水禽细小病毒选择压力与基因重组分析

doi:10.14088/j.cnki.issn0439-8114.2023.11.033

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (11): 191-197.doi: 10.14088/j.cnki.issn0439-8114.2023.11.033

水禽细小病毒选择压力与基因重组分析

汪宏才, 商雨, 郭云清, 曾哲, 张蓉蓉, 温国元, 罗青平

湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室/动物胚胎工程及分子育种湖北省重点实验室,武汉 430064

收稿日期:2022-10-17 出版日期:2023-11-25 发布日期:2023-12-25
通讯作者: 罗青平（1971-）,女,湖北武汉人,研究员,博士,主要从事预防兽医学研究,（电话）027-87284950（电子信箱）Qingping0523@163.com。
作者简介:汪宏才（1971-）,男,安徽桐城人,副研究员,主要从事预防兽医学研究,（电话）027-87284950（电子信箱）whcemail@163.com。
基金资助:
国家现代农业产业技术体系专项资金项目（CARS-41-G13）; 湖北省农业科技创新中心项目（2019-620-000-001-017）

Selection pressure and gene recombination analysis of waterfowl parvovirus

WANG Hong-cai, SHANG Yu, GUO Yun-qing, ZENG Zhe, ZHANG Rong-rong, WEN Guo-yuan, LUO Qing-ping

Institute of Animal Husbandry and Veterinary Sciences/Key Laboratory of Animal Bacterial Disease Prevention and Control Formulations of the Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Hubei Academy of Agricultural Sciences,Wuhan 430064,China

Received:2022-10-17 Online:2023-11-25 Published:2023-12-25

摘要/Abstract

摘要： 通过对临床水禽细小病毒进行PCR检测、基因组测序和进化分析,确定分离毒株的基因型。进一步通过Paml、RDP软件对分离株的基因组进行选择压力和基因重组分析,确定分离株的演化趋势。结果表明,分离的3株水禽细小病毒均属于新型鹅细小病毒,分别命名为YiCH株、AnQ株和XiY株,基因组长度分别为5 056、5 056、5 068 bp。3个分离株与新型鹅细小病毒M15株亲源关系最近,其中XiY株两端ITR区域191~196位、4 878~4 883位均有6个碱基插入。对分离株进行选择压力分析,分离株VP蛋白检测出3个正选择位点,分别为116Q、261A、485S。重组分析显示,分离株YiCH株和AnQ株都存在重组现象,以XiY株为主要亲本株、GPV 06-0329株为次要亲本株重组而成。

关键词: 水禽细小病毒, 新型鹅细小病毒, 分离鉴定, 选择压力, 基因重组

Abstract: By conducting PCR detection, genome sequencing, and evolutionary analysis on clinical waterfowl parvovirus, the genotype of the isolated strain was determined.Further selection pressure and gene recombination analysis were conducted on the genome of the isolated strains using Paml and RDP software to determine the evolutionary trend of the isolated strains. The results showed that the three strains of waterfowl parvovirus isolated belonged to the novel goose parvovirus, named YiCH strain, AnQ strain, and XiY strain, with genome lengths of 5 056, 5 056, and 5 068 bp, respectively. The three isolates had the closest phylogenetic relationship with the novel goose parvovirus M15 strain, with 6 base insertions at positions 191~196 and 4 878~4 883 in the ITR region at both ends of the XiY strain. Selection pressure analysis was conducted on the isolated strains, and three positive selection sites were detected for the VP protein of the isolated strains, namely 116Q, 261A, and 485S. Recombination analysis showed that both the YiCH and AnQ strains exhibited recombination phenomenon, with XiY strain as the main parent strain and GPV 06-0329 strain as the secondary parent strain.

Key words: waterfowl parvovirus, novel goose parvovirus, isolation and identification, select pressure, gene recombination

中图分类号:

S855.3

汪宏才, 商雨, 郭云清, 曾哲, 张蓉蓉, 温国元, 罗青平. 水禽细小病毒选择压力与基因重组分析[J]. 湖北农业科学, 2023, 62(11): 191-197.

WANG Hong-cai, SHANG Yu, GUO Yun-qing, ZENG Zhe, ZHANG Rong-rong, WEN Guo-yuan, LUO Qing-ping. Selection pressure and gene recombination analysis of waterfowl parvovirus[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(11): 191-197.

参考文献

[1] SOLIMAN M A, ERFAN A M, SAMY M, et al.Detection of novel goose parvovirus disease associated with short beak and dwarfism syndrome in commercial ducks[J]. Animals, 2020,10(10): 1833.
[2] 方定一. “小鹅瘟”的介绍[J]. 中国畜牧兽医, 1962(8): 19-20.
[3] 傅宏庆, 姚志兰, 王永娟, 等. 鸭短喙型小鹅瘟病毒SBDS-GPV JS01株的分离鉴定及其对雏鸭致病性的研究[J]. 中国兽医科学, 2020,50(10): 1286-1293.
[4] ISIDAN H, TURAN T, ATASOY M O, et al.Molecular analysis of goose parvovirus field strains from a Derzsy’s disease outbreak reveals local European-associated variants[J]. Archives of virology, 2021,166(7): 1931-1942.
[5] WAN C, LIU R, CHEN C, et al.Novel goose parvovirus in domestic Linwu sheldrakes with short beak and dwarfism syndrome, China[J]. Transboundary and emerging diseases, 2019,66(5): 1834-1839.
[6] PALYA V, ZOLNAI A, BENYEDA Z, et al.Short beak and dwarfism syndrome of mule duck is caused by a distinct lineage of goose parvovirus[J]. Avian pathology, 2009,38(2): 175-180.
[7] LI D L, ZHANG L D, CHEN S H, et al.Detection and molecular characterization of two genotypes of goose parvoviruses isolated from growing period geese and cherry valley ducks in China[J]. Avian diseases, 2019,63(3): 411-419.
[8] YU K X, MA X L, SHENG Z Z, et al.Identification of goose-origin parvovirus as a cause of newly emerging beak atrophy and dwarfism syndrome in ducklings[J]. Journal of clinical microbiology, 2016,54(8): 1999-2007.
[9] ZHAO G W, HUANG T T, WU D, et al.Diagnosis and characterization of duck beak atrophy and dwarfism syndrome in Chongqing of China[J]. Veterinary research forum, 2019,10(2): 169-172.
[10] CHEN S L, WANG S, CHENG X X, et al.Isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in China[J]. Archives of virology, 2016,161(9): 2407-2416.
[11] BIAN G Z, MA H B, LUO M P, et al.Identification and genomic analysis of two novel duck-origin GPV-related parvovirus in China[J]. BMC veterinary research, 2019,15(1): 1833-1839.
[12] XIAO S F, CHEN S L, CHENG X X, et al.The newly emerging duck-origin goose parvovirus in China exhibits a wide range of pathogenicity to main domesticated waterfowl[J]. Veterinary microbiology, 2017,203: 252-256.
[13] LI C F,LI Q,CHEN Z Y,et al.Novel duck parvovirus identified in cherry valley ducks (anas platyrhynchos domesticus), China[J]. Infection, genetics and evolution, 2016,44: 278-280.
[14] 贾婧宇,秘清灵,李勇霖,等. 3株樱桃谷鸭源新型鹅细小病毒的分离及分子特征解析[J]. 中国动物传染病学报,2022,30(2):7-13.
[15] 汪宏才, 温国元, 张腾飞, 等. 新城疫病毒阳性选择区域氨基酸与毒力相关性研究[J]. 中国家禽, 2018,40(21): 26-30.
[16] MARTIN D P, MURRELL B, GOLDEN M, et al. RDP4: Detection and analysis of recombination patterns in virus genomes[J]. Virus evolution, 2015,1(1): vev003.
[17] 傅秋玲,程龙飞, 万春和, 等. 半番鸭胚中鹅细小病毒的分离鉴定及其基因组分子特征分析[J]. 畜牧兽医学报,2017,48(11): 2148-2156.
[18] MARTIN D P, VARSANI A, ROUMAGNAC P, et al.RDP5:A computer program for analyzing recombination in, and removing signals of recombination from, nucleotide sequence datasets[J]. Virus evolution, 2021,7(1): 1-3.
[19] 程由铨, 胡奇林, 陈少莺, 等. 番鸭细小病毒和鹅细小病毒生化及基因组特性的比较[J]. 中国兽医学报, 2001(05): 429-433.
[20] FAN W, SUN Z, SHEN T, et al.Analysis of evolutionary processes of species jump in waterfowl parvovirus[J]. Frontiers in microbiology, 2017,8: 421.
[21] WANG J, LING J, WANG Z, et al.Molecular characterization of a novel Muscovy duck parvovirus isolate: Evidence of recombination between classical MDPV and goose parvovirus strains[J]. BMC veterinary research, 2017,13(1): 1238-1246.
[22] LI P, LIN S, ZHANG R, et al.Isolation and characterization of novel goose parvovirus-related virus reveal the evolution of waterfowl parvovirus[J]. Transboundary and emerging diseases, 2018,65(2): 284-295.
[23] WANG S, CHENG X X, CHEN S L, et al.Identification of a novel goose parvovirus (GPV) recombinant associated with short beak and dwarfism syndrome in Mainland China, 2015[J]. Infection, genetics and evolution, 2016,41: 289-291.
[24] FU Q L, HUANG Y, WAN C H, et al.Genomic and pathogenic analysis of a Muscovy duck parvovirus strain causing short beak and dwarfism syndrome without tongue protrusion[J]. Research in veterinary science, 2017,115: 393-400.
[25] WANG S, CHENG X X, CHEN S Y, et al.Genetic characterization of a potentially novel goose parvovirus circulating in Muscovy duck flocks in Fujian Province, China[J]. The Journal of veterinary medical science, 2013,75(8): 1127-1130.
[26] ZHU Y, ZHOU Z, HUANG Y, et al.Identification of a recombinant Muscovy duck parvovirus (MDPV) in Shanghai, China[J]. Veterinary microbiology, 2014,174(3-4): 560-564.
[27] SHEN H, ZHANG W, WANG H, et al.Identification of recombination between Muscovy duck parvovirus and goose parvovirus structural protein genes[J]. Archives of virology, 2015,160(10): 2617-2621.
[28] SHIEN J H, WANG Y S, CHEN C H, et al.Identification of sequence changes in live attenuated goose parvovirus vaccine strains developed in Asia and Europe[J]. Avian pathology, 2008,37(5): 499-505.
[29] 王浩, 田先举, 张烁, 等. 2株鹅细小病毒全基因组特征分析[J]. 畜牧兽医学报, 2013,44(4): 602-609.
[30] 黄冠雄. 2015-2016年广东省小鹅瘟病毒分子流行病学调查[D]. 广州:仲恺农业工程学院, 2017.

水禽细小病毒选择压力与基因重组分析

Selection pressure and gene recombination analysis of waterfowl parvovirus

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