湖北农业科学 ›› 2024, Vol. 63 ›› Issue (5): 126-135.doi: 10.14088/j.cnki.issn0439-8114.2024.05.023

• 畜牧·动科 • 上一篇    下一篇

黑麦草种子及幼苗对镉胁迫的生理响应

王建伟1a, 贺晓岚1b, 王根平2, 欧倩1b, 肖云娟1b, 杨雪丽1b   

  1. 1.凯里学院,a.理学院;b.大健康学院,贵州 凯里 556011;
    2.河北省农林科学院谷子研究所,石家庄 050035
  • 收稿日期:2022-10-09 出版日期:2024-05-25 发布日期:2024-06-04
  • 通讯作者: 贺晓岚(1980-),女,内蒙古乌兰察布人,副教授,博士,主要从事基因克隆及转基因研究,(电话)18285549260(电子信箱)helingzhi123@126.com。
  • 作者简介:王建伟(1982-),男,甘肃平凉人,教授,博士,主要从事植物营养与调控研究,(电话)18212333515(电子信箱)agan1982@126.com;
  • 基金资助:
    凯里学院博士专项(BS201506; BS201606); 贵州省科技计划项目(〔2017〕1167)

Physiological response of ryegrass seeds and seedlings to cadmium stress

WANG Jian-wei1a, HE Xiao-lan1b, WANG Gen-ping2, OU Qian1b, XIAO Yun-juan1b, YANG Xue-li1b   

  1. 1a. School of Science; 1b. School of Life and Health Science, Kaili University, Kaili 556011, Guizhou, China;
    2. Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, China
  • Received:2022-10-09 Published:2024-05-25 Online:2024-06-04

摘要: 为了探明不同黑麦草品种对镉(Cd)富集能力的差异及其生理机制,以6个黑麦草品种为材料,研究镉胁迫对其种子萌发、生物量、镉吸收、色素含量、光合特性及抗逆特性的影响。结果表明,与正常生长条件相比,镉胁迫对不同黑麦草品种种子萌发和根芽生长具有低促高抑作用。镉胁迫增加了黑麦草光合色素含量;镉胁迫增加了维纳斯、奔驰、维多利亚叶片的气孔导度、胞间CO2浓度、蒸腾速率;镉胁迫增加了奔驰、维多利亚、蓝标叶片的POD活性;镉胁迫增加了卡特、维多利亚叶片的SOD活性;镉胁迫增加了维多利亚、维纳斯叶片的CAT活性。奔驰、维多利亚、维纳斯可能较其他品种具有更强的耐镉性,从而减少镉对自身的危害;镉胁迫增加了所有品种可溶性糖含量及除卡特外的5个黑麦草品种脯氨酸含量,奔驰、蓝标、维多利亚能产生较多的渗透调节物质来减轻镉胁迫对其造成的伤害;在正常生长条件下,黑麦草地上部镉含量和镉累积量都较低,品种间差异较小。镉胁迫明显增加了黑麦草地上部镉含量和镉累积量,奔驰地上部镉含量和镉累积量最高,显著高于其他品种。综上,奔驰、维多利亚抗镉性及富镉能力较强,是镉污染土壤修复的优选品种。

关键词: 镉胁迫, 黑麦草(Lolium perenne L.), 种子萌发, 幼苗, 生理响应

Abstract: In order to explore the differences in cadmium (Cd) enrichment ability and physiological mechanisms among different ryegrass varieties, six ryegrass varieties were used as materials to study the effects of cadmium stress on seed germination, biomass, cadmium absorption, pigment content, photosynthetic characteristics, and stress resistance. The results showed that compared with normal growth conditions, cadmium stress had a low promoting and high inhibiting effect on seed germination and root and shoot growth of different ryegrass varieties. Cadmium stress increased the content of photosynthetic pigments in ryegrass;cadmium stress increased the stomatal conductance, intercellular CO2 concentration, and transpiration rate of Venus, Benz, and Victoria leaves;cadmium stress increased the POD activity of Benz, Victoria, and Blue Label leaves;cadmium stress increased the SOD activity of Carter and Victoria leaves; cadmium stress increased CAT activity in Victoria and Venus leaves. Benz, Victoria and Venus might have stronger cadmium tolerance than other varieties, thereby reducing the harm of cadmium to themselves;cadmium stress increased the soluble sugar content of all varieties and the proline content of five ryegrass varieties except Carter. Benz, Blue Label and Victoria could produce more osmotic regulatory substances to alleviate the damage caused by cadmium stress;under normal growth conditions, the cadmium content and accumulation in the upper part of rye grassland were relatively low, and the differences between different varieties were relatively small. Cadmium stress significantly increased the cadmium content and accumulation in the upper part of rye grassland, with Benz having the highest cadmium content and accumulation in the upper part, significantly higher than other varieties.In summary, Benz and Victoria had strong cadmium resistance and cadmium enrichment ability, making them the preferred varieties for cadmium contaminated soil remediation.

Key words: cadmium stress, ryegrass(Lolium perenne L.), seed germination, seedlings, physiological response

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