关键词: 高效液相色谱-串联质谱法, 维生素B₁, 维生素B₆, 烟酸, 烟酰胺, 咖啡因, 保健食品

Abstract: A high-performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of vitamin B₁, vitamin B₆, nicotinic acid, niacinamide, and caffeine in health foods was established. Hawthorn yeast tablets, B vitamins tablets, multi B vitamins tablets, comprehensive nutrition tablets （for pregnant women）, melatonin tablets, multi vitamin mineral chewing tablets （for children）, Mount Huangshan Ganoderma lucidum spore powder, propolis soft capsules, and health oral liquid were used as test materials. Nine kinds of health food were dissolved in deionized water, and the filtrate was passed through the chromatographic column CAPCELL PAK C18 （150 mm×2.1 mm, 5 μm） to separate. Mobile phase was 0.1% formic acid solution -0.1% formic acid methanol solution. Gradient elution and positive ion mode of electric spray ion source were adopted. The detection mode was multiple reaction monitoring （MRM）, spray voltage was 5 000 V, the ion source temperature was 500 ℃, and the target compounds in 9 health foods were separated and determined. The results showed that the target compounds of vitamin B₁, vitamin B₆, Nicotinic acid, niacinamide, and caffeine had a good linear relationship at the concentration ranging from 5 to 500 μg/L, with R² all greater than 0.990 0, recovery rates ranging from 75.4% to 94.5%, and relative standard deviation （RSD） ranging from 1.1% to 15.6%. The quantitative limits for the five target compounds of vitamin B₁, vitamin B₆, Nicotinic acid, niacinamide, and caffeine were 2.10, 4.47, 3.93, 5.46, and 1.53 μg/kg, respectively. Compared with existing national standards, this method had high sensitivity, simple and fast pre-treatment operation, and strong anti-interference ability, and could simultaneously determine the content of five target compounds in different dosage forms of health food.

Key words: high performance liquid chromatography tandem mass spectrometry, vitamin B₁, vitamin B₆, nicotinic acid, nicotinamide, caffeine, health foods