湖北农业科学 ›› 2022, Vol. 61 ›› Issue (20): 136-140.doi: 10.14088/j.cnki.issn0439-8114.2022.20.026

• 贮藏·加工 • 上一篇    下一篇

酶解罗汉果提取物在口含烟加香中的应用

吴彦1, 徐赫男2, 薛云1, 刘启斌1, 刘鸿1, 李志华1, 陆漓1, 许春平3   

  1. 1.广西中烟工业有限责任公司技术中心,南宁 530001;
    2.康赞(北京)生物科技有限公司,北京 100176;
    3.郑州轻工业大学食品与生物工程学院,郑州 450002
  • 收稿日期:2021-10-21 出版日期:2022-10-25 发布日期:2022-11-23
  • 通讯作者: 徐赫男(1977-),女,辽宁沈阳人,工程师,博士,主要从事烟叶的生物提质减害及烟用香料的提取与开发研究,(电话)13609831939(电子信箱)xhnwyz@sina.com。
  • 作者简介:吴 彦(1972-),男,北京人,研究员,博士,主要从事天然烟用香原料开发及卷烟减害研究,(电话)0771-8098556(电子信箱)abelabel@126.com。
  • 基金资助:
    桂工信科技项目(20190029)

Application of Siraitia grosvenorii enzymatic hydrolysis extracts in oral tobacco flavoring

WU Yan1, XU He-nan2, XUE Yun1, LIU Qi-bin1, LIU Hong1, LI Zhi-hua1, LU Li1, XU Chun-ping3   

  1. 1. Technical Center, China Tobacco Guangxi Industrial Limited Company, Nanning 530001, China;
    2. Comzym (Beijing) Biotechnology Limited Company, Beijing 100176, China;
    3. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China
  • Received:2021-10-21 Online:2022-10-25 Published:2022-11-23

摘要: 为研究和开发具有中国特色的新型烟草制品,选取广西药食同源植物罗汉果为原料,分别采用醇提法和酶解法提取并制备罗汉果口含烟用香料。并采用同时蒸馏萃取装置对其所含挥发性成分进行萃取,采用气相色谱-质谱仪对萃取成分进行分离鉴定。结果表明,罗汉果经普通醇提法和酶解法处理后,分别检测出 22种和30种致香成分。酶解法提取的罗汉果香料的香味物质种类更为丰富,香气特性更加浓郁醇厚。将酶解法提取的罗汉果香料添加到袋装口含烟中进行加香试验,该香料添加比例为30%时口感最佳。该提取物既改善了口含烟口感协调舒适性,又保护了吸食者的身体健康,赋予产品多元化功能。

关键词: 罗汉果, GC/MS, 口含烟, 生物酶解技术, 香味成分

Abstract: In order to develop new-type tobacco products with Chinese characteristics, Siraitia grosvenorii, a medical and edible homologous plant from Guangxi, was selected as raw materials to extract and prepare the oral tobacco flavors by ethanol extraction and biological enzymatic hydrolysis technology. Flavor ingredients were extracted by simultaneous distillation extraction method. Volatile components were analyzed and identified by gas chromatography-mass spectrometry. The results showed that 22 and 30 aromatic components were separated and identified by ethanol extraction and enzymatic hydrolysis respectively. Aromatic components extracted by enzymatic hydrolysis were more abundant, and aroma characteristics were richer and mellower. The flavoring tests were carried out by adding the flavoring extract after enzymatic hydrolysis to oral tobacco products, the taste was the best when the flavoring concentration was 30%. It not only improved the coordination and comfort of the taste of oral tobacco products, but also provided consumers healthier options and gave the product diversified functions.

Key words: Siraitia grosvenorii, GC/MS, oral tobacco products, biological enzymatic hydrolysis technology, aroma components

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