四种茶花品种花朵的香气挥发物分析

doi:10.14088/j.cnki.issn0439-8114.2023.07.024

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (7): 137-142.doi: 10.14088/j.cnki.issn0439-8114.2023.07.024

四种茶花品种花朵的香气挥发物分析

杨敏^1,2, 谢焰锋¹, 许林¹, 向琮琳^1,2, 傅劭¹, 陈宝林¹, 魏鸣¹, 陈晓桂^1,2, 李清源¹

1.武汉市农业科学院林业果树研究所,武汉 430075;
2.华中农业大学园艺林学学院,武汉 430070

收稿日期:2021-12-05 出版日期:2023-07-25 发布日期:2023-08-15
通讯作者: 李清源（1982-）,男,湖北武汉人,工程师,博士,主要从事茶花育种研究,（电话）13114375216（电子信箱）gavin1982@163.com。
作者简介:杨敏（1997-）,女,云南昆明人,在读硕士研究生,研究方向为林木遗传育种,（电话）15287146587（电子信箱）2683330189@qq.com。
基金资助:
武汉市农业科学院创新项目（QNCX202311）

Analysis of aroma volatiles in flowers of four camellia varieties

YANG Min^1,2, XIE Yan-feng¹, XU Lin¹, XIANG Cong-lin^1,2, FU Shao¹, CHEN Bao-lin¹, WEI Ming¹, CHEN Xiao-gui^1,2, LI Qing-yuan¹

1. Institute of Forestry and Pomology, Wuhan Academy of Agricultural Sciences, Wuhan 430075, China;
2. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China

Received:2021-12-05 Online:2023-07-25 Published:2023-08-15

摘要/Abstract

摘要： 以C37（Camellia japonica C37）、甜凯特（Camellia japonica Sweet Emily Kate）、姬（Camellia lutchuensis Hime）、烈香（Camellia japonica Liexiang）4种香花型茶花品种为材料,采用顶空固相微萃取-气相色谱联用（SPME/GC-MS）技术,分析4种茶花品种在盛花期花朵的香气挥发物成分及相对含量。结果表明,在C37、甜凯特、姬、烈香中检测出香气挥发物,分别为27、10、19、27种,主要包括醇类、酯类、醛类、芳香族化合物、酚类、醚类、萜类、酮类、烷烃、脂肪酸10类化合物。正己醇、苯甲醛、苯甲醇、苯乙醛、苯甲酸甲酯、苯乙醇、萘、水杨酸甲酯为4种茶花品种主要的挥发物成分,这些化合物分别占C37、甜凯特、姬、烈香总挥发物含量的86.84%、90.32%、93.81%、82.20%,苯环类化合物为主要花香成分。苯甲酸甲酯在C37和姬中的相对含量最高,分别为39.37%和42.00%;而苯乙醇（31.60%）在烈香中的相对含量最高;正己醇（70.22%）为甜凯特的主要挥发物。4种茶花品种花朵中酯类及醇类物质的相对含量远高于其他化合物,是茶花主要的挥发物。

关键词: 茶花, 花朵, 香气挥发物, 顶空固相微萃取-气相色谱联用（SPME/GC-MS）

Abstract: Four fragrant camellia varieties, namely C37 （Camellia japonica C37）, Sweet Emily Kate （Camellia japonica Sweet Emily Kate）, Hime （Camellia lutchuensis Hime）, and Liexiang （Camellia japonica Liexiang）, were used as materials to analyze the volatiles components and relative content of the flowers of these four camellia varieties during their blooming period using headspace solid-phase microextraction gas chromatography （SPME/GC-MS） technology. The results showed that aroma volatiles compounds were detected in C37, Sweet Emily Kate, Hime, and Liexiang, with 27, 10, 19, and 27 types, mainly including alcohols, esters, aldehydes, aromatic compounds, phenols, ethers, terpenoids, ketones, alkanes, and fatty acids. N-hexanol, Benzaldehyde, Benzyl alcohol, Phenylacetaldehyde, Methyl benzoate, Phenethyl alcohol, Naphthalene, and Methyl salicylate were the main volatiles components of the four camellia varieties. These compounds accounted for 86.84%, 90.32%, 93.81%, and 82.20% of the total volatiles content of C37, Sweet Emily Kate, Hime, and Liexiang, respectively. Benzene ring compounds were the main floral components. The relative content of Methyl benzoate in C37 and Hime was the highest, 39.37% and 42.00%, respectively; the relative content of Phenethyl alcohol （31.60%） in Liexiang was the highest; N-hexanol （70.22%） was the main volatiles compound of Sweet Emily Kate.The relative content of esters and alcohols in the flowers of four camellia varieties was much higher than that of other compounds, making them the main volatiles of camellia.

Key words: camellia, flowers, aroma volatiles, headspace solid-phase microextraction gas chromatography （SPME/GC-MS）

中图分类号:

S685.14

杨敏, 谢焰锋, 许林, 向琮琳, 傅劭, 陈宝林, 魏鸣, 陈晓桂, 李清源. 四种茶花品种花朵的香气挥发物分析[J]. 湖北农业科学, 2023, 62(7): 137-142.

YANG Min, XIE Yan-feng, XU Lin, XIANG Cong-lin, FU Shao, CHEN Bao-lin, WEI Ming, CHEN Xiao-gui, LI Qing-yuan. Analysis of aroma volatiles in flowers of four camellia varieties[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(7): 137-142.

参考文献

[1] PICHERSKY E,DUDAREVA N.Scent engineering:Toward the goal of controlling how flowers smell[J]. Trends in biotechnology,2007,25(3):105-110.
[2] 邹晶晶,蔡璇,曾祥玲,等.桂花不同品种开花过程中香气活性物质的变化[J].园艺学报,2017,44(8):1517-1534.
[3] 林榕燕,钟淮钦,黄敏玲,等.晚香玉花香成分时空动态变化的研究[J].核农学报,2017,31(12):2434-2442.
[4] 李晋华,晏慧君,杨锦红,等.香水月季复合群(Rosa odorata Complex)花香成分分析[J].西南农业学报,2018,31(3):587-591.
[5] 郑冉冉,吴景芝,谷志佳,等.玫瑰香味玫红百合和橙香味紫红花滇百合的花香成分研究[J].浙江大学学报(农业与生命科学版),2021,47(1):32-42.
[6] KNUDSEN J T,ERIKSSON R,GERSHENZON J,et al.Diversity and distribution of floral scent[J]. The botanical review,2006,72(1):1-120.
[7] PELLMYR O, THIEN L B.Insect reproduction and floral fragrances-keys to the evolution of the angiosperms[J].Taxon,1986(35): 76-85.
[8] WHITEHEA D,MICHAE L,PEAKAL L.Integrating floral scent, pollination ecology and population genetics[J]. Functional ecology, 2010, 23(5):863-874.
[9] HSIAO Y Y, PAN Z J, HSU C C, et al.Research on orchid biology and biotechnology[J]. Plant and cell physiology,2011,52(9):1467-1486.
[10] 樊玉花,瞿素萍,王珍珍,等.香味茶花远缘杂交亲本云南核果茶(Pyrenaria yunnanensis Hu)香气成分分析[J].江苏农业科学,2020,48(2):192-196.
[11] JULLIEN F, GAO J,OREL G,et al.Analysis of tissue-specific emission of volatiles by the flowers of six Camellia species[J]. Flavour and fragrance journal, 2010, 23(2):115-120.
[12] HATTAN J I, SHINDO K, ITO T, et al.Identification of a novel hedycaryol synthase gene isolated from Camellia brevistyla flowers and floral scent of Camellia cultivars.[J]. Planta, 2016, 243(4):959-972.
[13] 范正琪,李纪元,李辛雷,等.基于HS-SPME/GC-MS分析山茶品种‘克瑞墨大牡丹’花器官香气成分[J].植物研究,2014,34(1):136-142.
[14] 王洁,李辛雷,范正琪,等.不同茶梅品种花朵挥发物研究[J].广西植物,2018,38(7):934-942.
[15] 傅劭,陈宝林,李清源,等.茶花良种‘烈香’[J].湖北林业科技,2020,49(2):82-83.
[16] 冯楠. 蜡梅花香挥发物测定及2个萜烯合酶基因功能初步研究[D].武汉:华中农业大学,2017.
[17] 孔滢,孙明,潘会堂,等.花香代谢与调控研究进展[J].北京林业大学学报,2012,34(2):146-154.
[18] 杨吉龙,尹陈茜,谭君,等.遮光处理对两种观赏栀子开花特性及挥发物的影响[J].西北植物学报,2020,40(11):1940-1950.
[19] 韩丛聪,荀守华,姜天华,等.刺槐属6种材料鲜花芳香成分分析[J].园艺学报,2017,44(3):557-565.
[20] 钱晓慧,陈龙清,李彪,等.云南地区不同基因型蜡梅花香气成分分析[J].西南农业学报,2021,34(4):834-841.
[21] 邱建生,张念念,刘童童,等.中华蜜蜂在滇山茶上的访花行为研究[J].生物资源,2018,40(1):52-56.
[22] 范正琪,李纪元,田敏,等.三个山茶花种(品种)香气成分初探[J].园艺学报,2006(3):592-596.
[23] 甘秀海,梁志远,王道平,等.3种山茶属花香气成分的HS-SPME/GC-MS分析[J].食品科学,2013,34(6):204-207.
[24] 邱建生,张彦雄,陈菊艳,等.12个山茶属植物花的挥发性物质研究[J].林业科学研究,2015,28(3):358-364.
[25] 王洁,李辛雷,殷恒福,等.茶梅冬星不同花期及花器官挥发物[J].云南农业大学学报(自然科学),2018,33(5):904-910.
[26] 王洁,李辛雷,殷恒福,等.茶梅品种‘冬玫瑰’不同花期及花器官的香气组成成分分析[J].植物资源与环境学报,2018, 27(1):37-43.
[27] DUDAREVA N,D AURIA J C,NAM K H,et al. Acetyl-CoA:Benzylalcohol acetyltransferase-an enzyme involved in floral scent production in Clarkia breweri[J].The plant journal,1998,14(3): 297-304.

四种茶花品种花朵的香气挥发物分析

Analysis of aroma volatiles in flowers of four camellia varieties

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