关键词: 高尿酸血症, 网络药理学, 分子对接技术, 核桃（Juglans regia L.）, 鞣花酸, 作用机制

Abstract: Based on network pharmacology and molecular docking, the mechanism of action of Juglans regia L. in the treatment of Hyperuricemia （HUA） was analyzed, and the Traditional Chinese medicine systems pharmacology database and analysis platform （TCMSP） was used to predict the target of active components in walnut. OMIM, GeneCards and DrugBank databases were used to search for HUA disease targets. Cytoscape 3.7.2 software was used for target-active ingredient-disease network mapping, and Metascape platform was used for enrichment analysis. Molecular docking was performed using AutoDockTools 1.5.6 software to verify the binding degree of active ingredients and important targets. The results showed that 5 active compounds of walnut were selected, and 16 common targets of walnut and HUA were identified. The highly enriched signaling pathways include cancer signaling pathway, chemical carcinogenic-receptor activation signaling pathway, advanced glycation endproducts receptor for advanced glycation end products signaling pathway in diabetic complications, and Kaposi sarcoma-associated herpes virus signaling pathway. The molecular docking test showed that the main active compounds in the walnut were closely bound to important targets.

Key words: hyperuricemia, network pharmacology, molecular docking technology, walnut（Juglans regia L.）, ellagic acid, mechanism