铅镉胁迫下泡桐1201幼苗的生理机制响应

doi:10.14088/j.cnki.issn0439-8114.2022.20.016

摘要/Abstract

摘要： 以泡桐（Paulownia fortunei）1201为试验材料,采用水培试验的方法,研究了铅、镉胁迫影响下,其幼苗生物量、生理生化指标和亚细胞分布的一系列变化。结果表明,Pb、Cd胁迫对泡桐1201幼苗生物量的影响表现为低促高抑。泡桐1201幼苗对Cd的吸收及富集作用强于Pb,幼苗各部位重金属含量均表现为根>叶>茎,各部位生物富集系数及转运系数均呈下降趋势,泡桐1201幼苗根部起到了缓冲及保护屏障的作用,成为吸收富集Pb、Cd的主要部位。Pb、Cd胁迫下,随着胁迫浓度的升高,泡桐1201幼苗根部超氧化物歧化酶（SOD）活性先降低后升高,叶片SOD活性无显著变化;叶片过氧化氢酶（CAT）活性随胁迫浓度的增加呈先升高后降低的趋势,过氧化物酶（POD）活性与胁迫浓度呈正相关。可溶性糖含量随胁迫浓度增加呈先升高后降低的趋势,可溶性蛋白质含量先升高后降低,游离脯氨酸含量与胁迫浓度呈正相关。非蛋白巯基化合物（NPT）含量随着胁迫浓度的增加均呈先升高后降低的趋势,谷胱甘肽（GSH）含量呈降低趋势,螯合肽（PCs）含量变化较复杂,但总体上呈先升高后降低的趋势,且叶片非蛋白巯基含量显著高于根部。亚细胞分布各组分中的Cd²⁺、Pb²⁺积累量与胁迫浓度呈正相关。

关键词: 泡桐（Paulownia fortunei）1201, 生物量, 抗氧化酶, 非蛋白巯基化合物, 亚细胞分布

Abstract: A series of changes of seedling biomass, physiological and biochemical indexes and subcellular distribution of Paulownia fortunei 1201 under plumbum and cadmium stress were studied by hydroponic experiment. The results showed that the effect of plumbum and cadmium stress on seedling biomass of Paulownia fortunei 1201 was promoting at low level and suppressing at high level. Paulownia fortunei 1201 seedlings had stronger absorption and enrichment of cadmium than plumbum, and the heavy metal content in each part of the seedlings was in the order of root > leaf > stem. The bioenrichment coefficient and transport coefficient in each part of the seedlings showed a downward trend. The root of Paulownia fortunei 1201 seedlings played a role of buffer and protective barrier, becoming the main part of absorption and enrichment of plumbum and cadmium. Under plumbum and cadmium stress, super oxide dismutase（SOD） activities of Paulownia fortunei 1201 seedling roots decreased first and then increased with the increasing of stress concentration, while SOD activities of Paulownia fortunei 1201 seedling leaves had no significant change. Leaf catalase（CAT） activities increased first and then decreased with the increase of stress concentration. Peroxidase（POD） activities were positively correlated with stress concentration. Soluble sugar contents increased firstly and then decreased with the increasce of stress concentration, and the soluble protein contents increased first and then decreased, and free proline contents were positively correlated with stress concentration. With the increase of stress concentration, non-protein thiols compounds（NPT） contents increased first and then decreased, glutathione（GSH） contents decreased, phytochelatins（PCs） contents showed more complicated changes, but generally increased first and then decreased, and the contents of non-protein thiols compounds in leaves were significantly higher than those in roots. The accumulation of Cd²⁺ and Pb²⁺in subcellular distribution was positively correlated with stress concentration.

Key words: Paulownia fortunei 1201, biomass, antioxidant enzyme, non-protein thiols compounds, subcellular distribution

中图分类号:

朱秀红, 张记钟, 李哲静, 张淑静, 茹广欣. 铅镉胁迫下泡桐1201幼苗的生理机制响应[J]. 湖北农业科学, 2022, 61(20): 82-91.

ZHU Xiu-hong, ZHANG Ji-zhong, LI Zhe-jing, ZHANG Shu-jing, RU Guang-xin. The physiological response of Paulownia fortunei 1201 seedlings under plumbum and cadmium stress[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(20): 82-91.

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