鸡肝脏中能量代谢相关膜蛋白基因及其上游转录因子的筛选与鉴定

doi:10.14088/j.cnki.issn0439-8114.2022.24.028

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (24): 132-135.doi: 10.14088/j.cnki.issn0439-8114.2022.24.028

鸡肝脏中能量代谢相关膜蛋白基因及其上游转录因子的筛选与鉴定

申杰¹, 李刚², 黄涛¹, 皮劲松¹, 龚炎长², 潘爱銮¹, 吴艳¹, 张昊¹, 付明¹, 梁振华¹

1.湖北省农业科学院畜牧兽医研究所/湖北省农业科技创新中心/动物胚胎与分子育种湖北省重点实验室,武汉 430064;
2.湖北农垦对外经济交流中心,武汉 430064;
3.华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉 430064

收稿日期:2022-09-29 出版日期:2022-12-25 发布日期:2023-01-18
作者简介:申杰（1965-）,男,河南淅川人,副研究员,主要从事家禽遗传育种、健康养殖技术研究与推广,（电话）15927657060（电子信箱）15927657060@163.com。
基金资助:
国家现代农业产业技术体系资助项目（CARS-41）; 动物胚胎与分子育种湖北省重点实验室开放课题（KLAEMB-2020-01）; 湖北省农业科技创新中心项目（2016-620-000-023）; 湖北省技术创新重大项目（2018ABA112）

Identification and screening of energy metabolism-related membrane protein genes and their upstream transcription factors in chicken liver

SHENG Jie¹, LI Gang², HUANG Tao¹, PI Jin-song¹, GONG Yan-zhang³, PAN Ai-luan¹, WU Yan¹, ZHANG Hao¹, FU Ming¹, LIANG Zhen-hua¹

1. Institute of Animal Husbandry and Veterinary/Hubei Academy of Agricultural Sciences/Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Lab of Animal Embryo Technology and Molecular Breeding, Wuhan 430064, China;
2. Hubei Agricultural Reclamation Foreign Economic Exchange Center, Wuhan 430064,China;
3. Huazhong Agricultural University/Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Wuhan 430064, China

Received:2022-09-29 Online:2022-12-25 Published:2023-01-18

摘要/Abstract

摘要： 跨脂质分子层膜蛋白（Tran membrane protein,TMEMs）参与细胞的生理生化过程,对细胞功能的维持具有重要作用。为弄清TMEMs基因在肝脏组织中的表达规律,以不同饲养状态下蛋仔鸡和肉仔鸡的肝脏组织样本,检测8个TMEMs基因的表达情况。结果表明,TMEMs基因在肉鸡肝脏组织中维持较高表达水平,其中TMEM86A基因在肉鸡肝脏中高表达,而在蛋鸡肝脏中低表达,限饲处理会促进TMEM86A基因上调表达;通过对TMEM86A基因启动子区域转录因子预测及启动子活性检测,发现SP1、CREB1可能是TMEM86A的上游转录因子。

关键词: TMEM86A基因, 膜蛋白, 肝脏, 鸡

Abstract: Tran membrane proteins（TMEMs） participates in the physiological and biochemical processes of cells and plays an important role in the maintenance of cell function. In order to explore the expression pattern of TMEMs genes in liver tissues, the eight TMEMs genes were chosen to detect their expression levels in liver tissues of broilers and laying hens under the different feeding conditions. The results showed that TMEMs genes maintained a high expression level in the liver tissue of broilers. The expression of TMEM86A gene was high in the liver of broilers and low in the liver of laying hens, and feeding restriction would promote the up-regulated expression of TMEM86A gene. Through the prediction of transcription factors using the promoter region of TMEM86A gene and detection of the promoter activity, it was found that SP1 and CREB1 may be the upstream transcription factors of TMEM86A.

Key words: TMEM86A gene, TMEMs, liver, chicken

中图分类号:

S831

申杰, 李刚, 黄涛, 皮劲松, 龚炎长, 潘爱銮, 吴艳, 张昊, 付明, 梁振华. 鸡肝脏中能量代谢相关膜蛋白基因及其上游转录因子的筛选与鉴定[J]. 湖北农业科学, 2022, 61(24): 132-135.

SHENG Jie, LI Gang, HUANG Tao, PI Jin-song, GONG Yan-zhang, PAN Ai-luan, WU Yan, ZHANG Hao, FU Ming, LIANG Zhen-hua. Identification and screening of energy metabolism-related membrane protein genes and their upstream transcription factors in chicken liver[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 132-135.

参考文献

[1] XIE J,TANG L, LU L, et al.Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (gallus gallus)[J]. PloS one,2014, 9(7):e102204.
[2] JIANG Y,DENBOW C,MEIRI N,et al.Epigenetic-imprinting changes caused by neonatal fasting stress protect from future fasting stress[J]. Journal of neuroendocrinology, 2016,28(1):12333.
[3] MCCORMICK C C, GARLICH J D, EDENS F W.Fasting and diet affect the tolerance of young chickens exposed to acute heat stress[J]. The journal of nutrition, 1979, 109:1797-1809.
[4] TANG X, MENG Q, GAO J, et al.Label-free quantitative analysis of changes in broiler liver proteins under heat stress using swath-ms technology[J]. Scientific reports, 2015, 5: 15119.
[5] SCHMIT K, MICHIELS C.Tmem proteins in cancer: A review[J]. Frontiers in pharmacology, 2018, 9:1345.
[6] WRZESINSKI T, SZELAG M, CIESLIKOWSKI W A, et al.Expression of pre-selected tmems with predicted er localization as potential classifiers of ccrcc tumors[J]. BMC cancer, 2015, 15(1):1-18.
[7] ZHOU X, POPESCU N C, KLEIN G, et al.The interferon-alpha responsive gene tmem7 suppresses cell proliferation and is downregulated in human hepatocellular carcinoma[J]. Cancer genetics and cytogenetics, 2007, 177:6-15.
[8] DOOLAN P,CLYNES M, KENNEDY S, et al.Tmem25, reps2 and meis 1: Favourable prognostic and predictive biomarkers for breast cancer[J]. Tumor biology, 2009, 30(4): 200-209.
[9] CHENG Z, GUO J, CHEN L, et al.Overexpression of tmem158 contributes to ovarian carcinogenesis[J]. Journal of experimental & clinical cancer research, 2015, 34(1):1-9.
[10] ZHANG Q, CHEN X, ZHANG X, et al.Knockdown of tmem14a expression by rnai inhibits the proliferation and invasion of human ovarian cancer cells[J]. Bioscience reports, 2016, 36:e00298.
[11] LI H, WANG T, XU C, et al.Transcriptome profile of liver at different physiological stages reveals potential mode for lipid metabolism in laying hens[J]. BMC genomics, 2015,16(1):1-13.
[12] MORGAN R O, FISCHER P W, STEPHENS J K, et al.Thyroid hormone enhancement of drug-induced porphyrin biosynthesis in chick embryo liver cells maintained in serum-free waymouth medium[J]. Biochemical pharmacology,1976, 25:2609-2612.
[13] TIAN W, ZHENG H, YANG L, et al.Dynamic expression profile, regulatory mechanism and correlation with egg-laying performance of ACSF gene family in chicken (Gallus gallus)[J]. Scientific reports, 2018, 8(1):1-10.
[14] CHO Y K,YOON Y C,IM H, et al.Adipocyte lysoplasmalogenase TMEM86A regulates plasmalogen homeostasis and protein kinase A-dependent energy metabolism[J]. Nat Commun, 2022,13(1):1-17.
[15] IIJIMA K, ZHAO L, SHENTON C, et al.Regulation of energy stores and feeding by neuronal and peripheral creb activity in drosophila[J]. PloS one, 2009, 4:e8498.
[16] GUO F, ZHANG Y, SU L, et al.Breed-dependent transcriptional regulation of phosphoenolpyruvate carboxylase, cytosolic form, expression in the liver of broiler chickens[J]. Poultry science, 2013, 92:2737-2744.
[17] MILLER C N, MORTON H P, COONEY P T, et al.Acute exposure to high-fat diets increases hepatic expression of genes related to cell repair and remodeling in female rats[J]. Nutrition research, 2014, 34:85-93.
[18] WANG H, LI K, MEI Y, et al.Sp1 suppresses mir-3178 to promote the metastasis invasion cascade via upregulation of triobp[J]. Molecular therapy:Nucleic acids, 2018, 12:1-11.

鸡肝脏中能量代谢相关膜蛋白基因及其上游转录因子的筛选与鉴定

Identification and screening of energy metabolism-related membrane protein genes and their upstream transcription factors in chicken liver

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