the effects of different irradiation doses on the physiological and biochemical activity of anthocyanin in purple cabbage. The results showed that purple cabbage anthocyanins were sensitive to radiation, and a dose of 1 kGy could change the various components of anthocyanins, but there was no significant difference in the total content of anthocyanins. Irradiation did affect the antioxidant capacity of purple cabbage, and the impact of 1 kGy irradiation was more pronounced than that of 2 kGy irradiation. After irradiation, the activity of the key enzyme POD in the anthocyanin metabolism pathway significantly increased. There was no significant difference in the DPPH radical scavenging ability and PAL activity of purple cabbage irradiated with a dose of 2 kGy compared to non irradiated samples. The irradiation of 2 kGy increased the brightness axis parameter value of the leaves and made the leaf color brighter. In summary, 2 kGy of; 60Co-γ irradiation was the optimal dose for preserving purple cabbage and could delay the fading of fresh cut vegetable and fruit products to a certain extent.
    Detection Analysis
    Analysis of rare earth elements in soil and different growth years of earthworms karst areas
    LYU Chu-han, SU Hua-sheng, ZAN Huan-nan, LI Ting
    2025, 64(12):  194-198.  doi:10.14088/j.cnki.issn0439-8114.2025.12.033
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    To analyze the distribution patterns, enrichment characteristics, temporal dynamic content patterns, and correlations between 14 rare earth elements in soil systems of karst areas and different growth years of earthworms and to provide scientific basis for the health risk assessment and enrichment characteristics of rare earth elements in Dilong. Samples of different ages of earthworms and soil were collecting, and using inductively coupled plasma mass spectrometry (ICP-MS) to determine the content of rare earth elements in earthworms and soil of different ages. Results showed that the distribution patterns of soil rare earth elements and 14 rare earth elements in earthworms showed significant inheritance. They did not undergo significant fractionation during transport within the earthworms, and the biological enrichment coefficient was only 0.01-0.02. There was no significant correlation between rare earth element content and growth age, but there was a significant positive correlation between elements. The rare earth element content in earthworms was in the order of Ce>La>Nd>Pr>Gd>Sm>Dy>Eu>Er>Yb>Tb>Ho>Tm>Lu. The distribution characteristics of rare earth elements in earthworms were clearly indirectly regulated by soil for bioaccumulation, and their enrichment ability was weak. The acceptable daily intake (ADI) of rare earth elements in earthworms was far below the safety limit of 0.07 mg/(kg·d), and did not pose a health risk. This provided a reference for soil aquaculture of earthworms in karst areas.
    Residual behavior and dietary risk assessment of pyraclostrobin seed treatment in peanuts
    CHEN Xin, LYU Ang, ZHU Kun-miao, LIU Li, XIA Hong, LIU Jun
    2025, 64(12):  199-203.  doi:10.14088/j.cnki.issn0439-8114.2025.12.034
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    An analytical method for determining pyraclostrobin residues in peanut kernels and peanut straw was established, and the dietary safety of pyraclostrobin seed treatment in peanut production was evaluated. The samples were homogenized and extracted with acetonitrile, followed by phase separation with magnesium sulfate and sodium acetate, and cleaned up using C18 and primary secondary amine (PSA). The residue levels of pyraclostrobin in peanut kernels and peanut straw were determined by UPLC-MS/MS. The long-term dietary intake risk was assessed by combining the residue data with the dietary structure of the Chinese general population. The results indicated that pyraclostrobin showed a good linear relationship in the concentration range of 0.005-0.200 mg/L (correlation coefficient ≥; 0.999 9). The average fortified recoveries of pyraclostrobin in peanut kernels and peanut straw were 73%-83% and 66%-84%, with relative standard deviations of 1%-3% and 3%-5%, respectively. The limit of detection and the limit of quantification were 1.4×; 10-5 ng/μL to 2.1×; 10-5 ng/μL and 0.01 mg/kg, respectively, which met the technical requirements for pesticide residue analysis in crops. The field residue trial results showed that the supervised trial median residue (STMR) and the highest residue (HR) of pyraclostrobin in peanut kernels were both 0.010 mg/kg at two application rates. The STMR in peanut straw was 0.010 mg/kg, while the HR increased from 0.053 mg/kg to 0.073 mg/kg with the increasing application rate. The dietary risk assessment revealed that the long-term dietary intake risk quotient (RQ) was 38.7% for the current dietary pattern of Chinese residents. The method was convenient, sensitive, and accurate, making it suitable for monitoring pyraclostrobin residues in peanuts. Under the recommended dosage, the residues of pyraclostrobin in peanuts posed an acceptable long-term dietary intake risk to the general population.;
    Analysis of volatile components in leaves and twigs of Machilus thunbergii and Machilus yunnanensis
    CHEN Bo-meng, LI Ting, LIU Li, JIANG Xin-yi, LIU Duo-min, ZHANG Cai-ze, ZHANG Jian-ping, CHEN Yu-ting, CHENG Fei, MA Wen-ping
    2025, 64(12):  204-211.  doi:10.14088/j.cnki.issn0439-8114.2025.12.035
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    To investigate the volatile components in the leaves and twigs of Machilus thunbergii and Machilus yunnanensis, seedlings from the nursery of Nanning Liangfengjiang National Forest Park in Nanning City, Guangxi Zhuang Autonomous Region, were used as materials. The leaves and one-year-old twigs were collected, and the volatile components were extracted using the n-hexane immersion method, followed by analysis via gas chromatography-mass spectrometry (GC-MS). The results showed that the components with the highest relative content in Machilus thunbergii leaves, Machilus thunbergii twigs, Machilus yunnanensis leaves, and Machilus yunnanensis twigs were β-elemene (10.49%), β-eudesmol (16.60%), tetracosane (11.22%), and δ-cadinene (9.88%), respectively. The relative content of terpenoids was dominant in both the leaves and twigs of Machilus thunbergii, accounting for 74.82% and 77.29%, respectively. The leaves had a higher relative content of aromatic hydrocarbons (11.05%), while the twigs had a higher relative content of alcohols (18.94%). The relative content of terpenoids in Machilus yunnanensis leaves was 38.78%, which was significantly lower than that in Machilus thunbergii leaves, Machilus thunbergii twigs, and Machilus yunnanensis twigs. However, the relative content of alkanes was higher (27.51%), and it also contained a considerable amount of alcohols, ketones, and esters. The relative contents of terpenoids, alkanes, and organic acids in Machilus yunnanensis twigs were relatively high, at 57.50%, 20.52%, and 15.88%, respectively. The leaves and twigs of both Machilus thunbergii and Machilus yunnanensis contained abundant volatile components, and the relative contents of these components varied among different tree species and different plant parts.
    Information Engineering
    A detection method for potato shape of commercial potatoes based on quantum-behaved particle swarm optimization and support vector machine
    WAN Peng, XIONG Cheng-xin, CAI Jie, LIU Yan-fang, WU Xiao-long
    2025, 64(12):  212-217.  doi:10.14088/j.cnki.issn0439-8114.2025.12.036
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    Aiming at the problems of low accuracy and poor efficiency of existing potato shape detection methods for commercial potatoes, which made it difficult to meet the requirements of mechanized and automated sorting, a method for potato shape detection was investigated based on machine vision technology. Three potato shapes (spherical or subspherical, ellipsoidal, and irregular) were taken as research objects. First, a potato image acquisition system was built to collect images of the three shapes. Then, the images were preprocessed to obtain potato contour images. Subsequently, ten invariant moments of the potato images were extracted as feature parameters, and thesupport vector machine classifier was optimized using the quantum-behaved particle swarm optimization algorithm, ultimately achieving automatic detection of potato shape. The results showed that the detection accuracies for spherical or subspherical, ellipsoidal, and irregular potato shapes in the QPSO-SVM model were 97.0%, 94.0%, and 91.0%, respectively, with an average detection accuracy of 95.2%. This model could be used for the rapid detection of potato shape.
    Evolution, application efficiency, and future prospects of YOLO series models in wheat ear detection
    WANG Qing, WANG Zhi-qiang, LIU Hong, LIANG Min, ZHANG Yu-chen, LIN Yu
    2025, 64(12):  218-227.  doi:10.14088/j.cnki.issn0439-8114.2025.12.037
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    By reviewing YOLO series models and their application research in wheat ear monitoring at home and abroad, it was found that these models, with their excellent real-time performance and high precision advantages, had become a research hotspot in the field of agricultural intelligent perception.Through continuous model optimization and improvement, the accuracy and efficiency of wheat ear detection were continuously improved, providing strong support for the intelligent prediction of wheat yield and the development of agricultural modernization. Although YOLO series models had made significant progress in wheat ear monitoring, there was still room for improvement in lightweight deployment, multi-modal data fusion, and cross-scene generalization ability. Future research should focus on the above directions to further enhance the practicality and robustness of the models, providing more efficient and reliable technical support for smart agriculture.
    Biological Engineering
    Callus induction and plant regeneration from different explants of Aquilaria sinensis
    YUAN Chang-ge, ZHOU Ya-qin, QIN Shou-jie, ZHANG Miao, FU Peng, TAN Xiao-ming
    2025, 64(12):  228-234.  doi:10.14088/j.cnki.issn0439-8114.2025.12.038
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    Using the leaf,stem,bud, and root of Aquilaria sinensis(Lour.) Gilg. as experimental materials, an orthogonal experimental design was employed via DPS software. By applying different types and concentrations of plant growth regulators (6-BA, 2,4-D, TDZ, NAA, KT), the effects of growth regulators combinations and their concentrations on callus induction, differentiation, and rooting of Aquilaria sinensis were investigated. The results showed that the optimal explant for inducing Aquilaria sinensiscalluswas theleaf, with the highest induction rate of 100%. The optimal medium for leafcallus induction was WPM + 6-BA 1.5 mg/L + 2,4-D 0.5 mg/L + NAA 0.8 mg/L + KT 0.2 mg/L + TDZ 1.0 mg/L. The optimal medium for adventitious bud induction ofAquilaria sinensiswas WPM + 6-BA 0.5 mg/L + NAA 0.2 mg/L + sucrose 20 g/L + agar 6 g/L. The callus from buds and stems could differentiate into adventitious buds. The optimal medium for adventitious bud induction was WPM + 6-BA 0.5 mg/L + NAA 0.2 mg/L + sucrose 20 g/L + agar 6 g/L, resulting in a bud induction rate of 72.22% and an average of 5.68 adventitious buds per explant. The optimal medium for root induction was 1/2 MS + NAA 1.0 mg/L + sucrose 20 g/L + agar 6 g/L, achieving a rooting rate of 46.67%.
    Identification and expression analysis of the alternative oxidase family gene in Amorphophallus konjac
    GUAN Wan-ting, HE Fei, LUO Chang-xin, YI Qian, ZHAO Xiao-jie, PU Xing-yan, SUN Ke-xin, ZHU Feng-yan, CHU Hong-long
    2025, 64(12):  235-243.  doi:10.14088/j.cnki.issn0439-8114.2025.12.039
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    The alternative oxidase(AOX) family gene in Amorphophallus konjac K.Koch was analyzed at the whole-genome level. The results showed that there were four alternative oxidase family gene members in Amorphophallus konjac, named AkAOX1 to AkAOX4. Their protein length ranged from 330 to 361 aa, molecular weights from 37 087.52 to 41 243.83 u, and isoelectric points from 5.97 to 7.27. They were primarily located in the cytoplasm and chloroplast. The Amorphophallus konjac alternative oxidase family gene members were divided into two subfamilies, namely the AOX1 subfamily (AkAOX1, AkAOX2, AkAOX3) and the AOX2 subfamily (AkAOX4). Gene collinearity analysis indicated no potential duplicated gene pairs or tandem duplication events in Amorphophallus konjac. Ka/Ks analysis further showed that these homologous gene pairs were primarily under purifying selection during evolution. The promoter regions of the Amorphophallus konjac alternative oxidase family gene contained three types of cis-acting elements, associated with plant hormone response, growth and development response, and environmental (stress) response. The qRT-PCR results showed that the expression level of the AkAOX1 gene was significantly up-regulated under soft rot pathogen infection, drought stress, and salt stress, while the expression level of the AkAOX4 gene was significantly down-regulated under soft rot pathogen infection. It was speculated that the Amorphophallus konjac alternative oxidase family gene played important roles in its growth and development, hormone signal transduction, and responses to biotic and abiotic stresses.
    Economy & Management
    Decision analysis of cooperative innovation of new agricultural products based on government regulation
    CHENG Cheng
    2025, 64(12):  244-250.  doi:10.14088/j.cnki.issn0439-8114.2025.12.040
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    Considering that in the process of processing and production of traditional agricultural products, there would be surplus of agricultural products among enterprises due to the aging of traditional agricultural products, which would affect the freshness. Based on the game theory, the optimal decision of agricultural product supply chain composed of new agricultural product manufacturers and suppliers on agricultural product freshness technological innovation was studied, and under the government regulation, the overall profit, output and influence of agricultural product supply chain in non-cooperative innovation and cooperative innovation were analyzed. Through the analysis, it was concluded that the profit, output and freshness effect of cooperative innovation were the best, and the government regulated the enterprise behavior through encouragement and punishment, which could make enterprises actively invest in technological innovation, and some corresponding countermeasures and suggestions were put forward.
    The impact mechanism of agricultural mechanization on rural household income gap and policy implications
    FU Zhi-yuan, QIAN Wei
    2025, 64(12):  251-256.  doi:10.14088/j.cnki.issn0439-8114.2025.12.041
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    Based on the data from the China Labor-force Dynamic Survey (CLDS) in 2014 and 2016, this study first employed the stochastic frontier analysis (SFA) model to measure the agricultural total factor productivity of rural households, and then systematically examined the impact of agricultural mechanization on the income gap of rural households in China and its underlying mechanism by integrating recentered influence function (RIF) regressions with a mediation effect model. The results indicated that agricultural mechanization significantly narrowed the rural household income gap, and the effect of full mechanization was stronger than that of semi-mechanization. Agricultural mechanization not only had a direct effect but also generated a significant indirect effect by enhancing agricultural total factor productivity, demonstrating a partial mediation effect. This effect exhibited significant heterogeneity; it was more pronounced among households receiving agricultural subsidies but weaker among those without subsidies, and it was prominent in plain areas but weaker in hilly or mountainous areas.Based on the findings, this study recommended improving the socialized service system for agricultural machinery, enhancing the targeting precision of subsidy policies, and promoting the coordinated development of “adapting machinery to local conditions and modifying land for machinery use” to elevate the level of mechanization in mountainous areas, narrow the rural household income gap, and contribute to the achievement of common prosperity.;