湖北农业科学 ›› 2022, Vol. 61 ›› Issue (18): 169-172.doi: 10.14088/j.cnki.issn0439-8114.2022.18.029

• 贮藏·加工 • 上一篇    下一篇

青稞酒糟中蛋白质的提取工艺

樊世萌1, 刘浩1, 欧阳敬禹1,2, 刘恋1, 尤祥宇1,2, 苏江涛1,2, 肖红卫3   

  1. 1.湖北工业大学生物工程与食品学院,武汉 430068;
    2.襄阳湖北工业大学产业研究院,湖北 襄阳 441100;
    3.湖北省农业科学院畜牧兽医研究所动物胚胎工程与分子育种湖北省重点实验室,武汉 430072
  • 收稿日期:2022-03-15 出版日期:2022-09-25 发布日期:2022-10-21
  • 通讯作者: 尤祥宇(1983-),男,福建南安人,讲师,博士,主要从事功能高分子材料研究,(电话)17771822060(电子信箱)youxiangyu@hbut.edu.cn。
  • 作者简介:樊世萌(1997-),女(土家族),湖北恩施人,在读硕士研究生,研究方向为功能高分子材料,(电话)17671196825(电子信箱)770965319@qq.com。
  • 基金资助:
    动物胚胎工程及分子育种湖北省重点实验室开放课题(KLAEMB-2019-02); 襄阳湖北工业大学产业研究院2022年度项目(XYYJ2022C13)

Extraction technology of protein from highland barley distiller's grains

FAN Shi-meng1, LIU Hao1, OUYANG Jing-yu1,2, LIU Lian1, YOU Xiang-yu1,2, SU Jiang-tao1,2, XIAO Hong-wei3   

  1. 1. School of Biological Engineering and Food, Hubei University of Technology, Wuhan 430068, China;
    2. Xiangyang Industrial Institute of Hubei University of Technology, Xiangyang 441100, Hubei, China;
    3. Hubei Provincial Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan 430072, China
  • Received:2022-03-15 Online:2022-09-25 Published:2022-10-21

摘要: 采用醇碱溶、酸沉析的方法对青稞酒糟蛋白质进行提取。在单因素试验的基础上,选取浸提液醇碱比、固液比、浸提时间和浸提温度为影响因子,采用4因素3水平的正交试验,以蛋白质提取率作为指标,确定最佳提取工艺条件为浸提液醇碱比1∶3,固液比1∶45,浸提温度30 ℃,浸提时间90 min,各因素对蛋白质提取率的影响强弱顺序为固液比>醇碱比>浸提温度>浸提时间。

关键词: 青稞酒糟, 蛋白质提取, 正交试验

Abstract: The protein of highland barley distiller's grains was extracted by alcohol-alkali dissolution and acid precipitation. Based on the single factor experiment, the alcohol-alkali ratio, solid-liquid ratio, extraction time and extraction temperature were selected as the influencing factors. Using the orthogonal experiment of four factors and three levels, and taking the protein extraction rate as the index, the optimal extraction process condition was alcohol-alkali ratio of 1∶3, solid-liquid ratio of 1∶45, extraction temperature of 30 ℃, and extraction time of 90 min. The order of the influence of the various factors on the protein extraction rate was solid-liquid ratio > alcohol-alkali ratio > extraction temperature > extraction time.

Key words: highland barley distiller's grains, protein extraction, orthogonal test

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