草鱼摄食前后促食欲因子和抑制食欲因子的变化规律

doi:10.14088/j.cnki.issn0439-8114.2023.10.025

摘要/Abstract

摘要： 通过荧光定量PCR检测了草鱼（Ctenopharyngodon idella）摄食前后脑和肠道组织中胃饥饿素（Ghrelin）、神经肽Y（Neuropeptide Y,NPY）、可卡因-苯丙胺调节转录肽（Cocaine amphetamine regulated transcript,CART）、胆囊收缩素（Cholecystokinin,CCK）和瘦素（Leptin）的基因表达,探究食欲因子的变化规律。结果表明,摄食前2 h,草鱼脑组织中ghrelin mRNA表达水平达到最大峰值（P<0.05）,随后显著下降,在投喂后的4 h内,其表达水平没有显著性变化（P>0.05）。脑中促食欲因子npy mRNA在摄食前2 h内的表达量显著高于摄食后2 h（P<0.05）,而在肠道中表达量存在相反的变化趋势。脑和前肠组织中抑制食欲因子cck、cart和leptin mRNA在摄食前2 h表达丰度较低,而在摄食后1~2 h内,其含量显著上升（P<0.05）。摄食后草鱼血浆中GH含量表现出先上升后下降的趋势,并在摄食后1 h达到高峰。

关键词: 草鱼（Ctenopharyngodon idella）, 促食欲因子, 抑制食欲因子, GH

Abstract: The transcriptional levels of ghrelin, npy, cart, cck and leptin in the brain and intestinal tissues of grass carp before and after feeding were detected by real-time fluorescence quantitative PCR to explore the variation laws of their mRNA transcripts. The results showed that transcription levels of ghrelin mRNA in the brain of grass carp reached the maximum peak 2 h before feeding （P<0.05）, and then decreased significantly, but there were no significant changes in the expression levels within 4 h after feeding （P>0.05）. The mRNA expression level of npy in the brain was significantly higher within 2 h before feeding than 2 h after feeding（P<0.05）, while its mRNA expression presented an opposite trend in the foregut. Meanwhile, the expression levels of anorexigenic factors （cck, cart and leptin） in brain and foregut kept stable at a low level 2 h before feeding, while a significant increase was observed 1~2 h after feeding in the fed group （P<0.05）. The GH content in plasma after feeding exhibited a trend of rising first and then decreasing, the peak value was delayed to 1 h after feeding.

Key words: Ctenopharyngodon idella, orexigenic factors, anorexigenic factors, GH

中图分类号:

S917.4

郭薇, 杨慧君, 莫爱杰, 孙俊霄, 翟昱翔, 矣林园, 袁勇超. 草鱼摄食前后促食欲因子和抑制食欲因子的变化规律[J]. 湖北农业科学, 2023, 62(10): 143-148.

GUO Wei, YANG Hui-jun, MO Ai-jie, SUN Jun-xiao, ZHAI Yu-xiang, YI Lin-yuan, YUAN Yong-chao. Pre- and postprandial changes of orexigenic factors and anorexigenic factors in grass carp （Ctenopharyngodon idella）[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(10): 143-148.

参考文献

[1] VOLKOFF H, XU M, MACDONALD E, et al.Aspects of the hormonal regulation of appetite in fish with emphasis on goldfish, Atlantic cod and winter flounder: Notes on actions and responses to nutritional, environmental and reproductive changes[J]. Comparative biochemistry and physiology, part A. 2009, 153:8-12.
[2] VASILEIOU I,PATSOURAS D, PATSOURIS E, et al.Ghrelin and toxicity: Recent findings and future Challenges[J]. J Appl Toxicol, 2013, 33:238-245.
[3] 田娟,何艮,麦康森,等.鱼类食欲调控研究进展[J].动物营养学报,2016,28(4):984-998.
[4] VOLKOFF H.The neuroendocrine regulation of food intake in fish: A review of current knowledge[J]. Frontiers in neuroscience, 2016, 10: 540.
[5] RONNESTAD I, GOMES A S, MURASHITA K, et al.Appetite-controlling endocrine systems in teleosts[J]. Frontiers in endocrinology,2017, 8: 73.
[6] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.中国渔业统计年鉴2021[M].北京:中国农业出版社,2021.
[8] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408.
[9] FLIK G, HUISING M, GORISSEN M.Peptides and proteins regulating food intake: A comparative view[J]. Animal biology, 2006, 56(4): 447-473.
[10] LI L,FANG J,LIANG X F,et al.Effect of feeding stimulants on growth performance, feed intake and appetite regulation of mandarin fish,Siniperca chuatsi[J].Aquaculture research,2019,50(12):3684-3691.
[11] NADERMANN N, VOLKOFF H.Effects of short-term exercise on food intake and the expression of appetite-regulating factors in goldfish[J]. Peptides, 2020, 123: 170182.
[12] JØRGENSEN E H, BERNIER N J, MAULE A G, et al. Effect of long-term fasting and a subsequent meal on mRNA abundances of hypothalamic appetite regulators, central and peripheral leptin expression and plasma leptin levels in rainbow trout[J]. Peptides, 2016, 86: 162-170.
[13] FENG K E,ZHANG G U I R,WEI K A I J,et al. Molecular cloning, tissue distribution, and ontogenetic expression of ghrelin and regulation of expression by fasting and refeeding in the grass carp (Ctenopharyngodon idellus)[J]. Journal of experimental zoology part A:Ecological genetics and physiology,2013,319(4):202-212.
[14] LIANG X, HAN J, XUE M, et al.Growth and feed intake regulation responses to anorexia, adaptation and fasting in Japanese seabss, Lateolabrax japonicas when fishmeal is totally replaced by plant protein[J]. Aquaculture, 2019, 498: 528-538.
[15] VALEN R, JORDAL A E O, MURASHITA K, et al. Postprandial effects on appetite-related neuropeptide expression in the brain of Atlantic salmon, Salmo salar[J]. General and comparative endocrinology, 2011, 171(3): 359-366.
[16] FRØILAND E, MURASHITA K, JORGENSEN E H, et al. Leptin and ghrelin in anadromous Arctic charr: Cloning and change in expressions during a seasonal feeding cycle, Gen[J]. Comp Endocrinol, 2010, 165: 136-143.
[17] BAGNASCO M, KALRA P S, KALRA S P.Ghrelin and leptin pulse discharge in fed and fasted rats[J]. Endocrinology, 2002, 143(2): 726-729.
[18] UNNIAPPAN L F, CANOSA, PETER R E. Orexigenic actions of ghrelin in goldfish: Feeding-induced changes in brain and gut mRNA expression and serum levels, and responses to central and peripheral injections[J]. Neuroendocrinology,2004,79:100-108.
[19] MURASHITA K,FUKADA H, HOSOKAWA H, et al.Changes in cholecystokinin and peptide Y gene expression with feeding in yellowtail (Seriola quinqueradiata): Relation to pancreatic exocrine regulation[J]. Comp Biochem Physiol B, 2007, 146: 318-325.
[20] 曹磊,梁宏伟,李忠,等.黄颡鱼神经肽Y基因 (NPY) cDNA全序列的克隆及其表达特征分析[J].西北农林科技大学学报(自然科学版),2013,41(7):1-7,14.
[21] NARNAWARE Y K, PETER R E.Effects of food deprivation and refeeding on neuropeptide Y (NPY) mRNA levels in goldfish[J]. Comp Biochem Physiol B, 2001, 129: 633-637.
[22] CUPPLES W A.Regulation of body weight, Am J Physiol Regul Integr[J]. Physiol, 2002, 282: 1264-1266.
[23] MACDONALD E,VOLKOFF H. Neuropeptide Y (NPY), Cocaine and amphetamine regulated transcript (CART) and cholecystokinin (CCK) in winter skate (Raja ocellata): cDNA cloning, tissue distribution and mRNA expression responses to fasting[J]. Gen Comp Endocrinol, 2009, 161: 252-261.
[24] PETERSON B C, WALDBIESER G C, RILEYJR L G, et al.Pre- and postprandial changes in orexigenic and anorexigenic factors in channel catfish (Ictalurus punctatus)[J]. General and comparative endocrinology, 2012, 176(2):231-239.
[25] RUBIO V C, SÁNCHEZ-VÁZQUEZ F J, MADRID J A. Role of cholecystokinin and its antagonist proglumide on macronutrient selection in European sea bass Dicentrarchus labrax, L[J]. Physiology & behavior, 2008, 93(4-5): 862-869.
[26] LE H T M D,ANGOTZI A R, EBBESSON L O E, et al. The ontogeny and brain distribution dynamics of the appetite regulators NPY,CART and pOX in larval Atlantic cod (Gadus morhua L.)[J]. PLoS one, 2016, 11(4): e0153743.
[27] MACDONALD E, VOLKOFF H.Cloning, distribution and effects of season and nutritional status on the expression of neuropeptide Y(NPY), cocaine and amphetamine regulated transcript (CART) and cholecystokinin(CCK) in winter flounder (Pseudopleuronectes americanus)[J]. Hormones and behavior, 2009, 56(1): 58-65.
[28] DE PEDRO N, BJÖRNSSON. Regulation of food intake by neuropeptides and hormones[A]. in: D Houlihan, T. Boujard, M. Jobling (Eds), Food Intake in Fish[M]. Oxford UK: Blackwell science, 2001, 269-296.
[29] GORISSEN M H A G, FLIK G, HUISING M O. Peptides and proteins regulating food intake: A comparative view[J]. Biol, 2006, 56: 447-473.
[30] KLING P, RØNNESTAD I, STEFANSSON S O, et al. A homologous salmonid leptin radioimmunoassay indicates elevated plasma leptin levels during fasting of rainbow trout[J]. General and comparative endocrinology, 2009, 162(3): 307-312.
[31] WON E T, BALTZEGAR D A, PICHA M E, et al.Cloning and characterization of leptin in a perciform fish, the striped bass (Morone saxatilis): Control of feeding and regulation by nutritional state[J]. General and comparative endocrinology, 2012, 178(1): 98-107.
[32] KIM J H, LEGGATT R A, CHAN M, et al.Effects of chronic growth hormone overexpression on appetite-regulating brain gene expression in coho salmon[J]. Molecular and cellular endocrinology, 2015, 413: 178-188.
[33] JOHNSSON J I, BJÖRNSSON B T. Growth hormone increases growth rate, appetite and dominance in juvenile rainbow trout, Oncorhynchus mykiss[J]. Animal behaviour,1994, 48(1): 177-186.
[34] ABRAHAMS M V, SUTTERLIN A.The foraging and antipredator behaviour of growth-enhanced transgenic Atlantic salmon[J]. Animal behaviour, 1999, 58(5): 933-942.
[35] CANOSA L F,GÓMEZ-REQUENI P, CERDÁ-REVERTER J M. Integrative cross-talk between food intake and growth function[M]. Trout: From Physiology to Conservation Nova Publishers, 2013: 179-239.

[1]	彭宏鑫, 周正, 陈亚飞, 蒋懿康, 蒋慧. 六个甜高粱品种在阿拉尔市的产量及品质比较[J]. 湖北农业科学, 2023, 62(1): 12-17.
[2]	陈光明, 杨艳娟, 梁杏梅, 余朝良. 云南鼠尾草不同部位扦插繁殖研究[J]. 湖北农业科学, 2022, 61(9): 88-91.
[3]	秦惠珍, 农月香, 邹蓉, 柴胜丰, 韦霄, 史艳财, 韦记青. 东兴金花茶枝、花、果实在树冠上的分布格局及其相互关系[J]. 湖北农业科学, 2022, 61(18): 103-107.
[4]	肖飞. 金黄疏肝应激散对靶动物草鱼的安全性试验[J]. 湖北农业科学, 2022, 61(18): 151-154.
[5]	刘英枭, 袁小红. 刺梨果渣中蔷薇酸的提取工艺研究[J]. 湖北农业科学, 2021, 60(5): 103-107.
[6]	杨威, 廖华杰, 胡程棚, 张波, 曾荣俊, 刘晓瑞, 何兴恒. 拉萨裸裂尻鱼胚胎发育观察[J]. 湖北农业科学, 2021, 60(2): 116-118.
[7]	程琳, 陈晓明, 谭文婧, 蓝肖, 陈琴, 陈代喜, 戴俊, 黄开勇, 梁机, 贾盛强, 莫绍锋, 黄咏昕. 杉木二代种子园种实产量与质量分析[J]. 湖北农业科学, 2021, 60(18): 97-100.
[8]	韩永亮, 崔江慧, 罗河月, 方路斌, 常金华. 品种和种植密度对高粱主要农艺性状和产量的影响[J]. 湖北农业科学, 2021, 60(17): 19-22.
[9]	赵乙桦, 钟江斌, 李威霖, 祁得胜, 孙士浩, 邹登朗, 都玉蓉, 马建滨. 宽叶独行菜提取物对大鼠胃肠动力及SP和Ghrelin的影响[J]. 湖北农业科学, 2020, 59(9): 130-132.
[10]	施满容, 罗义发. 不同栽培模式对金线莲产量和品质的影响[J]. 湖北农业科学, 2020, 59(5): 96-100.
[11]	巩朋成, 谭颖, 詹云峰, 程慧芬, 李灵, 邓张惠, 王自强. 一种高精度的无人驾驶农机系统研究[J]. 湖北农业科学, 2020, 59(4): 141-146.
[12]	王宏博, 李维红, 杨晓玲, 高雅琴, 席斌. 青贮甜高粱替代青贮玉米对羔羊生产性能和养分消化代谢的影响[J]. 湖北农业科学, 2020, 59(16): 112-114.
[13]	蒋凡, 买凯乐. 杉木组培苗生根质量优化研究[J]. 湖北农业科学, 2020, 59(11): 81-83.
[14]	李楠, 施力军, 陈彩姻, 高丽霞. 蔓性千斤拔最佳采收期及根部总黄酮含量提取工艺研究[J]. 湖北农业科学, 2019, 58(20): 143-146.
[15]	杜雪树, 夏明元, 李进波, 戚华雄. 利用鄂早18 Ghd7基因型改良扬稻6号生育期效应的研究[J]. 湖北农业科学, 2018, 57(23): 27-31.