蚕豆荚甾醇的提取及其动力学研究和热力学分析

doi:10.14088/j.cnki.issn0439-8114.2023.11.023

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (11): 131-136.doi: 10.14088/j.cnki.issn0439-8114.2023.11.023

蚕豆荚甾醇的提取及其动力学研究和热力学分析

杨旭¹, 诸爱士², 廖瑜晨²

1.浙江微松冷链科技有限公司,杭州 311121;
2.浙江科技学院生物与化学工程学院,杭州 310023

收稿日期:2022-07-04 出版日期:2023-11-25 发布日期:2023-12-25
通讯作者: 诸爱士（1966-）,男,浙江湖州人,副教授,主要从事传质、农产品加工研究,（电话）13757155300（电子信箱）zhuaishi86@163.com。
作者简介:杨旭（1990-）,女,河北石家庄人,工程师,硕士,主要从事传热与传质研究,（电话）13738053823（电子信箱）1185167918@qq.com。

The extraction process of the sterols from faba pods and its kinetic investigation and thermodynamic analysis

YANG Xu¹, ZHU Ai-shi², LIAO Yu-chen²

1. Zhejiang Wason Cold Chain Technology Co., Ltd., Hangzhou 311121, China;
2. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China

Received:2022-07-04 Online:2023-11-25 Published:2023-12-25

摘要/Abstract

摘要： 为了提高副产物老蚕豆荚的利用率,以乙醇水溶液为提取剂,研究了蚕豆荚甾醇的提取工艺,并对其提取过程进行动力学研究和热力学分析。通过单因素试验考察确定较优的液料比、乙醇体积分数及搅拌速度;于不同提取温度下测得溶液中的甾醇浓度与提取时间的关系,选用一级动力学平板模型,对所得动力学试验数据进行拟合;计算蚕豆荚甾醇提取过程的活化能、内扩散系数、吉布斯自由能、焓和熵。结果表明,从蚕豆荚中成功提取了甾醇,甾醇收率可达6.2 mg/g;液料比、乙醇体积分数、搅拌速度、提取温度、提取时间等对甾醇收率均有影响;适宜的提取工艺为液料比20∶1、乙醇体积分数80%、搅拌速度200 r/min、提取时间1.0 h;一级动力学平板模型适用于描述甾醇提取过程;在考察温度范围内,提取过程的活化能为22.50 kJ/mol;内扩散系数值随着提取温度的升高从2.08×10^-10 m²/min增大到4.95×10^-10 m²/min;各提取温度下提取过程的吉布斯自由能都小于0,显示从老蚕豆荚中提取甾醇的过程是一个自发过程;蚕豆荚甾醇提取过程的焓为28.26 kJ/mol以及熵为102.26 J/（mol·K）,表明蚕豆荚甾醇提取过程是一个吸热、熵增的过程。研究所得结果可为蚕豆荚甾醇提取的规模化应用提供参考。

关键词: 蚕豆荚, 甾醇, 提取, 模型, 动力学, 热力学

Abstract: In order to improve the utilization rate of the byproduct old faba pods, the extraction technology of the sterols from faba pods was studied with ethanol aqueous solution as the extraction agent, and the kinetic investigation and thermodynamic analysis of the extraction process were carried out. The optimal liquid-solid ratio, ethanol volume fraction and stirring rate were determined by single factor test investigation; the relationship between sterols concentration and extraction time was measured at different extraction temperatures, the first-order kinetic plate model was used to fit the experimental data; the activation energy, internal diffusion coefficient, Gibbs free energy, enthalpy and entropy of the extraction process of sterols from faba pods were calculated. The results showed that the sterols were successfully extracted from faba pods, and the yield of sterols was 6.2 mg/g. The liquid-solid ratio, ethanol volume fraction, stirring rate, extraction temperature and extraction time had effects on sterols yield. The suitable extraction process was the liquid-solid ratio of 20∶1, ethanol volume fraction of 80%, stirring rate of 200 r/min, and extraction time of 1.0 h. The first-order kinetic plate model was suitable for describing the extraction process of sterols. The activation energy of extraction was 22.50 kJ/mol in the range of temperatures examined. The internal diffusion coefficient increased from 2.08×10^-10 m²/min to 4.95×10^-10 m²/min with increasing extraction temperature. The Gibbs free energy of the extraction process was less than zero at all extraction temperatures, indicating that the extraction of sterol from old faba pods was a spontaneous process. The enthalpy and entropy of the extraction process of sterols from faba pods were 28.26 kJ/mol and 102.26 J/（mol·K）, which indicated that the extraction process of sterols from faba pods was endothermic and increased entropy. The results could provide reference for large-scale application of the extraction of faba pods sterols.

Key words: faba pod, sterols, exctraction, model, kinetics, thermodynamics

中图分类号:

TQ645.98

杨旭, 诸爱士, 廖瑜晨. 蚕豆荚甾醇的提取及其动力学研究和热力学分析[J]. 湖北农业科学, 2023, 62(11): 131-136.

YANG Xu, ZHU Ai-shi, LIAO Yu-chen. The extraction process of the sterols from faba pods and its kinetic investigation and thermodynamic analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(11): 131-136.

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蚕豆荚甾醇的提取及其动力学研究和热力学分析

The extraction process of the sterols from faba pods and its kinetic investigation and thermodynamic analysis

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