简易扁板海绵萜酚类成分的生物活性研究

doi:10.14088/j.cnki.issn0439-8114.2023.10.027

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (10): 154-157.doi: 10.14088/j.cnki.issn0439-8114.2023.10.027

简易扁板海绵萜酚类成分的生物活性研究

刘馨联, 庄江超, 周心行, 汤纪航, 张胜元, 王洁

浙江海洋大学食品与药学学院,浙江舟山 316022

收稿日期:2022-03-15 发布日期:2023-11-14
通讯作者: 王洁（1990-）,女,山东德州人,讲师,博士,主要从事海洋天然活性成分的研究,（电话）13701836056（电子信箱）011103@zjou.edu.cn。
作者简介:刘馨联（1996-）,女,山东蓬莱人,在读硕士研究生,研究方向为海洋天然活性成分,（电话）18858393534（电子信箱）lxl19960817@163.com。
基金资助:
浙江省省属高校基本科研业务费资助项目（2019J00012）

Research on the biological activities of terpene phenolic compounds from Plakortis simplex

LIU Xin-lian, ZHUANG Jiang-chao, ZHOU Xin-hang, TANG Ji-hang, ZHANG Sheng-yuan, WANG Jie

School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, Zhejiang,China

Received:2022-03-15 Published:2023-11-14

摘要/Abstract

摘要： 对采自中国南海西沙群岛的简易扁板海绵（Plakortis simplex）进行化学成分研究,运用LC-MS法结合HPLC法高效追踪分离和富集其萜酚类化合物,并采用MTT法和荧光素酶双报告基因检测法分别检测萜酚类化合物的抗肿瘤活性和抗炎活性。结果表明,从该海绵的正丁醇萃取物中共富集和分离了8个已知的萜酚类化合物,分别为Plakordiol A（1）、Plakordiol B（2）、Plakordiol C（3）、Plakordiol D（4）、（7R,10R）-Hydroxycurcudiol（5）、（7R,10S）-Hydroxycurcudiol（6）、（7R*,10R*）-Abolene（7）和（7R*,10S*）-Abolene（8）,为4对差向异构体。在20 mmol/L浓度下,8个萜酚类化合物均对人乳腺癌细胞MCF-7表现出一定的细胞毒活性,其中Plakordiol A（1）和Plakordiol C（3）对9-顺视黄酸诱导的RXRα的转录具有一定的抑制活性,与模型组相比存在显著差异（P<0.05或P<0.01）,而Plakordiol D（4）促进9-顺视黄酸激活RXRα的转录活性,与模型组相比存在显著差异（P<0.01）;Plakordiol A（1）可抑制TNF-α诱导的NF-κB的激活,且与模型组相比存在显著差异（P<0.05）。

关键词: 简易扁板海绵（Plakortis simplex）, 萜酚类化合物, 抗肿瘤活性, 抗炎活性

Abstract: The chemical composition of Plakortis simplex collected from Xisha Islands in the South China Sea was studied. The terpene phenolic compounds from Plakortis simplex were tracked and enriched efficiently by LC-MS combined with HPLC. The antitumor and anti-inflammatory activities of terpene phenolic compounds were detected by MTT assay and luciferase double reporter gene assay respectively. The results showed that eight known terpene phenolic compounds, Plakordiol A（1）, Plakordiol B（2）, Plakordiol C（3）, Plakordiol D（4）, （7R,10R）-Hydroxycurcudiol（5）, （7R,10S）-Hydroxycurcudiol（6）, （7R*,10R*）-Abolene（7） and （7R*,10S*）-Abolene（8）, which were four pairs of differential isomers, were enriched and isolated from the n-butanol extract of the sponge. All the compounds showed cytotoxicity against human breast cancer MCF-7 cells at the concentration of 20 mmol/L. Plakordiol A（1） and Plakordiol C（3） showed inhibitory activity against the transcription of RXRα induced by 9-cis-retinoic acid, with significant differences compared to the model group （P<0.05 or P<0.01）. While Plakordiol D（4） promoted the transcriptional activity of RXRα, with significant differences compared with the model group （P<0.01）. Plakordiol A（1） inhibited the activation of NF-κB induced by TNF-α and had significant differences compared to the model group （P<0.05）.

Key words: Plakortis simplex, terpene phenolic compounds, antitumor activity, anti-inflammatory activity

中图分类号:

R284

刘馨联, 庄江超, 周心行, 汤纪航, 张胜元, 王洁. 简易扁板海绵萜酚类成分的生物活性研究[J]. 湖北农业科学, 2023, 62(10): 154-157.

LIU Xin-lian, ZHUANG Jiang-chao, ZHOU Xin-hang, TANG Ji-hang, ZHANG Sheng-yuan, WANG Jie. Research on the biological activities of terpene phenolic compounds from Plakortis simplex[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(10): 154-157.

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简易扁板海绵萜酚类成分的生物活性研究

Research on the biological activities of terpene phenolic compounds from Plakortis simplex

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