湖北农业科学 ›› 2022, Vol. 61 ›› Issue (24): 140-143.doi: 10.14088/j.cnki.issn0439-8114.2022.24.030

• 检测分析 • 上一篇    下一篇

湿法消解可见光度法测定莴苣中铁元素的含量

刘博文, 王欢, 吴俣, 熊波, 刘晓霞   

  1. 武汉东湖学院护理与健康管理学院,武汉 430212
  • 收稿日期:2021-11-02 出版日期:2022-12-25 发布日期:2023-01-18
  • 通讯作者: 刘晓霞,女,教授,主要从事应用化学分析研究,(电话) 027-81933509(电子信箱)417249002@qq.com。
  • 作者简介:刘博文(2000-),男,湖北黄石人,专业为应用化学与分析检测,(电话) 027-81933509 (电子信箱) 1073718116@qq.com。
  • 基金资助:
    2021年度湖北省教育厅科研计划项目(B2021291); 武汉东湖学院2021年“东湖未来之星”大学生科研基金项目

Determination of iron in lettuce by wet digestion visible spectrophotometry

LIU Bo-wen, WANG Huan, WU Yu, XIONG Bo, LIU Xiao-xia   

  1. School of Nursing and Health Management, Wuhan Donghu University, Wuhan 430212,China
  • Received:2021-11-02 Online:2022-12-25 Published:2023-01-18

摘要: 针对邻菲罗啉分光光度法测定莴苣(Lactuca sativa Linn.)铁含量试验条件进行优化,研究了最佳吸收波长的选择、盐酸羟胺使用量、乙酸-醋酸钠使用量以及邻菲罗啉使用量和显色时间对铁标准溶液吸光度的影响。结果表明,该方法测得莴苣粉末中铁含量为8.54 μg/g,相对标准偏差为0.26%,试验的重复性和准确度均较好。

关键词: 分光光度法, 莴苣(Lactuca sativa Linn.), 铁元素

Abstract: The experimental conditions were optimized for the determination of iron content in Lactuca sativa Linn. by o-phenanthroline spectrophotometry. Some factors affecting the absorbance of iron standard solution were studied. These factors included the selection of the best absorption wavelength, the dosage of hydroxylamine hydrochloride, acetic acid sodium acetate and o-phenanthroline, and color development time. The results showed that the content of iron in Lactuca sativa Linn. powder was 8.54 μg/g, the RSD was 0.26%, and the repeatability and accuracy were good.

Key words: spectrophotometry, lettuce(Lactuca sativa Linn.), iron

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