湖北农业科学 ›› 2023, Vol. 62 ›› Issue (12): 94-101.doi: 10.14088/j.cnki.issn0439-8114.2023.12.018

• 园艺 • 上一篇    下一篇

温度和氮营养盐对龙须菜生长与光合生理的影响

陈泽宇1, 丁雨豪2, 王静文1, 倪嘉璇1, 何昊林1, 徐加涛1, 蒋书英3, 王津果1   

  1. 1.江苏省海洋资源开发研究院/江苏省海洋生物产业技术协同创新中心/自然资源部滨海盐沼湿地生态与资源重点实验室/江苏海洋大学海洋科学与水产学院,江苏 连云港 222005;
    2.南京审计大学,南京 211815;
    3.连江罗源湾金牌渔业科技有限公司,福州 350512
  • 收稿日期:2022-10-08 发布日期:2024-01-10
  • 通讯作者: 王津果(1989-),女,河南许昌人,实验师,博士,主要从事海藻遗传育种的研究工作,(电话)15063131178(电子信箱)jgwang@jou.edu.cn。
  • 作者简介:陈泽宇(1998-),男,江苏南京人,在读硕士研究生,主要从事环境因素对大型经济海藻光合生理影响的研究工作,(电话)19502516858(电子信箱)531961601@qq.com。
  • 基金资助:
    田湾核电站温排水海域养殖综合开发利用研究项目资助项目(ASNJF20235-04); 福州市科技计划—农业科技项目(2020-N-17); 福建省科技特派员后补助项目; 江苏海洋大学人才引进科研基金资助项目(KQ19068); 江苏省海洋资源开发研究院开放基金课题(JSIMR202209); 江苏海洋大学研究生科研与实践创新计划项目(KYCX2022-04); 江苏省优势学科建设工程资助项目; 江苏省高等学校大学生创新创业训练计划资助项目(2020120068; 2020120170); 连云港市花果山英才计划资助项目(KK21054)

Effects of temperature and nitrogen nutrient on the growth and photophysiology of Gracilariopsis lemaneiformis

CHEN Ze-yu1, DING Yu-hao2, WANG Jing-wen1, NI Jia-xuan1, HE Hao-lin1, XU Jia-tao1, JIANG Shu-ying3, WANG Jin-guo1   

  1. 1. Jiangsu Institute of Marine Resources Development/Co-Innovation Center of Jiangsu Marine Bio-industry Technology/Key Laboratory of Coastal Salt Marsh Ecosystems and Resources, Ministry of Natural Resources/College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China;
    2. Nanjing Audit University, Nanjing 211815, China;
    3. Luoyuanwan Gold Medal Fishery Technology Co., Ltd., Fuzhou 350512, China
  • Received:2022-10-08 Published:2024-01-10

摘要: 以栽培龙须菜(Gracilariopsis lemaneiformis)为试验材料,设置2个温度水平(LT:20 ℃;HT:24 ℃)和3个氮营养盐浓度(LN:3.1 μmol/L;MN:30.1 μmol/L;HN:103.1 μmol/L),研究2个环境因子变化对龙须菜生长和光合生理的影响。结果表明,温度、氮营养盐及二者交互作用对龙须菜的相对生长速率(RGR)均具有显著影响(P<0.05),RGR随着温度的升高、氮营养盐浓度的增加而升高。叶绿素荧光参数分析显示,在同一氮营养盐浓度下,HT处理组的有效光合量子产量[Y(II)]在0.18~0.24内波动,均高于LT处理组,在同一温度培养条件下,HN处理组的Y(II)普遍高于LN处理组;温度对龙须菜的最大相对电子传递速率(rETRmax)存在显著影响(P<0.05);氮营养盐对不同处理下龙须菜的rETRmax、光能利用效率(α)、饱和光强(Ik)均存在显著影响(P<0.05),二者交互作用对α存在显著影响(P<0.05)。此外,温度、氮营养盐及二者交互作用对龙须菜的光合色素含量均具有显著影响(P<0.05);氮营养盐及二者交互作用对其可溶性蛋白质(SP)含量存在显著影响(P<0.05);在LT或HT条件下,氮营养盐浓度的增加显著促进叶绿素 a(Chl a)、类胡萝卜素(Car)和SP的合成(P<0.05)。HN和HT促进了龙须菜的生长,结果为解析龙须菜应对核电站温排水区的环境变化提供了理论数据。

关键词: 龙须菜(Gracilariopsis lemaneiformis), 温度, 氮营养盐, 生长, 光合生理

Abstract: The cultivated Gracilariopsis lemaneiformis was used as the experimental material, and two temperature levels(LT:20 ℃;HT:24 ℃) and three nitrogen nutrient concentrations(LN:3.1 μmol/L;MN:30.1 μmol/L;HN:103.1 μmol/L) were set to study the effects of temperature and nitrogen nutrient concentration changes on the growth and photophysiology of G. lemaneiformis. The results showed that temperature, nitrogen nutrient and their interaction had significant effects on the relative growth rate (RGR) of G. lemaneiformis P<0.05). The RGR increased with the temperature and nitrogen nutrient concentration increment separately. Further fluorescence parameters showed that the effective photosynthetic quantum yield [Y(II)] of HT treatment fluctuated in the range of 0.18~0.24, which was higher than that of LT treatment under the same nitrogen nutrient concentration and HN promoted the improvement of its Y(II)under the same temperature. Temperature had a significant effect on the maximum relative electron transfer rate (rETRmax, P<0.05), nitrogen nutrient had a significant effect on the rETRmax, light utilization efficiency (α) and saturation irradiance (Ik) (P<0.05)and their interaction had a significant effect on the α (P<0.05). Furthermore, temperature, nitrogen nutrient and their interaction had significant effects on the photosynthetic pigments contents of G. lemaneiformis P<0.05). Nitrogen and the interaction had significant effects on the SP content(P<0.05). Under LT or HT, the contents of chlorophyll a (Chl a), carotenoids (Car), and SP increased evidently with the increase of nitrogen nutrient concentration (P<0.05). Our findings suggested that HN and HT significantly promoted the growth of G. lemaneiformis, which provided the theoretical data for the cultivation of G. lemaneiformis in response to environmental changes in the thermal discharge area of Tianwan Nuclear Power Station.

Key words: Gracilariopsis lemaneiformis, temperature, nitrogen nutrient, growth, photophysiology

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