关键词: 子叶外植体, ALS抑制类除草剂, 西瓜自交系, 西瓜杂交种, 不定芽增殖, SU除草剂, IMI除草剂

Abstract: Using the cotyledons of watermelon inbred line D66 as explants, the explants were placed on a screening medium containing different concentrations of acetolactate synthase （ALS） inhibitor herbicides （SU herbicides, IMI herbicides）, and the optimal herbicide concentration was determined based on the differentiation rate of the explants. The effect of different plant growth regulators （6-BA, NAA） concentration ratios on the proliferation of adventitious buds was explored. The results showed that the optimal concentrations of tribenuron-methy1 and thifensulfuron-methy1 in SU herbicides were both 0.25 mg/L, while the optimal concentration of bensulfuron was 0.50 mg/L;the most suitable concentrations for IMI herbicides such imazaquin and imazapyr were both 1.50 mg/L;the highest proliferation rate of adventitious buds was observed in MS+1.0 mg/L 6-BA+0.5 mg/L NAA medium, which was 287.0%;the optimal concentration of herbicides determined based on watermelon inbred line D66 materials was also suitable for screening cotyledon explants of watermelon hybrids （Wunong 8, Black Beauty, Tianwang 1, Zaojia 8424）.

Key words: cotyledon explants, ALS inhibitory herbicides, watermelon inbred line, watermelon hybrid, adventitious bud proliferation, SU herbicides, IMI herbicides