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Table of Content

    04 March 2026, Volume 65 Issue 2
    Breeding & Cultivation
    Effects of melatonin priming on drought resistance of dryland wheat at seed germination stage
    WU Xiu-ning, TANG Yun-xia, DUAN Xiao-ping, GAO Jing-xuan, GAO Bao-yun, ZHANG Jun, WANG Xin-jun
    2026, 65(2):  1-4.  doi:10.14088/j.cnki.issn0439-8114.2026.02.001
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    In order to clarify the regulatory effect of melatonin on the drought resistance of dryland wheat (Triticum aestivum L.) during seed germination stage, the dryland wheat variety Shangmai 5226 was used as the experimental material to determine the effects of different concentrations (0, 75, 150 and 300 μmol/L) of melatonin priming on seed germination and seedling physiological indexes under different drought intensities [0, 10%, 15%, 20% and 25% of polyethylene glycol (PEG-6000)]. The results indicated that with the intensification of drought stress, germination potential, germination rate, seedling water content, coleoptile length, radicle length, radicle number, and chlorophyll content showed a decreasing trend, while soluble sugar and proline contents increased. Compared with the drought-stressed treatments that did not receive priming, melatonin priming at 75 and 150 μmol/L increased germination potential and germination rate to varying degrees under 10%, 15%, and 20% PEG-6000 treatments (except for germination rate under 20% PEG-6000), and melatonin priming at 75, 150, and 300 μmol/L significantly enhanced soluble sugar content under 10% and 15% PEG-6000 treatments. The comprehensive evaluation using the membership function method showed that under melatonin priming at 75 and 150 μmol/L, the comprehensive evaluation values for the 10% and 15% PEG-6000 treatments were higher than those of the corresponding drought-stressed treatments without priming; similarly, under 300 μmol/L melatonin priming, the values for the 15% and 25% PEG-6000 treatments were higher than those of their respective controls without priming. In conclusion, 75 and 150 μmol/L melatonin priming could effectively alleviate the drought stress of 10% and 15% PEG-6000 intensities.
    Photosynthesis and yield performance of potato under different cultivation modes in alkaline saline-alkali soil of Dongying City
    CHEN Guang-xia, ZHAO Peng, ZHANG Zhi-kai, YANG Xiao-hui, YANG Yu, WANG Yong-qiang, DONG Dao-feng, LIU-fang
    2026, 65(2):  5-8.  doi:10.14088/j.cnki.issn0439-8114.2026.02.002
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    In order to improve the utilization rate of saline-alkali land and effectively increase the yield of potato in alkaline saline-alkali land, the experiment was carried out in alkaline saline-alkali land of Dongying City, Shandong Province, and six different cultivation modes were adopted to investigate their effects on botanical traits, photosynthesis and yield of potato. The results showed that the treatment of sowing with a furrow opening of 10 cm + drip irrigation + film mulching + whole tuber planting had the best comprehensive performance, exhibiting the earliest emergence time, the highest emergence rate and yield, and the strongest photosynthetic capacity, with a converted yield of 34 660 kg/hm2. The second-best performance was observed in the treatment of flat sowing + 40 cm-high ridge + drip irrigation + plastic film mulching + whole tuber planting treatment,with a converted yield of 34 589 kg/hm2. This showed that in the saline-alkali soil environment, the whole potato sowing, drip irrigation, and film mulching had a prominent role in promoting potato growth.
    Key points and benefit analysis of planting and breeding of the“crayfish + rice + Macrobrachium rosenbergii” new cultivation model in Hubei Province
    WANG Ben-fu, WANG Ju-guang, TIAN Mao-tao, XU Wei-jie, ZHANG Zuo-lin, LI Yang, ZHANG Zhi-sheng, ZHANG jun, CHENG Jian-ping
    2026, 65(2):  9-15.  doi:10.14088/j.cnki.issn0439-8114.2026.02.003
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    In response to issues in conventional rice-crayfish co-culture systems, such as the susceptibility of the second batch of crayfish to high temperatures and diseases, as well as low aquaculture yields, a new comprehensive rice-crayfish co-culture model “crayfish (Procambarus clarkii)+ rice (Oryza sativa L.)+ Macrobrachium rosenbergii” (one rice crop and two shrimp species) was introduced. Compared with the conventional rice-crayfish co-culture model, this model replaced crayfish with Macrobrachium rosenbergii in the perimeter trenches during the high-temperature period from June to October. Without affecting rice yield, the utilization rate of the ring ditch in the paddy field increased by 21.1%, and the utilization rate of the accumulated temperature in the system increased by 53.7%. The yield of this model was 2 381.2 kg/hm2 for crayfish, 877.5 kg/hm2 for Macrobrachium rosenbergii, and 8 250.2 kg/hm2 for high-quality rice. The total output value was 113 550 yuan/hm2, and the income was 55 650 yuan/hm2, which was 15 780 yuan/hm2 higher than that of conventional rice-crayfish co-cropping. It had great market potential and development prospects.
    Resource & Environment
    Multi-scenario simulation of land use change and its impact on ecosystem service values in the Ili River Valley
    MA Ao, LIU Zhi-you, SUN Yi, QIN Yu, LI Jian-qiang, HE Hua, YANG Hong-bin
    2026, 65(2):  16-26.  doi:10.14088/j.cnki.issn0439-8114.2026.02.004
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    Based on the land use data of the Ili River Valley from 2000 to 2020, the PLUS model was used to simulate the land use evolution patterns under four scenarios of natural development, economic development, ecological protection, and planning intervention in 2030, and the temporal and spatial dynamic changes and spatial agglomeration characteristics of ecosystem service value (ESV) under each scenario were calculated and quantitatively analyzed. The results showed that under the four development scenarios, the ESV in 2030 in the study area was shown as ecological protection scenario > planning intervention scenario > natural development scenario > economic development scenario. Under the natural development scenario, the area of forest land and grassland decreased significantly;under the economic development scenario, construction land expanded rapidly; under the ecological protection scenario, the rate of forest land reduction slowed down, and under the planning intervention scenario, the area of cultivated land increased substantially. The spatial distribution pattern of ESV in Yili River Valley under multi-scenario simulation in 2020 and 2030 was relatively stable. The high-value area increased under the planning intervention scenario, and the high-value area in the north and south-central region changed to the low-value area under the economic development scenario. The extremely significant hot spots of ESV were mainly distributed in the Ili River Basin and the southern Tianshan Mountains, and the extremely significant cold spots were mostly distributed in the central and northern parts of the valley, corresponding to the spatial pattern of land use. Scientific planning and layout of future land use was the key path to enhance regional ESV and maintain a good ecological environment.
    Effects of potassium-reduced application of biochar-based organic-inorganic compound fertilizer on dissolved organic matter and potassium availability in tobacco-planting soil
    DIAO Han-qian, TIAN Wei-qiang, ZHANG Xiao-tao, LU Hui-jun, ZHANG Min, QI Yong-bo
    2026, 65(2):  27-35.  doi:10.14088/j.cnki.issn0439-8114.2026.02.005
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    In order to explore the effects of potassium-reduced application of biochar-based organic-inorganic compound fertilizer (BOCF) on dissolved organic matter (DOM) characteristics and potassium availability in tobacco-planting soils, pot experiments were conducted using two tobacco-planting soil types (paddy soil and upland yellow soil). Four treatments were set up, including conventional organic-inorganic compound fertilizer (CK), BOCF with equivalent nutrients (T1), BOCF with a 10% K2O reduction (T2), and BOCF with a 15% K2O reduction (T3). At the maturity stage of flue-cured tobacco, the physicochemical properties, the DOM spectroscopic characteristics, and the potassium fractionation of the soil were analyzed. The results showed that the response of soil potassium availability to potassium reduction varied with soil type. In paddy soil, the water-soluble potassium (WSK) content of T1, T2 and T3 was significantly reduced by 24.7%, 34.5% and 40.3% respectively, compared with CK. The non-exchangeable potassium (NEK) content of T1,T2 and T3 was lower than that of CK, with the T3 showing a significant decrease of 40.4%. The content of various forms of potassium in yellow soil was relatively stable, and there was no significant difference. Each BOCF treatment enhanced the accumulation of humic-like substances and the humification degree in DOM of both paddy and yellow soils. Correlation and redundancy analyses indicated that changes in the chemical properties of DOM in both soils partially regulated soil potassium supply. Therefore, the utilization of potassium-reduction techniques with biochar-based organic-inorganic compound fertilizer proved to be more viable in yellow soil for maintaining potassium stability, but led to the consumption of the non-exchangeable potassium pool in paddy soil.
    Effects of biochar and polyacrylamide addition on the water retention characteristics of red soil and the drought resistance characteristics of Paspalum wettsteinii
    YAO Wei-fang, DAI Hao-sheng, HUA Xue-ying, XU Peng, YU Jing
    2026, 65(2):  36-41.  doi:10.14088/j.cnki.issn0439-8114.2026.02.006
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    In order to construct a set of technical system that could effectively improve the structure of red soil and improve the efficiency of vegetation restoration, the effects of biochar (2.0%) and polyacrylamide (PAM, 0.5%) addition on soil structure, physiology and growth of Paspalum wettsteinii were studied in red soil of southern China. The results showed that, compared with the control without soil conditioner(CK), the saturated hydraulic conductivity of soil treated with PAM increased by 18.4%, while that of the mixed treatment of PAM and biochar decreased by 42.4%. Single application of PAM or biochar could not increase the capillary water retention capacity and field water retention capacity of red soil. The mixed application of PAM and biochar significantly increased the capillary water retention capacity, field water retention capacity and capillary porosity of red soil. Under drought conditions, compared with CK, the mixed application of PAM and biochar significantly reduced the malondialdehyde content in the leaves of Paspalum wettsteinii, and significantly increased the water content in the leaves and the plant height of Paspalum wettsteinii. In summary, the addition of 2.0% biochar and 0.5% PAM showed the best effect in improving the structure of red soil and promoting the growth of Paspalum wettsteinii.
    Effects of different irrigation amounts on the growth and yield of winter potato under integrated water and fertilizer management
    LI Si-yu, FU Yan-yan, WEI Wei, WU Xu, LI Xiao-yuan, LI Xu, LU Yao
    2026, 65(2):  42-47.  doi:10.14088/j.cnki.issn0439-8114.2026.02.007
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    In response to the need for synergistic optimization of water and fertilizer in the water-deficient areas during winter of the Yunnan Plateau, the effects of different irrigation amounts on the growth traits, marketable tuber rate and yield of winter potato (Solanum tuberosum L.) were systematically investigated based on the integrated water and fertilizer management under mulch drip irrigation. Using Zhaoshu No. 12 as the test material, treatments included a traditional management model (control, 100% irrigation amount) and five irrigation gradients (T5 to T1, 60% to 100% irrigation amount) under the integrated water and fertilizer management. The results showed that the irrigation amount had no significant effect on the emergence rate and marketable tuber rate of winter potato, but both showed a downward trend as the irrigation amount decreased. Plant height decreased with the reduction of irrigation amount, and the T4 (70% irrigation amount) and T5 (60% irrigation amount) treatments were significantly lower than the other treatments. The tuber number per plant was the highest in the T3 (80% irrigation amount) treatment and was significantly higher than that in the control treatment. The single tuber weight, yield, and aboveground biomass were the highest in the T1 (100% irrigation amount) treatment and showed a decreasing trend as the irrigation amount decreased. Correlation analysis indicated that yield was significantly positively correlated with aboveground biomass, single tuber weight, emergence rate, and irrigation amount. Under the integrated water and fertilizer management with mulch drip irrigation, maintaining a 100% irrigation amount could maximize the growth and yield potential of winter potato, representing an effective strategy for achieving high yield and quality production in the region.
    Effects of modified fruit-wood biochar on phosphorus availability in calcareous soil and wheat growth
    FAN Yi-xuan, JIANG Wen-qian, LI Ze-yu, WANG Xu-dong, ZHANG Yu-lin
    2026, 65(2):  48-56.  doi:10.14088/j.cnki.issn0439-8114.2026.02.008
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    To investigate the regulatory effects of fruit-wood biochar modified by different chemical methods on phosphorus availability in calcareous soil and wheat growth, and to optimize the application strategy of biochar in phosphorus management, the effects of fruit-wood biochar modified by KH2PO4, citric acid, and HCl on the available phosphorus content in calcareous soil, the dry weight of wheat, and the phosphorus uptake in wheat shoots were systematically evaluated through an incubation experiment and a pot experiment. The results showed that among the three modified treatments, there was no significant difference in the available phosphorus content between the rinsed and unrinsed biochar, so the unrinsed biochar was uniformly used in the subsequent experiments. The modification with KH2PO4 significantly increased the available phosphorus content of the biochar. After application to the soil, it could release phosphorus, causing the soil available phosphorus content to reach a peak at 14 days and maintain a relatively high level for a long period. At an application rate of 2.0%, a second release peak appeared at 40 days, demonstrating a good slow-release property. The biochar modified by KH2PO4 showed the best effect at an application rate of 1.0%, which not only significantly increased the dry weight of wheat but also significantly enhanced the phosphorus uptake in wheat shoots. After the application of biochar modified by citric acid or HCl to the soil, there was no obvious change in the soil available phosphorus content. The biochar modified by KH2PO4 was more suitable as a slow-release phosphorus carrier for the amelioration of calcareous soil.
    Effects of lime application on selenium speciation in selenium-rich soil and selenium accumulation in sorghum in the Southwest karst region
    GUO Shuang-yan, YOU Qian, SHU Wen, ZHANG Jun-chen, ZHANG Nan, PAN Chang-bin, PAN Zi-ping
    2026, 65(2):  57-62.  doi:10.14088/j.cnki.issn0439-8114.2026.02.009
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    Aiming at the problem of high selenium content but low bioavailability in selenium-rich soil in the Southwest karst region, a pot experiment was conducted with treatments of no lime application (CK) and five lime application gradients (T1~T5, adding 5, 10, 20, 30, 40 g lime per 9 kg soil, respectively) to explore the effects on soil pH, selenium speciation transformation, selenium availability, and selenium uptake and accumulation in sorghum. The results showed that lime application increased soil pH, promoted the transformation of organically combined selenium to water-soluble selenium and exchangeable selenium, and increased the content of available selenium in soil. Appropriate lime application significantly enhanced the accumulation levels of selenium in sorghum roots and grains, with the maximum increase reaching 78.57% and 67.19%, respectively. Correlation analysis indicated that the content of available selenium in soil was a key factor affecting selenium uptake in sorghum, showing a strong positive correlation with selenium content in roots and grains. The selenium content in roots had a strong negative correlation with the content of organically combined selenium, while the selenium content in grains had a weak correlation with organically combined selenium. In the tested acidic selenium-rich soil, applying 20~30 g lime (per 9 kg soil) effectively improved the bioavailability of selenium and promoted selenium accumulation in crops, and in practical application, this dosage range could be referred to with adaptive adjustments based on the specific acidic characteristics of the soil.
    Effects of nitrogen fertilizer form on yield, quality and nitrogen use efficiency of cigar filler tobacco
    LI Chuan-zong, CHANG Chen-ke, WU Zhe-kuan, YU Zhen, LIAO Shi-peng, RAO Xiong-fei
    2026, 65(2):  63-69.  doi:10.14088/j.cnki.issn0439-8114.2026.02.010
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    To clarify the regulatory effects of nitrogen fertilizer form on the yield and quality of cigar filler, a field experiment was conducted using the cigar filler variety Chuxue 14 (CX-14) to investigate the effects of ammonium nitrogen (NH4+), nitrate nitrogen (NO3-), and their mixed ratios on agronomic traits, yield benefits, chemical components, and sensory quality. The results showed that the mixed application of ammonium and nitrate nitrogen most significantly promoted plant height, stem girth, and leaf number of CX-14 during the later growth stage. All nitrogen application treatments significantly increased the yield and output value of both the cigar binder and filler, with the ammonium-to-nitrate ratio of 3∶1 treatment being the most effective. Nitrogen application increased the contents of nicotine, potassium, and nitrogen, while significantly decreasing the contents of reducing sugar, total sugar, and chlorine. The ammonium-to-nitrate ratio of 3∶1 treatment resulted in the highest nitrogen agronomic efficiency, nitrogen uptake efficiency, and dry matter mass. The ammonium-to-nitrate ratio of 3∶1 treatment achieved the highest overall sensory score (80 points), with significant improvements in aroma nuance and smoothness. This treatment synergistically optimized carbon and nitrogen metabolism and ion absorption, thereby enhancing the agronomic traits, yield, quality, and nitrogen use efficiency of cigar filler tobacco, and comprehensively improving the sensory quality of cigar tobacco leaves.
    Spatial and temporal distribution characteristics of surface water body variation in Ningxia from 1990 to 2020
    ZHANG Yi-xian
    2026, 65(2):  70-80.  doi:10.14088/j.cnki.issn0439-8114.2026.02.011
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    In order to understand the spatial distribution characteristics of surface water resources in Ningxia and provide reference for the rational utilization of water resources in the region, based on the Joint Research Centre ( JRC ) global surface water data set, statistical analysis, Mann-Kendall method, wavelet analysis and other methods were used to study the variation characteristics, mutation characteristics and oscillation period of surface water in Ningxia from 1990 to 2019, and the spatial evolution characteristics of water density in the region from 1990 to 2020 were also analyzed. The results showed that the surface water area in Ningxia increased by 141.28 km2 from 1990 to 2019, with an increase of 188.3%, and the number of water bodies increased by 1 861, with an increase of 42.0%. The total area and number of water bodies showed a decreasing trend from 1990 to 1999, the area of water bodies increased significantly after 2006, the number of water bodies increased significantly after 2016, and the number of water bodies increased abruptly in 2014. The total area and total number of water bodies mainly had the first oscillation main period of 22 years and the second oscillation main period of 30 years. From the perspective of spatial density of water bodies, the fastest growing area of water bodies in Ningxia was along the Yellow River, the largest area of density increase was the Yinbei Plain Irrigation District, and the density of water bodies in the central and southern regions had expanded in space.
    Effects of ecological products on people’s well-being in the Yi River Basin
    WU Cheng-zhou, WEI He-jie, LI Hai-wei, WANG Yu-han, CAI En-xiang
    2026, 65(2):  81-88.  doi:10.14088/j.cnki.issn0439-8114.2026.02.012
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    To explore the correlation characteristics and impact mechanisms between ecological products and people’s well-being in the Yi River Basin, this study took Luanchuan County, Song County, Yichuan County, and Yanshi District from the upper to lower reaches of the Yi River Basin as the study area. Field investigations and questionnaire surveys were conducted among local residents, with 400 valid questionnaires collected. On this basis, an evaluation index system for people’s well-being was constructed using the analytic hierarchy process (AHP), and Spearman’s rank correlation coefficient and radar charts were employed to analyze the correlation characteristics between the importance of ecological products and people’s well-being. The results showed that residents in the Yi River Basin recognized the importance of ecological products in the following descending order: agricultural products and water resources, regulating service products, cultural service products, and forestry products. The level of people’s well-being from high to low was Yanshi District, Luanchuan County, Yichuan County, and Song County, with factors such as housing, daily necessities, health status, and medical services having a greater impact on people’s well-being. People’s well-being was most closely related to water resources, followed by ecological regulating service products and agricultural products. The ecological products in the Yi River Basin were closely related to people’s well-being. Measures such as enhancing people’s awareness of the importance of ecological products, exploring effective pathways for realizing the value of ecological products, and establishing downstream-to-upstream ecological compensation mechanisms could not only contribute to the protection and development of ecological products but also promote the synergistic enhancement of ecological benefits and people’s well-being.
    Land use dynamics and landscape pattern evolution in the Dongjiang River source area from 2000 to 2020
    SHI Xiao-wen, WANG Yuan-dong, MING Le, MENG Li-hong
    2026, 65(2):  89-94.  doi:10.14088/j.cnki.issn0439-8114.2026.02.013
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    To reveal the response mechanisms of land use and landscape pattern to the dual pressures of rapid urbanization and ecological protection in the Dongjiang River source area, based on Landsat remote sensing images from 2000, 2010, and 2020, this study quantitatively analyzed the characteristics of land use change and the evolution of the landscape pattern in the Dongjiang River source area by using the transfer matrix and dynamic degree model. The results showed that significant changes occurred in land use types in the study area from 2000 to 2020. The conversion between cultivated land and forest land was the most prominent, while the degree of conversion among other land use types was lower, though certain changing trends were still observed.Cultivated land and forest land were the dominant land use types. The rate of land use change showed an upward trend from 2000 to 2020, and the comprehensive land use dynamic degree increased from 0.14%/year during 2000-2010 to 0.39%/year during 2010-2020, a rise of 178.6%, indicating that the regional land system transitioned from a phase of “slow adjustment” to one of “accelerated restructuring”. At the landscape level, the evolution of the landscape pattern was characterized by an increase in patch density and landscape fragmentation, a rise in landscape diversity with a trend towards more balanced spatial distribution, and a decrease in landscape connectivity leading towards differentiation.At the land use type level, the fragmentation of cultivated land and forest land was prominent, the distribution and shape of cultivated land became more regular, the forest land landscape structure evolved from relatively intact to fragmented and artificial, grassland fragmentation intensified, and construction land expansion was obvious with simplified morphology.
    Plant Protection
    The biocontrol efficacy of two Bacillus strains against tobacco bacterial wilt
    CHENG Wei, WANG Liao-yuan, HUO Guang, KONG Wei, SHI He-li, XIANG Bi-kun, ZHANG Ran, DONG Rui-jie, YANG Zhao-yu, CAO Yuan-yuan, TAN Jun, WANG Rui
    2026, 65(2):  95-100.  doi:10.14088/j.cnki.issn0439-8114.2026.02.014
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    To evaluate the biocontrol efficacy of Bacillus atrophaeus strain BS17 and Bacillus velezensis strain BS22 against tobacco bacterial wilt and determine the optimal application timing, LIVE/DEAD fluorescence staining and scanning electron microscopy were employed to observe the inhibitory effects of these two strains on Ralstonia solanacearum (the causal agent of bacterial wilt) and analyze their inhibitory mechanisms. Additionally, pot experiments were conducted with bacterial suspensions applied at three key growth stages: Sowing stage, seedling emergence stage, and transplanting stage. The results demonstrated that both strains primarily inhibit Ralstonia solanacearum by disrupting the integrity of the bacterial cell membrane. The BS22 strain exhibited superior control efficacy when applied at the sowing stage (98.23%), significantly higher than that of BS17 (79.64%, P<0.001). When applied at the seedling emergence stage, no significant difference in control efficacy was observed between the two strains (BS17: 88.49%, BS22: 85.84%). However, when applied at the transplanting stage, both strains exhibited significantly reduced control efficacy (BS17: 3.54%, BS22: 48.67%). The BS22 strain demonstrated superior biocontrol efficacy against tobacco bacterial wilt compared to the BS17 strain, particularly when applied at the sowing stage.
    Exogenous dsRNA-mediated RNA interference suppresses potato virus Y (PVY) infection in tobacco by targeting and silencing the CP gene
    XU Ru-bing, LUO Zhao-peng, LI Yan-yan, LIU Jun-xia, YANG Jun, QIN Yao
    2026, 65(2):  101-106.  doi:10.14088/j.cnki.issn0439-8114.2026.02.015
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    To evaluate the inhibitory effect of exogenous double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) on potato virus Y (PVY) infection in tobacco, the coat protein (CP) gene of PVY was cloned. Subsequently, a dsRNA fragment targeting this gene was designed based on sequence analysis. A 474-bp dsRNA targeting the PVY CP gene was synthesized in vitro and applied together with PVY onto tobacco plants. The relative mRNA level of the CP gene in the inoculated leaves was determined by quantitative real-time PCR (qRT-PCR), while viral symptom development was observed over time. Both the disease incidence and disease index were recorded to comprehensively evaluate the control efficacy of the dsRNA treatment against PVY. The results showed that exogenous application of PVY CP gene targeting dsRNA significantly suppressed the expression of the PVY CP gene in the inoculated leaves and effectively alleviated virus-induced symptoms, resulting in a control efficacy of 50.58% in pot trials.
    Horticulture & Local Products
    Applicability evaluation of WOFOST model for flue-cured tobacco growth simulation in Henan
    WANG Chen, LI Wen-feng, WANG Wei-min
    2026, 65(2):  107-112.  doi:10.14088/j.cnki.issn0439-8114.2026.02.016
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    In order to clarify the applicability of the WOFOST model to Henan flue-cured tobacco, sensitivity analysis of model parameters was conducted by OTA method based on field experimental data, meteorological data, and agrometeorological observation data from 2013 to 2024. The trial and error method was used in combination with experimental data to adjust model parameters and evaluate the applicability of the model to the growth and development of Henan tobacco. The results showed that SPAN, TSUM1, SLATB0.0, DTSMTB30, EEFTB40, TMPFTB20, AMAXTB0.0, CVL and RMS were identified as highly sensitive parameters through sensitivity analysis. Using the debugged model to simulate and verify the growth period and biomass of flue-cured tobacco, the R2 values for the bud stage and mature stage of tobacco were 0.89 and 0.83, RMSE were 3.4 and 2.4 days, and NRMSE were 1.8% and 0.9%, respectively. The RMSE of the model for simulating the leaf dry weight, stem dry weight, and leaf area index of tobacco were 198.0 kg/hm2, 220.4 kg/hm2, and 0.2 m2/m2, and the NRMSE were 11.9%, 17.4%, and 11.8%, respectively. The adjusted model performed well in simulating the growth and development process of tobacco leaves. By comparing the simulated yields determined by four methods, it was found that calculating the average of daily leaf dry weight data for the first 60 days of maturity through the model had the best fitting effect as the simulated yield, with a simulation accuracy of 89.5%, RMSE of 326.1 kg/hm2, and NRMSE of 10.7%. The model, after parameter debugging, could accurately simulate the changes in the growth process of Henan tobacco, providing a scientific management basis for actual production.
    Physicochemical characterization of nano-selenium synthesized by Bacillus and its application in selenium-enriched tomato cultivation
    MA Di, SHENG Qi, KAN Guo-shi, CHEN Hong-man
    2026, 65(2):  113-119.  doi:10.14088/j.cnki.issn0439-8114.2026.02.017
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    A SeNPs-synthesizing Bacterium MC-3 was isolated from serenium-rich soil and identified through morphological observation and 16S rDNA sequencing analysis. The physicochemical characteristics of SeNPs synthesized by strain MC-3 were analyzed by scanning electron microscope(SEM), energy dispersive spectrometer(EDS), X-ray diffraction(XRD), and Fourier transform infrared spectroscopy(FTIR), and SeNPs were applied to the cultivation experiment of selenium-enriched tomatoes. The results showed that strain MC-3 was Bacillus cereus. The SeNPs from reduction of Na2SeO3 by B. cereus MC-3 presented both crystalline and amorphous selenium nanoparticles, with a diameter range of 100~300 nm, and their molecule contain elements such as C, O, and Se, as well as functional groups such as -OH, C=O, and C-O, which were related to the stability of SeNPs. The SeNPs synthesized by B. cereus MC-3 were used as foliar fertilizer in the cultivation of selenium-enriched tomatoes. When the concentration of SeNPs was 70 mg/L, the selenium content in tomato fruits could reach 0.090 mg/kg FW. Meanwhile,the contents of soluble protein and vitamin C in the fruits were 41.25% and 35.00% higher than those in the control group, respectively. In conclusion, the appropriate concentration of SeNPs could be sprayed as foliar fertilizer for selenium-enriched tomato cultivation, and could improve the quality of tomato fruit.
    Biological characteristics of Phytophthora nicotianae resistant mutants to mandipropamid
    PENG Yu, CHENG Jia-wen, LI Cai-bin, MA Qian, MA Yu-xi, HE Yi, WU Xue-qiao, ZHOU Wen-liang, DENG Jian-qiang, QI Yong-xia
    2026, 65(2):  120-125.  doi:10.14088/j.cnki.issn0439-8114.2026.02.018
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    In order to clarify the biological characteristics, genetic stability of drug resistance, and pathogenicity of mandipropamid-resistant strains of Phytophthora nicotianae, the P. nicotianae strain YCHJ was used as the tested strain. Mandipropamid-resistant strains of P. nicotianae were obtained by UV-irradiating. The biological characteristics of mutant strains were compared with those of their parent strain such as colony morphology, hyphal growth rate, zoosporangia morphology and production, and germination ability of zoospores. The genetic stability and pathogenicity of the mutant strains were assessed by the mycelial growth rate method and greenhouse pot trials, respectively. The results showed that the resistance levels of the mutant strains YCHJ2-12, YCHJ5-7 and YCHJ5-16 induced by mycelial UV-irradiating were 62.99, 47.06 and 10.11, respectively. The resistance levels of the mutant strains YCHJZ2-2 and YCHJZ4-13 induced by UV-irradiating of zoospores were 54.79 and 17.75, respectively. The results of biological characteristics showed that there was no significant difference in hyphal morphology and zoosporangia morphology between the mutant strains and the parent strain, but there were differences in the mycelial growth rate, the size of zoosporangia and the height of papillae. The average mycelial growth rate of YCHJ2-12 and YCHJZ2-2 was significantly higher than that of the parental strain YCHJ. The length, width and papillae height of the zoosporangia produced by YCHJZ4-13 were significantly higher than those of YCHJ. In addition, the zoosporangia production of YCHJZ4-13, YCHJ5-16 and YCHJZ2-2 was significantly higher than that of YCHJ, but the germination rate of zoospores and pathogenicity of the mutant strains were lower than those of YCHJ. The resistance of YCHJZ4-13 and YCHJ5-16 to mandipropamid couldn’t be stably inherited, and the resistance of other strains decreased with the increase of cultivation generations. Based on these, it was speculated that the resistance risk of P. nicotianae to mandipropamid was low to moderate.
    Evaluation of salt tolerance and physiological response mechanisms in Paeonia lactiflora
    BA Xiao-jian, MA Yu, ZHU Qing-yi, XIA Mei, LU Xiao-yan
    2026, 65(2):  126-132.  doi:10.14088/j.cnki.issn0439-8114.2026.02.019
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    Taking two herbaceous peony (Paeonia lactiflora Pall.) cultivars, Lan Fushi (L) and Da Fugui (D), as test materials, a pot experiment was conducted to investigate the growth and physiological changes of herbaceous peony under five NaCl concentration treatments (0, 100, 200, 300, 400 mmol/L). The results showed that the salt tolerance of Lan Fushi (L) was stronger than that of Da Fugui (D). With the increase of NaCl concentration, the phenotypic score, leaf relative electrical conductivity, soluble protein content, soluble sugar content, malondialdehyde (MDA) content and peroxidase (POD) activity of both cultivars increased significantly, while the leaf thickness, net photosynthetic rate (Pn) and maximum photochemical quantum yield (Fv/Fm) decreased significantly. Correlation analysis indicated that the salt-tolerance phenotypic score was significantly negatively correlated with plant height (P<0.05), and extremely significantly negatively correlated with leaf thickness, SPAD value, Pn, Fv/Fm and leaf relative water content (P<0.01). Meanwhile, it was extremely significantly positively correlated with leaf relative electrical conductivity, soluble protein content, soluble sugar content, catalase (CAT) activity, POD activity and MDA content (P<0.01), but had no significant correlation with superoxide dismutase (SOD) activity (P>0.05). Furthermore, stepwise regression analysis was used to establish the optimal linear regression equation: Salt-tolerance phenotypic score = 4.198-3.747 × Fv/Fm, and Fv/Fm was identified as an evaluation index for salt tolerance of herbaceous peony.
    Influencing factors and anatomical structure characteristics of rooting in cuttings of Rhododendron mucronatum and Rhododendron obtusum Dayuanyangjin
    ZHENG Yong-run, LIU Xiu-qun
    2026, 65(2):  133-142.  doi:10.14088/j.cnki.issn0439-8114.2026.02.020
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    To investigate the optimal conditions for the rooting of Rhododendron cuttings and their anatomical structure characteristics, semi-lignified current-year branches of Rhododendron mucronatum and Rhododendron obtusum Dayuanyangjin were used as materials. The influencing factors of cutting rooting were analyzed from substrate types, plant growth regulator types and concentrations. Additionally, sectioning techniques were employed to observe anatomical structural changes during the rooting process. The results showed that the optimal external conditions for rooting in Rhododendron mucronatum were a peat∶perlite mixture(3∶1,V/V, the same below) supplemented with 800 mg/L α-naphthaleneacetic acid(NAA). For Rhododendron obtusum Dayuanyangjin, the optimal treatment was a peat∶perlite mixture(3∶1) supplemented with 400 mg/L indole-3-butyric acid(IBA)+NAA in a 1∶1 ratio(V/V). During the rooting process of Rhododendron mucronatum and Rhododendron obtusum Dayuanyangjin, the occurrence method of adventitious roots belonged to the induced root primordium rooting type, and the occurrence sites were both located in the cambium, belonging to the cortex rooting type.
    Medicinal Plant
    Anti-inflammatory activity of different polar fractions from Jasminum nervosum Lour
    GAO Mei-fei, ZHOU Jia-jing, YUAN Cai-ying, PAN Xiao-fang, HUO Li-ni, CHEN Rui
    2026, 65(2):  143-147.  doi:10.14088/j.cnki.issn0439-8114.2026.02.021
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    Models of xylene-induced ear edema, carrageenan-induced paw edema, and cotton pellet-induced granuloma in mice were established to investigate the effects of petroleum ether, ethyl acetate, n-butanol, and aqueous fractions of Jasminum nervosum on acute and chronic inflammation. Serum levels of inflammatory factors were measured, and histopathological changes were observed. Acute toxicity tests indicated that the ethanol extract of Jasminum nervosum exhibited no significant toxicity. In anti-inflammatory assays, compared with the model group, the dexamethasone group and the high-dose groups of the petroleum ether and ethyl acetate fractions significantly inhibited mouse ear edema (P<0.01), and the low-dose ethyl acetate fraction group also showed significant inhibition (P<0.05). In the paw edema model, the high- and medium-dose ethyl acetate fraction groups significantly suppressed edema (P<0.01, P<0.05). In the granuloma model, the high-dose ethyl acetate fraction group significantly reduced granulation tissue hyperplasia (P<0.01). Additionally, all dose groups of the ethyl acetate fraction significantly lowered serum levels of NO, iNOS, IL-6, IL-1β, and COX-2 (P<0.01), and the high-dose group alleviated inflammatory cell infiltration in paw tissue. Different polar fractions of Jasminum nervosum exhibited anti-inflammatory effects, with the ethyl acetate fraction demonstrating the most significant inhibition of both acute and chronic inflammation. Its mechanism was likely related to the regulation of inflammatory factors such as NO, iNOS, IL-6, IL-1β, and COX-2.
    Comparative transcriptome analysis of the hook and inflorescence across different developmental stages in Uncaria rhynchophylla
    SANG Si-hong, LI Tao, CHEN Hong-yu, REN De-qiang, LUO Qiu-mei, YAN Fu-lin
    2026, 65(2):  148-155.  doi:10.14088/j.cnki.issn0439-8114.2026.02.022
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    The hooks and inflorescences of Uncaria rhynchophylla, two homologous organs at different developmental stages, including hook buds and inflorescence buds at the initial stage, as well as formed hooks and young inflorescences, served as materials for transcriptome sequencing. Differentially expressed genes (DEGs) in the hook developmental process (hook buds vs. hooks) and the inflorescence developmental process (inflorescence buds vs. young inflorescences) were screened, and the transcriptional patterns of the two developmental processes were compared through cluster analysis. The results showed that a total of 97 060 unigenes were obtained from RNA-seq data assembly. In total, 1 637 and 6 179 DEGs were identified in the hook and inflorescence developmental processes, respectively. Among these, genes with significant differential expression (|log2FC)| ≥ 2) exhibited distinct differences in GO functional clustering and KEGG pathway enrichment. There were 524 common DEGs shared by the two developmental processes; enrichment analysis indicated that both hook and inflorescence development involved responses to endogenous signals and external environmental stimuli. Further screening of these common DEGs identified 52 transcription factor (TF) genes, which were clustered into 7 categories based on their expression patterns. The majority of these genes were involved in regulating reproductive development, suggesting that the transcriptional regulatory network they constituted was closely related to the divergence in developmental fate between hooks and inflorescences.
    The identification, biological characteristics, and artificial cultivation of a wild Ganoderma gibbosum
    SUN Ke-ke, WU Xi-yuan, YAO Tai-mei, ZHENG Zhi-xin, YAN Hai-yan, FENG Sha-sha, LAN Jin-ping, XU Yan-yan
    2026, 65(2):  156-161.  doi:10.14088/j.cnki.issn0439-8114.2026.02.023
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    A wild macrofungus collected from a camphor tree in Jiaxing City, Zhejiang Province, was used as the experimental material. It was identified through a comprehensive approach involving morphological observation and ITS (Internal transcribed spacer) sequence analysis, and its biological characteristics were determined, along with trials on artificial domestication and cultivation. The results indicated that this wild fungus was Ganoderma gibbosum, with its hyphae exhibiting distinct clamp connections. The optimum temperature for hyphal growth was 30 ℃, and the optimal pH was 6. Among the tested cultivation formulas, the one consisting of 50% sawdust, 38% cottonseed hulls, 10% wheat bran, 1% gypsum, and 1% lime (Formula 1) resulted in the fastest hyphal growth, the most vigorous growth, and successful production of fruiting bodies.
    Animal Husbandry & Veterinary Medicine
    Association analysis between BMP4, Krt85, and PPARGC1A gene polymorphism and meat quality traits in Small Tail Han sheep
    XU Tie-shan, DU Qiu-ming, HAN Zhi-song, HAO Xiao-ping, CHEN Jian, BAI Jun-yan
    2026, 65(2):  162-166.  doi:10.14088/j.cnki.issn0439-8114.2026.02.024
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    InDel polymorphism was used to analyze the four polymorphic sites of the three candidate genes of BMP4, Krt85 and PPARGC1A genes (including drip loss, shear force, moisture, cooking loss, color, crude protein, and crude fat), so as to provide some scientific basis for improving the meat quality of the Small Tail Han sheep. The results showed that: ① polymorphisms in the four sites of the three genes were detected, and all four polymorphic sites had genotypes DD, ID and II. ② The polymorphic information content of the BMP4 gene was 0.371, and was moderate polymorphism in the population(0.25≤PIC<0.50); the polymorphic information content of the Krt85-1 gene locus was 0.337, and was moderate polymorphism in the population(0.25≤PIC<0.50); the polymorphic information content of the Krt85-2 gene locus was 0.375, and was moderate polymorphism in the population(0.25≤PIC<0.50); The polymorphic information content of the PPARGC1A gene was 0.294, and was moderate polymorphism in the population(0.25≤PIC<0.50). ③ In terms of cooking loss, the ID genotype of BMP4 gene was significantly lower than that of DD genotype (P<0.05). In terms of shear force, the DD genotype of PPARGC1A gene was significantly lower than that of ID and II (P<0.05). In terms of color, the II genotype of the Krt85-1 gene locus was significantly lower than the DD and ID genotypes (P<0.05). For crude fat, the ID genotype at the Krt85-2 locus was significantly lower than that of genotype II (P<0.05). In conclusion, the BMP4, Krt85, and PPARGC1A genes could all be used as candidate genes for improving the meat quality of Small Tail Han sheep.
    Dynamics of waterfowl community in Chenhu Wetland Nature Reserve in Wuhan City from 2005 to 2021
    XIE Hong, XU Yong-rong, YAN Jun, WANG Kai-xuan, MING Liang, LIN Ma-yong, LI Peng, XIAO Zhi-yan
    2026, 65(2):  167-175.  doi:10.14088/j.cnki.issn0439-8114.2026.02.025
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    Based on long-term fixed-point observation data, the long-term dynamics of the waterbird community since the establishment of the Chenhu Wetland Nature Reserve were analyzed. It was found that the number of species and individuals in the Chenhu wetland waterbird community increased significantly from 2005 to 2021, with a fluctuating increase in species diversity indices. Winter migratory birds constituted the majority of the community, with waders predominating in terms of species count and swimming birds in terms of individual count. The species count of omnivorous waterbirds was dominant, and the composition of individual count had shifted from having no clear dominant type to a predominance of herbivores. The Anseriformes and Charadriiformes were the dominant orders in the waterbird community. The number of dominant species in the waterbird community gradually decreased after 2015. The waterbird community underwent two stages: a period of significant changes in species composition and a period of minor changes in species composition. Endangered and rare waterbird species were observed annually in Chenhu Wetland from 2005 to 2021. After 2017, the number of endangered and rare waterbird species began to steadily increase, and unique waterbird species were observed annually after 2018.
    Detection Analysis
    GC-MS analysis of the waxy metabolites of Corydalis saxicola
    ZHANG Yi-xuan, HUANG Yan-qi, LEI Ming, WEI Ying, XIAO Dong, LI Cui
    2026, 65(2):  176-182.  doi:10.14088/j.cnki.issn0439-8114.2026.02.026
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    Taking the leaves of Corydalis saxicola as the research object, the waxy metabolites of Corydalis saxicola were analyzed and identified by gas chromatography-mass spectrography(GC-MS). The results showed that there were significant differences in the composition of waxy metabolites of Corydalis saxicola in the five producing areas. A total of several key differential metabolites were identified, including long-chain alkanes (such as n-29-alkane and n-54-alkane), ketones, sterols (such as γ-oryzanol), and alkaloids (such as tetrahydroberberine). Pathway enrichment analysis revealed that the differential metabolites were significantly enriched in metabolic pathways such as "isoquinoline alkaloid biosynthesis" and "biosynthesis of various secondary metabolites". In addition, bioactive defensive compounds such as podophyllotoxin were also found in the wax. The results provided a scientific basis for quality evaluation and related product development of Corydalis saxicola.
    Optimization of near-infrared spectral wavelength variables for vine tea-flavored beer based on the SPA algorithm
    GUO Yi-feng
    2026, 65(2):  183-187.  doi:10.14088/j.cnki.issn0439-8114.2026.02.027
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    The wavelength variable selection method for infrared spectroscopy primarily employed multiple iterations to screen wavelength variables with significant model contribution from the full-spectrum data. This approach exhibited poor resistance to redundant information interference, making it difficult to accurately reflect the true characteristics of the sample. To address this, a wavelength variable selection method based on the SPA algorithm was proposed for near-infrared spectroscopy of vine tea-flavored beer. Multiscattering correction was applied to the near-infrared spectral data to enhance absorption information correlated with component content and reduce noise. The continuous projection algorithm was used for preliminary wavelength screening. Combined with correlation and collinearity penalties for the target components in wisteria tea flavor detection, this approach selected a wavelength subset with low information redundancy and strong predictive capability. Combined entropy and mutual information were used to assess wavelength redundancy, constructing a scoring function incorporating information content and redundancy penalties to select the subset with the highest overall score. The results showed that after wavelength variable optimization using this method, spectral absorption coverage remained stable at approximately 95%, indicating highly satisfactory optimization outcomes.
    Effect of shaking-green process on quality and aroma characteristics of Xinyang black tea
    YI Chao, JIANG Shuang-feng, ZHENG Jie, ZHU De-yan, ZHAO Si
    2026, 65(2):  188-194.  doi:10.14088/j.cnki.issn0439-8114.2026.02.028
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    To investigate the regulatory effect of the shaking-green process on the quality of Xinyang black tea, fresh leaves of Wuniuzao and Xinyang population cultivar tea plants were used as raw materials to prepare black tea using traditional process and shaking-green process, respectively. Differences in quality and aroma components were systematically analyzed by combining sensory evaluation, physicochemical component determination, and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology. The results showed that the shaking-green process increased the contents of water extract, soluble sugar, gallocatechin, and theaflavins in Xinyang black tea, effectively reducing the bitter and astringent taste of the tea infusion and making the flavor more mellow and rich. A total of 50 aroma components were identified by GC-MS technology. In the black tea made from Xinyang population cultivar, the shaking-green process increased the contents of aldehydes, alkenes, and esters. In the black tea made from Wuniuzao, the shaking-green process increased the contents of aldehydes, alkenes, ketones, alcohols, and furans.Among the four tea samples, the black tea made from Xinyang population cultivar using the shaking-green process had relatively higher contents of linalool, methyl salicylate, trans-2-hexenyl hexanoate, and linalool oxide. The black tea made from Wuniuzao using the shaking-green process had relatively higher contents of linalool, (Z)-3-hexenyl hexanoate, methyl salicylate, and linalool oxide. The black tea made from Xinyang population cultivar using the traditional process had relatively higher contents of linalool, methyl salicylate, trans-2-hexenyl hexanoate, linalool oxide, and phenylacetaldehyde. The black tea made from Wuniuzao using the traditional process had relatively higher contents of linalool, (Z)-3-hexenyl hexanoate, methyl salicylate, trans-2-hexenyl hexanoate, and linalool oxide.In conclusion, introducing the shaking-green process during the processing of Xinyang black tea could effectively reduce the bitter and astringent taste, enhance the mellow sensation, and improve the aroma quality.
    Information Engineering
    3D indoor semantic scene completion method based on multi-channel difference fusion
    WANG Chang-shuan, LU Yun-he, JIANG Jian-wu
    2026, 65(2):  195-201.  doi:10.14088/j.cnki.issn0439-8114.2026.02.029
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    To address the issues of missing 3D perceptual information and insufficient semantic understanding caused by object occlusion and compact spatial structures in complex indoor environments, a multi-channel difference fusion network for semantic scene completion (MCDFNet) based on RGB-D input was proposed. The model designed a multi-channel difference fusion (MCDF) module, which, based on unified RGB-D representation, extracted differential features among RGB, Depth, and their fused channels to effectively enhance the modeling capability for the geometric structure and semantic consistency of occluded regions. Experiments on the NYUCAD dataset showed that the MCDFNet model achieved an accuracy of 72.8%, a precision of 77.1%, and a mean Intersection over Union (mIoU) of 43.4% while maintaining a single-scene completion inference time of 1.9 s, outperforming mainstream models such as AICNet, DDRNet, and GRFNet. Ablation studies demonstrated that introducing the MCDF module could improve the mIoU by 1.5 percentage points, proving its critical role in enhancing completion accuracy. The model could operate stably in highly occluded indoor environments, improving the completeness and practical value of 3D maps, and was suitable for various typical indoor application scenarios.
    A non-contact estimation method for feed residue based on dual-modal MobileViTv2
    CAI Xiao-jin, BAI Tao, LI Xiang, QIAO Rui-qiang
    2026, 65(2):  202-208.  doi:10.14088/j.cnki.issn0439-8114.2026.02.030
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    Aiming at the problems of traditional feed residue detection methods relying on contact sensors, high cost, and the need to modify feeding troughs, a lightweight convolutional fusion regression model (dual-modal MobileViTv2 + CMFIM + SE) based on dual-modal MobileViTv2 was proposed to achieve non-contact and high-precision automatic estimation of feed residue. Taking RGB images and depth images as input, the model extracted multi-scale features respectively through the dual-modal MobileViTv2 and introduced a cross-modal multi-scale feature interaction module (CMFIM) at four levels to achieve spatial-channel dual interaction between RGB and depth features. An SE module was employed to adaptively calibrate channel weights and enhance high-level semantic representation capability. The prediction results were output through a multilayer perceptron regression head. On the self-built dataset, the mean absolute error (MAE) and root mean square error (RMSE) of the dual-modal MobileViTv2 + CMFIM + SE model were 98.24 g and 140.21 g, respectively, which represented reductions of 21.65% and 16.73% compared to the dual-modal MobileViTv2 model without the CMFIM and SE modules, and the parameter size of the model was only 9.9×106. The model combined the advantages of high accuracy, strong robustness, and lightweight design, providing a feasible technical pathway for precision feeding in intelligent livestock farming.
    Biological Engineering
    Screening and functional validation of endogenous promoters from Bacillus amyloliquefaciens for fengycin synthesis
    ZHOU Lu, TIAN Yuan, YU Chong, XIA Hai-hua, YAN Geng-xuan
    2026, 65(2):  209-214.  doi:10.14088/j.cnki.issn0439-8114.2026.02.031
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    Using Bacillus amyloliquefaciens TF28 as the host, and by integrating genomic and transcriptomic data, 12 upstream endogenous promoters from highly expressed genes were screened. By constructing a GFP reporter system and evaluating the activity of each promoter in Bacillus, it was found that the promoter strengths of PspoVG, PqoxC, PqoxB, and Pgpd were higher than that of the commonly used promoter P43. When driven by the PspoVG promoter, the transcriptional level of the fenC gene was 4.62 times that of the P43 promoter, and the fengycin yield increased from 84.3 mg/L to 101.2 mg/L. Regarding promoter structure, the sequences of the -35 and -10 regions of PspoVG were highly consistent with the consensus sequences of typical strong promoters, which might be the structural basis for its high-efficiency transcriptional drive.
    Genome-wide identification and functional evolutionary characterization of the HSF gene family in Amorphophallus konjac
    CAI Yang-guang, LI Jian-kang, DUAN Long-fei, QIN Jian-feng, WU Jun-qi
    2026, 65(2):  215-222.  doi:10.14088/j.cnki.issn0439-8114.2026.02.032
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    To elucidate the molecular response mechanism of Amorphophallus konjac under high-temperature stress, based on the whole-genome data of Amorphophallus konjac, the heat shock transcription factor (HSF) gene family members were systematically identified using bioinformatics methods, and their physicochemical properties, evolutionary relationships, conserved motifs, and protein interaction networks were analyzed. The results showed that a total of 28 AkHSF gene family members were identified in the Amorphophallus konjac genome, which were unevenly distributed on 8 chromosomes. By constructing a phylogenetic tree, the HSF members of Amorphophallus konjac and Arabidopsis thaliana were co-classified into five subgroups, revealing the evolutionary conservation of the gene family. The analysis of conserved motifs and gene structures indicated that members within the same subgroup shared similar motif compositions, suggesting functional similarity, while differences existed among different subgroups, implying functional divergence. Protein interaction prediction revealed six interaction relationships among seven AkHSF members. The AkHSF gene family members exhibited both conservation and adaptive evolution in their functions related to heat stress response.
    Economy & Management
    Research on vocational education for Chinese farmers: Historical evolution, current development and future prospects
    ZHANG Shao-hua, ZHANG Luan, ZHANG Hui-jie
    2026, 65(2):  223-229.  doi:10.14088/j.cnki.issn0439-8114.2026.02.033
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    Anchored to the 2035 talent revitalization goal, the development trajectory of farmers′ vocational education was divided into three phases: history (1949-2020), present (2021-2025), and future (2026-2035). Based on the "demand-target-tool" analytical framework, the historical development process of vocational education for farmers in China, its existing shortcomings and gaps, current supporting policies and implementation issues, as well as future development trends and urgent needs were systematically reviewed. Some suggestions of enhancing support for agriculture-related vocational education and training across the entire industrial chain, continuously optimizing the allocation of vocational education resources in both spatial and target dimensions, establishing and improving a collaborative mechanism for vocational training programs featuring "top-level coordination and tiered implementation",and establishing and improving a performance evaluation system for agriculture-related vocational education funding that covered theoretical, practical, and applied dimensions were put forward.
    The empowerment of rural digital economy on the adoption of green agricultural technology:An analysis of the mediating effects based on service outsourcing and farmers′ trust
    CHEN Yi-qi, XIE Wei, LI Sha-sha
    2026, 65(2):  230-235.  doi:10.14088/j.cnki.issn0439-8114.2026.02.034
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    Based on survey data of farmers in Yunnan Province in 2023, the impact of the rural digital economy on farmers′ adoption of green agricultural technology was empirically analyzed and the mediating mechanisms of agricultural socialized services and farmers′ trust were explored. The results showed that the rural digital economy significantly promoted the adoption of green agricultural technologies by farmers, with a nonlinear characteristic of increasing marginal effects. Mechanistically, the digital economy enhanced adoption willingness by promoting the outsourcing of agricultural production services (e.g., unified pest control, water-saving irrigation) and strengthening farmers′ trust in technologies and service providers. Heterogeneity analysis indicated that this effect was more pronounced among large-scale, high-income, and new-generation farmers. Furthermore, policies such as the construction of high-standard farmland and green subsidies could strengthen the promoting effect of the digital economy. It was recommended to enhance rural digital infrastructure, innovate "digital + service" models, build trust-based promotion systems, and implement differentiated policies to advance agricultural green transformation.
    Rural Revitalization
    Research on the path of digital transformation in rural governance
    DONG Shi-juan
    2026, 65(2):  236-240.  doi:10.14088/j.cnki.issn0439-8114.2026.02.035
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    The digital transformation of rural governance was an inevitable choice in line with the development of rural revitalization in the new era. The internal driving force behind this transformation stemmed from the interactive logic among national strategic orientation, the penetration of digital technology, and the decline of traditional governance models. However, the transformation process faced three practical challenges: unequal distribution of digital resources leading to imbalance in governance participation, tension between the rationality of technological tools and local needs, and data security vulnerabilities and the sustainability of governance. To further advance the modernization of China’s rural governance system and governance capabilities, it was necessary to explore the digital transformation path of rural governance from the perspectives of technology, culture, and organization, based on the logic of the mutual construction of technology and society, in order to enhance the efficiency of rural governance and provide support for the systematic implementation of the rural revitalization strategy.
    Pathways of eco-technology empowering the construction of the “Green Villages” in Fujian Province
    LI Xiao-wen
    2026, 65(2):  241-246.  doi:10.14088/j.cnki.issn0439-8114.2026.02.036
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    As China’s first national pilot zone for ecological conservation, Fujian had initiated the brand building of “Green Villages” to explore practical pathways to empower rural ecological revitalization through ecological technology. The synergy mechanism of institutional design, technology adaptation, financial support, and digital governance of “Green Villages” was systematically analyzed, and achievements in environmental governance, industrial integration, and livelihood improvement were summarized. Deep challenges such as the lack of institutional coordination, technological supply-demand misalignment, and inadequate resource base were also identified. The findings indicated that strengthening legal and policy coordination mechanisms, establishing precision-oriented empowerment systems, and innovating market-driven investment and financing mechanisms were crucial to achieving the deep integration between ecological technology and rural development. The practice of rural ecological revitalization in Fujian Province provided a set of replicable and promotable rural ecological revitalization paradigms for similar areas.
    Practical forms and driving logic of rural revitalization in promoting common prosperity:A typological analysis based on multiple cases
    WU Er, XU Jia-jun, LIU Ti-feng
    2026, 65(2):  247-256.  doi:10.14088/j.cnki.issn0439-8114.2026.02.037
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    Based on institutional embeddedness and endogenous development theories, a two-dimensional analytical framework of “government empowerment-endogenous dynamism” was constructed. By assessing the relative strengths of these two dimensions, four practical models for rural revitalization promoting common prosperity were identified: industry-driven wealth, talent-driven efficiency, culture-driven empowerment, and ecology-driven equity. These corresponded to dimensions of material affluence, efficiency enhancement, spiritual enrichment, and equitable promotion, respectively. Field investigations of typical village practices revealed that combinations of external government empowerment and internal village dynamism jointly shaped differentiated local pathways. Furthermore, this framework elucidated the driving mechanisms of each practice type: Industry-driven wealth followed a material-oriented industrial enrichment logic; talent-driven efficiency adhered to an efficiency-oriented talent-led logic; culture-driven empowerment followed a spirit-oriented cultural empowerment logic; ecology-driven equity adhered to an equity-oriented ecological co-creation logic. This integrated comparative framework provided a comparable theoretical perspective and concrete operational pathways for systematically understanding the diverse paths of rural revitalization promoting common prosperity. This study had certain limitations, and future research should further refine the theoretical framework to enhance its explanatory breadth and applicability.