独脚金内酯对甘蓝型油菜种子的引发作用及其机制研究

doi:10.14088/j.cnki.issn0439-8114.2023.09.002

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (9): 6-13.doi: 10.14088/j.cnki.issn0439-8114.2023.09.002

独脚金内酯对甘蓝型油菜种子的引发作用及其机制研究

祝四¹, 邓凤玲¹, 赵光¹, 朝洪波², 李春生¹, 顾建伟¹

1.湖北工程学院生命科学技术学院,湖北孝感 432000;
2.郑州大学农业科技学院,郑州 450001

收稿日期:2022-05-12 出版日期:2023-09-25 发布日期:2023-10-24
通讯作者: 顾建伟,讲师,主要从事油菜重要性状的遗传解析及分子育种研究,（电子信箱）gu03jia@mail.hzau.edu.cn。
作者简介:祝四（1995-）,女,湖北黄冈人,在读硕士研究生,研究方向为油菜种子活力调控机理,（电话）18671279628（电子信箱）1425655741@qq.com;共同第一作者,邓凤玲（1996-）,女,湖北宜昌人,硕士,主要从事油菜种质资源开发和利用研究,（电话）15871337839（电子信箱）gang07042020@163.com。
基金资助:
湖北省教育厅重大项目（15ZD034）; 中央引导地方科技发展专项（2018ZYYD035）; 高校服务乡村振兴科技支撑行动计划项目（BXLBX0725）; 湖北省教育厅中青年人才项目（Q20182707）

Study on the effect and mechanism of strigolactone on seed priming in Brassica napus L.

ZHU Si¹, DENG Feng-ling¹, ZHAO Guang¹, CHAO Hong-bo², LI Chun-sheng¹, GU Jian-wei¹

1. College of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, Hubei, China;
2. School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China

Received:2022-05-12 Online:2023-09-25 Published:2023-10-24

摘要/Abstract

摘要： 为了解甘蓝型油菜（Brassica napus L.）种子活力的生物学过程并开发一种新型种子引发剂,分析了不同浓度的独脚金内酯（GR24）对甘蓝型油菜种子萌发的引发效应。结果表明,10.00 μmol/L的GR24能显著促进种子萌发和幼苗生长并提高萌发种子和幼苗中抗氧化酶活性和抗胁迫物质的积累。基因表达分析表明,10.00 μmol/L的GR24处理可能通过调节ABA和GA合成及信号通路相关基因的表达来缓解ABA对种子萌发和幼苗生长的抑制作用,提高GA对种子萌发和幼苗生长的促进作用。

关键词: 甘蓝型油菜（Brassica napus L.）, 独脚金内酯, 萌发, 种子活力, 种子引发

Abstract: To understand the biological process of Brassica napus L. seed vigor and develop an initiator for seed viability, the priming effects of the strigolactone （GR24） on seed germination in Brassica napus L. under different concentrations were analyzed. The results showed that 10.00 μmol/L GR24 significantly promoted seed germination and seedling growth, and increased the activities of antioxidant enzymes and the accumulation of anti-stress substances in the germinated seeds and seedlings. Gene expression analysis demonstrated that treatment with 10.00 μmol/L GR24 could effectively alleviate the inhibitory impact of ABA and enhance the promoting influence of GA on seed germination and seedling growth by regulating the expression of genes involved in ABA and GA synthesis and signaling pathways.

Key words: Brassica napus L., strigolactone, germination, seed vigor, seed priming

中图分类号:

祝四, 邓凤玲, 赵光, 朝洪波, 李春生, 顾建伟. 独脚金内酯对甘蓝型油菜种子的引发作用及其机制研究[J]. 湖北农业科学, 2023, 62(9): 6-13.

ZHU Si, DENG Feng-ling, ZHAO Guang, CHAO Hong-bo, LI Chun-sheng, GU Jian-wei. Study on the effect and mechanism of strigolactone on seed priming in Brassica napus L.[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(9): 6-13.

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独脚金内酯对甘蓝型油菜种子的引发作用及其机制研究

Study on the effect and mechanism of strigolactone on seed priming in Brassica napus L.

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