藻菌生物膜处理含镍废水及其胞外聚合物变化研究

doi:10.14088/j.cnki.issn0439-8114.2023.03.022

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (3): 135-140.doi: 10.14088/j.cnki.issn0439-8114.2023.03.022

藻菌生物膜处理含镍废水及其胞外聚合物变化研究

任芸芸¹, 张莎², 裴挫萍¹, 张志林¹

1.铜川职业技术学院, 陕西铜川 727031;
2.陕西科技大学,西安 710021

收稿日期:2021-12-08 发布日期:2023-04-20
作者简介:任芸芸（1985-）,女,河北枣强人,讲师,硕士,主要从事重金属废水的微生物植物联合修复的研究,（电话）13700292590（电子信箱）393390644@qq.com。
基金资助:
陕西省职业技术教育学会科研规划课题（SZJYB19-288）; 铜川市有突出贡献拔尖人才专项资金项目

Study on treatment of nickel-containing wastewater by alga-bacteria biofilm and changes of the extracellular polymeric substances

REN Yun-yun¹, ZHANG Sha², PEI Cuo-ping¹, ZHANG Zhi-lin¹

1. Tongchuan Vocational and Technical College, Tongchuan 727031,Shaanxi,China;
2. Shaanxi University of Science and Technology,Xi'an 710021,China

Received:2021-12-08 Published:2023-04-20

摘要/Abstract

摘要： 为探究藻菌生物膜处理含镍废水的可行性,对自然城市水体的藻菌富集挂膜得到藻菌生物膜,并在不同运行参数下对含镍溶液进行处理,研究镍的吸附量及藻菌生物膜胞外聚合物（EPS）含量在不同条件下的变化。结果表明,经富集挂膜的藻类属于绿藻门小球藻属,其对含镍废水有较高的处理效果,在pH为8、温度为35 ℃时处理效果最佳;且能在3 d内达到镍吸附平衡,去除率达70%以上,同时,藻菌生物膜多聚糖含量随时间的变化与镍吸附量呈一定的正相关,证明其为镍吸附中的主要作用物质,而ATP含量则更能反映藻菌生物膜受胁迫的过程。藻菌生物膜在镍的胁迫下,胞外聚合物含量随胁迫程度升高而减少;从吸附量来看,其对镍的耐受值为10 mg/L,在此范围内,吸附量随镍浓度的升高而升高,最高达2.358 mg,继续升高镍浓度则吸附量明显下降。

关键词: 藻菌生物膜, 胞外聚合物（EPS）, 镍（Ni）, 吸附量, 去除率

Abstract: To explore the feasibility of algal-bacteria biofilm's treatment for nickel-containing wastewater, alga-bacteria biofilm was obtained by filming algae and bacteria enrichment in natural urban water, and nickel-containing solution was treated under different operating parameters to study the adsorption amount of nickel and the change of extracellular polymer substance（EPS） content of algal bacteria biofilm under different conditions. The results showed that the algas after enrichment and filming belonged to Chlorella, which had a higher treatment effect on nickel-containing wastewater. The treatment effect was best when pH was 8 and the temperature was 35 ℃. Nickel adsorption equilibrium was reached within 3 days and the removal rate was more than 70%. At the same time, the content of polysaccharides in alga-bacteria biofilm with time was positively correlated with nickel adsorption amount, which proved that it was the main substance in nickel adsorption, while ATP content could reflect the stress process of alga-bacteria biofilm. Under the stress of nickel, the content of extracellular polymer substances in alga-bacteria biofilm decreased with the increase of stress. From the perspective of adsorption amount, its tolerance to nickel was 10 mg/L. Within this range, adsorption amount increased with the increase of the nickel concentration, up to 2.358 mg. However, adsorption amount decreased significantly when the nickel concentration continued to increase.

Key words: algae biofilm, extracellular polymer substance （EPS）, nickel （Ni）, adsorption amount, removal rate

中图分类号:

X703

任芸芸, 张莎, 裴挫萍, 张志林. 藻菌生物膜处理含镍废水及其胞外聚合物变化研究[J]. 湖北农业科学, 2023, 62(3): 135-140.

REN Yun-yun, ZHANG Sha, PEI Cuo-ping, ZHANG Zhi-lin. Study on treatment of nickel-containing wastewater by alga-bacteria biofilm and changes of the extracellular polymeric substances[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(3): 135-140.

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藻菌生物膜处理含镍废水及其胞外聚合物变化研究

Study on treatment of nickel-containing wastewater by alga-bacteria biofilm and changes of the extracellular polymeric substances

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