植物促生菌DM在胁迫条件下产多胺成分的变化

doi:10.14088/j.cnki.issn0439-8114.2019.13.009

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (13): 44-47.doi: 10.14088/j.cnki.issn0439-8114.2019.13.009

植物促生菌DM在胁迫条件下产多胺成分的变化

刘秀, 郑宜令, 李艳秋, 范亚军

长春师范大学生命科学学院,长春 130032

收稿日期:2018-10-20 出版日期:2019-07-10 发布日期:2019-12-06
通讯作者: 范亚军（1977-）,女,副教授,博士,主要从事分子生物学研究,（电子信箱）xiaofan0524@126.com。
作者简介:刘秀（1994-）,男,吉林长春人,在读硕士研究生,研究方向为分子生物学,（电话）13843029841（电子信箱）13843029841@163.com。
基金资助:
国家自然科学基金项目（31600364）; 吉林省大学生创新创业项目（2018099）

Changes of polyamines produced by PGPR strains DM under stress conditions

LIU Xiu, ZHENG Yi-ling, LI Yan-qiu, FAN Ya-jun

Department of Biology,Changchun Normal University,Changchun 130032,China

Received:2018-10-20 Online:2019-07-10 Published:2019-12-06

摘要/Abstract

摘要： 利用不同pH及不同NaCl浓度的培养基培养植物促生菌阴沟肠杆菌（Enterobacter cloacae）菌株DM,高效液相色谱定性定量分析菌体上清液中多胺成分的变化。结果表明,加入NaCl胁迫后,腐胺、亚精胺浓度迅速升高,150 mmol/L NaCl培养液中腐胺、亚精胺、精胺浓度最高,随后降低;在pH 8时培养液中3种多胺含量最高,pH小于8 时溶液中多胺浓度随pH降低而减小,pH大于8 时溶液中多胺浓度随pH升高而减小。在盐胁迫及酸碱胁迫条件下,DM菌生物量下降,菌液中多胺浓度发生变化,提示DM菌可能通过调节多胺浓度来应对非生物胁迫。

关键词: 植物促生菌, 阴沟肠杆菌（Enterobacter cloacae）, 胁迫, 高效液相色谱, 多胺

Abstract: PGPR strains DM（Enterobacter cloacae） was cultured in the medium with different pH and NaCl concentration, and the polyamines in the supernatant of bacteria were qualitatively analyzed by high performance liquid chromatography （HPLC）. The results showed that the concentrations of putrescine, spermidine and spermidine were increased rapidly with NaCl adding, maximum value reached at the culture medium with 150 mmol/L NaCl, and then decreased. The concentration of three polyamines was the highest at the medium of pH 8, then the concentration of polyamines decreased when pH was less than 8, and also the concentration of polyamines decreased when pH was more than 8. The concentration of polyamines produced by DM bacteria changed under the salt, acid and alkali stress, this conclusion suggested that DM bacteria may respond to abiotic stress by regulating the concentration of polyamines.

Key words: plant growth-promoting bacteria PGPR, Enterobacter cloacae, stress, high performance liquid chromatography （HPLC）, polyamine

中图分类号:

Q936

刘秀, 郑宜令, 李艳秋, 范亚军. 植物促生菌DM在胁迫条件下产多胺成分的变化[J]. 湖北农业科学, 2019, 58(13): 44-47.

LIU Xiu, ZHENG Yi-ling, LI Yan-qiu, FAN Ya-jun. Changes of polyamines produced by PGPR strains DM under stress conditions[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(13): 44-47.

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植物促生菌DM在胁迫条件下产多胺成分的变化

Changes of polyamines produced by PGPR strains DM under stress conditions

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