湖北农业科学 ›› 2022, Vol. 61 ›› Issue (19): 110-113.doi: 10.14088/j.cnki.issn0439-8114.2022.19.021

• 畜牧·兽医 • 上一篇    下一篇

乳酸处理水体外抑菌效果及其对三黄鸡血清指标和粪便pH的影响

王树艳1, 沈前程2, 覃军1, 李小燕1, 粟永春2, 凌丁1   

  1. 1.广西农业职业技术大学动物科技学院,南宁 530007;
    2.广西金陵农牧集团有限公司,南宁 530049
  • 收稿日期:2021-04-29 出版日期:2022-10-10 发布日期:2022-11-04
  • 通讯作者: 凌 丁,教授,(电子信箱)1536101890@qq.com。
  • 作者简介:王树艳(1984-),女,黑龙江哈尔滨人,讲师,主要从事畜牧兽医教学与研究工作,(电话)13557011131(电子信箱)444086912@qq.com。
  • 基金资助:
    2017年度广西高校中青年教师基础能力提升项目(2017KY1133)

Bacteriostatic effect in vitro of lactic acid treated water and its influence on serum indexes and fecal pH of Sanhuang chicken

WANG Shu-yan1, SHEN Qian-cheng2, QIN Jun1, LI Xiao-yan1, SHU Yong-chun2, LING Ding1   

  1. 1. College of Animal Science and Technology,Guangxi Agricultural Vocational and Technical University,Nanning 530007,China;
    2. Guangxi Jinling Husbandry Group Co., Ltd., Nanning 530049,China
  • Received:2021-04-29 Online:2022-10-10 Published:2022-11-04

摘要: 为了研究乳酸处理水体外抑菌效果及其对三黄鸡血清指标和粪便pH的影响,采用平板计数法观察不同pH乳酸处理水体外抑制大肠杆菌(Escherichia coli)和沙门氏菌(Salmonella)的效果。试验分乳酸处理无菌水组、无菌水组、抗生素组和空白对照组,培养2、8、14、20、24 h后,分别对其菌落数进行计数。根据体外抑菌结果,选取1日龄三黄鸡120只,分对照组和乳酸饮水组进行饲养,测定血清总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)和γ-谷氨酰转肽酶(GGT),记录每天的粪便pH。结果表明,①乳酸处理水在pH为1~2、2~3、3~4时,大肠杆菌和沙门氏菌均在培养2 h开始平板菌落数为0;pH为4~5时,两种菌在培养8 h开始平板菌落数明显减少;pH为5~6时,大肠杆菌和沙门氏菌分别在培养24 h和14 h时菌落数明显减少。②乳酸饮水组13日龄和 25日龄三黄鸡的血清总蛋白和球蛋白显著高于对照组(P<0.05);白蛋白高于对照组(P>0.05);γ-谷草转氨酶和A/G值差异不显著(P>0.05)。与对照组比较,粪便pH差异不显著(P>0.05),但有下降趋势。由此可见,乳酸处理水pH 为1~4时能有效抑制体外大肠杆菌和沙门氏菌,三黄鸡饮用pH为3~4乳酸处理水,利于血清总蛋白、白蛋白和球蛋白生成,促进鸡群健康,增强机体免疫力,调节消化道pH。

关键词: 乳酸, 体外抑菌, 三黄鸡, 血清指标, 粪便pH

Abstract: In order to study the bacteriostatic effect of lactic acid treated water in vitro and its effect on the serum index and fecal pH of Sanhuang chicken, the plate counting method was used to observe the inhibitory effect of lactic acid with different pH on Escherichia coli and Salmonella in water. The test was divided into lactic acid treated sterile water group, sterile water group, antibiotic group and blank control group. After culturing for 2, 8, 14, 20 and 24 hours, the number of colonies was counted respectively. According to the in vitro bacteriostatic results, 120 1-day-old Sanhuang chickens were selected and fed in the control group and the lactate drinking water group. Serum total protein (TP), albumin (ALB),globulin(GLB) and γ-glutamyl transpeptidase (GGT) were measured and the fecal pH was recorded every day. The results showed that, ① when the pH of lactic acid treated water was 1~2, 2~3 and 3~4, the colony number of Escherichia coli and Salmonella was 0 at the beginning of 2 hours of culture. When the pH was 4~5, the number of colonies on the plate of the two strains decreased significantly after 8 hours of culture. When the pH was 5~6, the colony numbers of Escherichia coli and Salmonella decreased significantly at 24 h and 14 h respectively. ② TP and GLB of 13-day-old and 25-day-old Sanhuang chickens in the lactate drinking group were significantly higher than those in the control group (P<0.05); ALB was higher than that in the control group (P>0.05); GGT and A/G were not significantly different (P>0.05). Compared with the control group, there was no significant difference in fecal pH (P> 0.05), but there was a downward trend. It could be seen that when the pH of the lactic acid treated water was 1~4, it could effectively inhibit Escherichia coli and Salmonella in vitro. Drinking the lactic acid treated water with pH 3~4 was beneficial to the production of TP,ALB and GLB,which could promote the health of chickens, enhance the immunity of the body, and adjust the pH of the digestive tract.

Key words: lactic acid, antibacterial effect in vitro, Sanhuang chicken, serum index, fecal pH

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