湖北农业科学 ›› 2022, Vol. 61 ›› Issue (20): 216-220.doi: 10.14088/j.cnki.issn0439-8114.2022.20.040

• 生物工程 • 上一篇    下一篇

小麦主要品质相关基因的分子检测与分析

孙华卫1, 凌冬1, 张道荣1, 唐清1, 周芳菊1, 汤清益1, 刘先斌1, 王志顺1, 汤三明1, 任喜峰2   

  1. 1.襄阳市农业科学院粮食作物研究所,湖北 襄阳 441057;
    2.湖北洪山实验室,武汉 430070
  • 收稿日期:2022-08-01 出版日期:2022-10-25 发布日期:2022-11-23
  • 通讯作者: 凌 冬(1973-),男,湖北随州人,正高级农艺师,主要从事小麦育种与栽培技术研究工作,(电话)13797636755(电子信箱)125845861@qq.com。
  • 作者简介:孙华卫(1989-),男,湖北襄阳人,助理研究员,主要从事小麦遗传育种工作,(电话)15997268388(电子信箱)sunhuawei2011@163.com。
  • 基金资助:
    湖北省重点研发计划项目(2020BBA033); 湖北洪山实验室开放课题(2021hskf009)

Molecular detection and analysis of main quality related genes in wheat

SUN Hua-wei1, LING Dong1, ZHANG Dao-rong1, TANG Qing1, ZHOU Fang-ju1, TANG Qing-yi1, LIU Xian-bin1, WANG Zhi-shun1, TANG San-ming1, REN Xi-feng2   

  1. 1. Food Crop Research Institute, Xiangyang Academy of Agricultural Sciences, Xiangyang 441057, Hubei, China;
    2. Hubei Hongshan Laboratory, Wuhan 430070, China
  • Received:2022-08-01 Online:2022-10-25 Published:2022-11-23

摘要: 为了明确相关品质基因在88份亲本材料中的分布和频率,为小麦品质育种提供亲本材料,利用分子标记对Ax2*AxNBx7、By8、Dx5基因、多酚氧化酶基因以及1B/1R异位系进行检测。结果表明,高分子量谷蛋白亚基基因Ax2*AxNBx7、By8、Dx5的分布频率分别为4.55%、57.95%、92.05%、35.23%和43.18%,1B/1R异位系频率为21.59%,多酚氧化酶基因Ppo-A1a(高PPO)、Ppo-A1b(低PPO)、Ppo-D1a(低PPO)和Ppo-D1b(高PPO)频率分别为45.45%、54.55%、55.68%和44.32%。基因型Ppo-A1b/Ppo-D1a(双低PPO)的频率为32.95%,基因型Ppo-A1a/Ppo-D1a(中等PPO)和Ppo-A1b/Ppo-D1b(中等PPO)的频率分别为22.73%和21.59%。基因型Ppo-A1a/Ppo-D1b(双高PPO)的频率为22.73%。聚合多个优质基因的亲本材料较少,同时含有7+8亚基、5+10亚基、非1B/1R易位以及双位点低PPO活性(Ppo-A1b/Ppo-D1a)的材料有3份,分别为郑麦119、郑麦578和藁优5766,可作为培育优质小麦的亲本材料。

关键词: 小麦, 高分子量谷蛋白亚基, 多酚氧化酶, 1B/1R易位系

Abstract: In order to clarify the distribution and frequency of related quality genes in 88 parental materials and provide parental materials for wheat quality breeding, molecular markers were used to detect the genes related to quality including Ax2*, AxN, Bx7, By8, Dx5 genes, polyphenol oxidase genes and 1B/1R translocation. The results showed that the distribution frequencies of Ax2*, AxN, Bx7, By8 and Dx5 genes were 4.55%, 57.95%, 92.05%, 35.23% and 43.18%, respectively, and the frequency of 1B/1R translocation was 21.59%. The frequencies of polyphenol oxidase genes PPO-A1a (high PPO), PPO-A1b(low PPO), PPO-D1a (low PPO) and PPO-D1b (high PPO) were 45.45%, 54.55%, 55.68% and 44.32%, respectively. The frequency of genotype PPO-A1b/PPO-D1a (double low PPO) was 32.95%, and the frequencies of genotype PPO-A1a/PPO-D1a (medium PPO) and PPO-A1b/PPO-D1b (medium PPO) were 22.73% and 21.59%, respectively. The frequency of genotype PPO-A1a/PPO-D1b (double high PPO) was 22.73%. There were few parent materials polymering multiple high-quality genes. There were 3 materials containing 7+8,5+10 subunits, non-1B /1R translocation and double low PPO activity (ppo-A1b/ppo-D1a), which were Zhengmai 119, Zhengmai 578 and Gaoyou 5766, respectively, and could be used as parent materials for breeding high-quality wheat.

Key words: wheat, subunits of high molecular weight glutenin, polyphenol oxidase, 1B/1R translocation lines

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