掌叶覆盆子种内与种间遗传多样性的RAPD分析

doi:10.14088/j.cnki.issn0439-8114.2022.21.034

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (21): 183-188.doi: 10.14088/j.cnki.issn0439-8114.2022.21.034

掌叶覆盆子种内与种间遗传多样性的RAPD分析

陈亦权¹, 陈珍¹, 江景勇², 刘浩正¹, 唐雨杰¹

1.台州学院生命科学学院/浙江省植物进化生态学与保护重点实验室,浙江台州 318000;
2.台州市农业科学研究院园艺所,浙江临海 317000

收稿日期:2021-10-06 出版日期:2022-11-10 发布日期:2022-12-10
通讯作者: 陈珍,女,副教授,主要从事药用与园艺植物生物技术方面的教学与研究,（电子信箱）chenzh@tzc.edu.cn。
作者简介:陈亦权（2000-）,男,浙江温州人,在读本科生,专业方向为药用植物与利用,（电话）13336924818（电子信箱）994902138@qq.com。
基金资助:
浙江省基础公益研究计划项目（LGN22C020001）; 台州市科技计划项目（21nya14）

RAPD analysis of intraspecial and interspecific genetic diversity in Rubus chingii Hu

CHEN Yi-quan¹, CHEN Zhen¹, JIANG Jing-yong², LIU Hao-zheng¹, TANG Yu-jie¹

1. College of Life Sciences, Taizhou University / Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou 318000,Zhejiang,China;
2. Institute of Horticulture, Taizhou Academy of Agricultural Sciences, Linhai 317000,Zhejiang,China

Received:2021-10-06 Online:2022-11-10 Published:2022-12-10

摘要/Abstract

摘要： 掌叶覆盆子（Rubus chingii Hu）,蔷薇科悬钩子属,未成熟果实入药,成熟果实营养丰富,是优质的“药食同源”特色植物,经济效益显著。为突破育种瓶颈,尽快提升覆盆子品质,急需开发分子标记辅助育种。以前期筛选的17个掌叶覆盆子优质株系和7个其他悬钩子属植物[山莓（Rubus corchorifolius）、三花悬钩子（Rubus trianthus）、茅莓（Rubus parvifolius）、高粱泡（Rubus lambertianus）和光滑悬钩子（Rubus tsangii）、黑莓‘赫尔'（Rubus fructicosus var. Hull）和重瓣空心泡（Rubus rosifolius var. coronarius）]为材料,筛选适合掌叶覆盆子种间和种内鉴别的RAPD引物。结果表明,25条RAPD引物扩增条带清晰,该方法重复性好,能明显区分掌叶覆盆子和其他悬钩子属植物;其中6条可区分掌叶覆盆子不同株系。

关键词: 掌叶覆盆子（Rubus chingii Hu）, 悬钩子属, 分子标记, RAPD

Abstract: Rubus chingii Hu, belongs to Rubus, Rosaceae. Its unripe fruit is used as medicine material, and its ripe fruit is rich in nutritions. Rubus chingii is a unique dual-function food with remarkable nutraceutical, pharmaceutical and economic values. It is urgent to develop molecular marker-assisted breeding and then comprehensively improve the quality of Rubus chingii fruit. Seventeen accessions of screened Rubus chingii seedlings with good traits and seven other Rubus plants, including Rubus corchorifolius,Rubus trianthus,Rubus parvifolius,Rubus lambertianus, Rubus tsangii, as well as Rubus fructicosus var. Hull and Rubus rosifolius var. coronarius, were used as materials, to select the optimal RAPD primers for intraspecial and interspecific genetic diversity analysis for Rubus chingii. The results showed 25 primers with products of clear bands and good repeatability could be used to identify the Rubus chingii seedlings and other Rubus plants. Furthermore, six primers could be applied for distinguishing different genotypes of Rubus chingii.

Key words: Rubus chingii Hu, Rubus, molecular marker, random amplified polymorphic DNA

中图分类号:

陈亦权, 陈珍, 江景勇, 刘浩正, 唐雨杰. 掌叶覆盆子种内与种间遗传多样性的RAPD分析[J]. 湖北农业科学, 2022, 61(21): 183-188.

CHEN Yi-quan, CHEN Zhen, JIANG Jing-yong, LIU Hao-zheng, TANG Yu-jie. RAPD analysis of intraspecial and interspecific genetic diversity in Rubus chingii Hu[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(21): 183-188.

参考文献

[1] 尹永飞,景志贤,张珂,等. 华东覆盆子生态适宜性区划研究[J]. 中国现代中药, 2019, 21(10): 1342-1347.
[2] WANG L J, LEI T, HAN G M, et al.The chromosome-scale reference genome of Rubus chingii Hu provides insight into the biosynthetic pathway of hydrolysable tannins[J]. The plant journal,2021, 107(5):1466-1477.
[3] 严建立, 周历萍, 张乐, 等. 掌叶覆盆子红熟果营养品质及耐贮性测定[J]. 浙江农业科学, 2019, 60(2): 242-244.
[4] CHEN Z, JIANG J Y, LI X B, et al.Bioactive compounds and fruit quality of Chinese raspberry, Rubus chingii Hu varied with genotype and phenological phase[J]. Scientia horticulturae,2021, 281: 109951.
[5] LI X B, SUN J, CHEN Z, et al.Metabolite profile and genes/proteins expression in β-citraturin biosynthesis during fruit ripening in Chinese raspberry (Rubus chingii Hu)[J]. Plant physiology and biochemistry, 2021, 163: 76-86.
[6] 国家药典委员会. 中华人民共和国药典[M].北京: 中国医药科技出版社, 2015. 382.
[7] YU G H, LUO Z Q, WANG W B, et al.Rubus chingii Hu: A review of the phytochemistry and pharmacology[J]. Frontiers in pharmacology, 2019, 10:799.
[8] CHEN Y, CHEN Z Q, GUO Q W, et al.Identification of ellagitannins in the unripe fruit of Rubus chingii Hu and evaluation of its potential antidiabetic activity[J]. Journal of agricultural and food chemistry, 2019, 67: 7025-7039.
[9] SHENG J Y, WANG S Q, LIU K H, et al.Rubus chingii Hu: an overview of botany, traditional uses, phytochemistry, and pharmacology[J]. Chinese journal of natural medicines, 2020, 18(6): 401-416.
[10] KE H H, BAO T, CHEN W.New function of polysaccharide from Rubus chingii Hu: Protective effect against ethyl carbamate induced cytotoxicity[J]. Journal of the science of food and agriculture, 2021, 101(8):3156-3164.
[11] WANG Y, CHEN Q, CHEN T, et al.Phylogenetic insights into Chinese Rubus (Rosaceae) from multiple chloroplast and nuclear DNAs[J]. Frontiers in plant science, 2016, 7:968.
[12] GRAHAM J, MC NICOL R J. Identification of red raspberry cultivars and an assessment of their relatedness using fingerprints produced by random primers[J].The journal of horticultural science, 1994, 69(1):123-130.
[13] GRAHAM J,MC NICOL R J. An examination of the ability of RAPD markers to determine the relationships within and between Rubus species[J]. Theoretical and applied genetics, 1995, 90(7-8): 1128-1132.
[14] REBECCA A, RANDELL, DIANELLA G, et al.Genetic analysis of natural hybrids between endemic and alien Rubus(Rosaceae) species in Hawai'i[J]. Conservation genetics,2004(5):217-230.
[15] 贾静波, 李维林, 吴文龙, 等. 悬钩子属植物的RAPD分析[J]. 植物资源与环境学报, 2008, 17(3):18-22.
[16] PATAMSYTÉ J, ŽVINGILA D, LABOKAS J, et al.Study of genetic diversity in wild raspberry (Rubus idaeus L.) germplasm collection using morphological characters and RAPD markers[J]. Biologia, 2008, 54: 66-74.
[17] 姚鑫, 诸炜荣, 黄宏亮, 等. 掌叶覆盆子药用有效成分变异与优良种质筛选[J]. 中国中药杂志, 2021, 46: 575-581.
[18] 段娟. 西南地区17种(33份)不同来源的野生悬钩子属植物种间遗传多样性的RAPD分析[D]. 四川雅安: 四川农业大学, 2007.
[19] 李媛媛. 树莓种质资源遗传多样性及创新研究[D]. 沈阳: 沈阳农业大学,2009.

掌叶覆盆子种内与种间遗传多样性的RAPD分析

RAPD analysis of intraspecial and interspecific genetic diversity in Rubus chingii Hu

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