黄芩苷对猪源肠外致病性大肠杆菌的体内保护作用

doi:10.14088/j.cnki.issn0439-8114.2023.05.022

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (5): 124-128.doi: 10.14088/j.cnki.issn0439-8114.2023.05.022

黄芩苷对猪源肠外致病性大肠杆菌的体内保护作用

任明星¹, 肖涌¹, 付书林¹, 宗冰冰^1,2,3, 邱银生¹

1.武汉轻工大学动物科学与营养工程学院,武汉 430023;
2.农业农村部畜禽细菌病防治制剂创制重点实验室,武汉 430064;
3.畜禽病原微生物学湖北省重点实验室,武汉 430064

收稿日期:2023-03-08 出版日期:2023-05-25 发布日期:2023-06-12
通讯作者: 宗冰冰（1990-）,女,河南安阳人,讲师,主要从事动物病原微生物致病机制及药物疫苗研发工作,（电话）15926369021（电子信箱）bbzong@foxmail.com;邱银生（1964-）,男,湖北武汉人,教授,主要从事兽医药理学和新兽药研发工作,（电话）13387505028（电子信箱）qiuyinsheng@aliyun.com。
作者简介:任明星（1997-）,女,河南洛阳人,在读硕士研究生,研究方向为动物安全生产与致病机制,（电话）17739090949（电子信箱）1427992718@qq.com。
基金资助:
农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室开放课题（KLPCAAB-2022-05）; 湖北省重大科技创新计划项目（2022BBAD055）

Protective effect of baicalin on porcine extra-intestinal pathogenic Escherichia coli in vivo

REN Ming-xing¹, XIAO Yong¹, FU Shu-lin¹, ZONG Bing-bing^1,2,3, QIU Yin-sheng¹

1. School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China;
2. Key Laboratory of Prevention and Control Agents for Animal Bacteriosis （Ministry of Agriculture and Rural Affairs）, Wuhan 430064, China;
3. Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Wuhan 430064, China

Received:2023-03-08 Online:2023-05-25 Published:2023-06-12

摘要/Abstract

摘要： 为探究黄芩苷对猪源肠外致病性大肠杆菌（Extraintestinal pathogenic Escherichia coli,ExPEC）感染小鼠后的保护作用,采用小鼠存活率试验和组织载菌量试验评估黄芩苷对小鼠的保护作用,比较感染组和黄芩苷治疗组小鼠临床表现、存活率、组织载菌量及病理变化。结果表明,猪源肠外致病性大肠杆菌感染后小鼠状态萎靡不振、食欲下降,黄芩苷治疗组小鼠状态较感染组状态好;黄芩苷治疗组小鼠存活率相对于感染组有一定提升;黄芩苷治疗组小鼠心脏、肝脏、脾脏和肾脏中组织载菌量及病理变化相对于感染组均极显著降低（P<0.01）。

关键词: 黄芩苷, 猪源肠外致病性大肠杆菌（Extraintestinal pathogenic Escherichia coli, ExPEC）, 小鼠, 存活率, 组织载菌量

Abstract: To explore the protective effect of baicalin on mice infected with porcine extraintestinal pathogenic Escherichia coli, the survival rate assay and colonization assay of mice were selected to evaluate the protective effect of baicalin. The clinical feature, survival rate, tissue bacterial load and pathological changes of mice in the infected group and baicalin treatment group were compared. The results showed that the mental state of mice treated by baicalin was better than that of the mice infected by PCN033. The survival rate of the baicalin treatment group was improved compared with that of the infection group. Bacterial counts in heart, liver, spleen and kidney of mice treated with baicalin were significantly reduced compared with those of mice infected with PCN033 （P<0.01）.

Key words: baicalin, porcine extraintestinal pathogenic Escherichia coli, mice, survival rate, colonization

中图分类号:

R378
R284

任明星, 肖涌, 付书林, 宗冰冰, 邱银生. 黄芩苷对猪源肠外致病性大肠杆菌的体内保护作用[J]. 湖北农业科学, 2023, 62(5): 124-128.

REN Ming-xing, XIAO Yong, FU Shu-lin, ZONG Bing-bing, QIU Yin-sheng. Protective effect of baicalin on porcine extra-intestinal pathogenic Escherichia coli in vivo[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(5): 124-128.

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黄芩苷对猪源肠外致病性大肠杆菌的体内保护作用

Protective effect of baicalin on porcine extra-intestinal pathogenic Escherichia coli in vivo

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