三株新型海参致病菌的分离鉴定及特性分析

doi:10.14088/j.cnki.issn0439-8114.2023.04.021

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (4): 118-122.doi: 10.14088/j.cnki.issn0439-8114.2023.04.021

三株新型海参致病菌的分离鉴定及特性分析

许潇冉¹, 葛长字², 张玉群², 刘洪展²

1.昌吉州市场监督管理局产品质量检验所,新疆昌吉 831100;
2.山东大学海洋学院近海生态动力学实验室,山东威海 264209

收稿日期:2021-11-08 出版日期:2023-04-25 发布日期:2023-05-12
通讯作者: 刘洪展（1975-）,男,讲师,博士,主要从事水产鱼类病害与免疫学研究,（电子信箱）1019281412@qq,com。
作者简介:许潇冉（1993-）,女,新疆奇台人,工程师,硕士,主要从事质量技术检验工作,（电话）13999548567（电子信箱）973417896@qq.com。
基金资助:
山东省重点研发计划项目（2019GHY112003）; 山东大学基本业务费青年培养项目（2015ZQXM2024）; 山东省自然科学基金项目（ZR2016DM18）; 国家自然科学基金项目（31972796）

Isolation, identification and characteristic analysis of three new pathogenic bacteria of Apostichopus japonicus

XU Xiao-ran¹, GE Chang-zi², ZHANG Yu-qun², LIU Hong-zhan²

1. Product Quality Inspection Institute of Changji Market Supervision and Administration Bureau, Changji 831100, Xinjiang, China;
2. Offshore Ecological Dynamics Laboratory, Marine College, Shandong University, Weihai 264209, Shandong, China

Received:2021-11-08 Online:2023-04-25 Published:2023-05-12

摘要/Abstract

摘要： 从感染严重腐皮病的仿刺参（Apostichopus japonicus）皮肤溃烂组织中分离得到3株优势致病菌WY-1、WY-8、LY-2,通过形态学观察、生化鉴定、16S rRNA分子鉴定、药物敏感性分析、生长特性分析等方法对分离菌株进行鉴定。WY-1、WY-8、LY-2在2216E固体培养基上排列呈葡萄状、乳白色,革兰氏染色呈阳性。人工回接感染试验表明,3株菌对仿刺参有较强的致病力,可以引起皮肤溃疡等症状,且与自然感染症状一样。通过16S rRNA分子测序并构建系统发育树证实,菌株WY-8、WY-1、LY-2分别与溶血葡萄球菌（Staphylococcus haemolyticus）、腐生葡萄球菌（Staphylococcus saprophytius）和表皮葡萄球菌（Staphylococcus epidermidis）同源性最高,并在系统发育树中各自聚为一支。结合形态、生化鉴定,WY-8、WY-1、LY-2分别为溶血葡萄球菌、腐生葡萄球菌和表皮葡萄球菌。药敏试验表明,海洋水产中常用的抗生素四环素和土霉素对3株病原葡萄球菌均有抑制作用。生长特性试验表明,3株病原葡萄球菌具有适应高温、低盐的生长特征。

关键词: 仿刺参（Apostichopus japonicus）, 葡萄球菌（Staphylococcus）, 腐皮病, 16S rRNA, 生长特性

Abstract: Three dominant pathogenic bacteria WY-1, WY-8 and LY-2 were isolated from the skin canker tissue of Apostichopus japonicus infected with severe skin rot. The isolated strains were identified by morphological observation, biochemical identification, 16S rRNA molecular identification, drug sensitivity analysis, growth characteristics analysis and other methods. WY-1, WY-8 and LY-2 were arranged in a grape-like shape on 2216E solid medium with the color of milky white, and Gram staining was positive. The artificial infection test showed that the three strains of bacteria had strong pathogenicity to pseudoacanthus japonicus, and could cause the skin ulcer and other symptoms, which were the same as natural infection symptoms. Through 16S rRNA molecular sequencing and phylogenetic tree construction, it was confirmed that the strains WY-8, WY-1 and LY-2 had the highest homology with Staphylococcus haematolyticus, Staphylococcus saprophytius and Staphylococcus epidermidis, respectively, and clustered into one branch in the phylogenetic tree. Combined with morphological and biochemical identification, WY-8, WY-1 and LY-2 were Staphylococcus haematolyticus,Staphylococcus saprophytius and Staphylococcus epidermidis, respectively. The drug sensitivity test showed that tetracycline and oxytetracycline, commonly used antibiotics in marine aquatic products, had inhibitory effects on three strains of pathogenic Staphylococcus. The growth characteristics test showed that the three strains of pathogenic Staphylococcus had the growth characteristics of adapting to high temperature and low salt.

Key words: Apostichopus japonicus, Staphylococcus, skin ulceration syndrome, 16S rRNA, growth characteristics

中图分类号:

S947.9

许潇冉, 葛长字, 张玉群, 刘洪展. 三株新型海参致病菌的分离鉴定及特性分析[J]. 湖北农业科学, 2023, 62(4): 118-122.

XU Xiao-ran, GE Chang-zi, ZHANG Yu-qun, LIU Hong-zhan. Isolation, identification and characteristic analysis of three new pathogenic bacteria of Apostichopus japonicus[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(4): 118-122.

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三株新型海参致病菌的分离鉴定及特性分析

Isolation, identification and characteristic analysis of three new pathogenic bacteria of Apostichopus japonicus

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