昌江石斛内生抗菌活性菌株筛选与鉴定

doi:10.14088/j.cnki.issn0439-8114.2022.15.021

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (15): 124-128.doi: 10.14088/j.cnki.issn0439-8114.2022.15.021

昌江石斛内生抗菌活性菌株筛选与鉴定

高芳美^a, 陈夏诗^a, 陈茂楠^a, 高芳建^b, 杨文^c

海南医学院,a.第一临床学院;
b.第二临床学院;
c.基础医学与生命科学院,海口 571199

收稿日期:2021-09-03 出版日期:2022-08-10 发布日期:2022-08-25
通讯作者: 杨文（1973-）,女,高级试验师,硕士,主要从事抗感染免疫研究,（电子信箱）513108069@qq.com。
作者简介:高芳美（1998-）,女,海南东方人,在读本科生,专业方向为临床医学,（电话）13278952635（电子信箱）373453148@qq.com
基金资助:
海南医学院2019年大学生创新创业训练计划项目（x201911810033）

Screening and identification of endophytic antimicrobial strains from Dendrobium changjiangense

GAO Fang-mei^a, CHEN Xia-shi^a, CHEN Mao-nan^a, GAO Fang-jian^b, YANG Wen^c

a. First Clinical College;
b. Second Clinical College;
c. College of Basic Medicine and Life Sciences, Hainan Medical University, Haikou 571199, China

Received:2021-09-03 Online:2022-08-10 Published:2022-08-25

摘要/Abstract

摘要： 采用组织块分离法和划线分离法,从昌江石斛（Dendrobium changjiangense）植株体内分离得到内生细菌10株,以大肠埃希菌（Escherichia coli）、金黄色葡萄球菌（Staphylococcus aureus）和白假丝酵母菌（Candida albicans） 3株供试指示菌,采用琼脂平板法对分离的昌江石斛内生细菌进行抗菌活性的研究。结果表明,昌江石斛分离到的内生细菌中根部最多,茎部次之,叶部最少,昌江石斛所分离出的10株内生细菌中,有2株对至少1种供试菌有一定的抑菌作用,占总分离菌株的20%,其中C-06菌株对3种指示菌均具有抑菌作用,有1株细菌 C-05分别对2种指示菌有抑菌作用。通过细菌16S rRNA基因序列分析,活性菌株分布在芽孢杆菌属、类芽孢杆菌属。昌江石斛含有丰富的内生菌资源,部分内生菌具有代谢抑菌的特性,可作为新型药物的筛选源,为临床疾病的治疗提供可参考的途径。

关键词: 昌江石斛（Dendrobium changjiangense）, 内生菌, 抗菌活性, 菌属鉴定

Abstract: Ten endophytic bacteria were isolated from Dendrobium changjiangense by tissue block method and streak method, and three indicator bacteria including Escherichia coli, Staphylococcus aureus and Candida albicans were used to study the antimicrobial activity of endophytic bacteria isolated from Dendrobium changjiangense by agar plate method. The results showed that among the 10 endophytic bacteria isolated from Dendrobium changjiangensis, the root was the most, followed by the stem, and the leaf was the least. Among the 10 endophytic bacteria isolated from Dendrobium changjiangensis, two strains had inhibitory effects on at least one test bacterium, accounting for 20% of the total isolated strains. Among them, C-06 strain had inhibitory effects on the three indicator bacteria, and one strain C-05 had inhibitory effects on the two indicator bacteria, respectively. Through the analysis of bacterial 16S rRNA gene sequence, the active strains were distributed in Bacillus and Bacillus-like. Dendrobium changjiangense is rich in endophytic bacteria resources. Some endophytic bacteria have the characteristics of metabolic inhibition, which can be used as a screening source of new drugs and provide a reference for the treatment of clinical diseases.

Key words: Dendrobium chanjiangensis, endophytic bacteria, antimicrobial activity, genus identification

中图分类号:

Q946

高芳美, 陈夏诗, 陈茂楠, 高芳建, 杨文. 昌江石斛内生抗菌活性菌株筛选与鉴定[J]. 湖北农业科学, 2022, 61(15): 124-128.

GAO Fang-mei, CHEN Xia-shi, CHEN Mao-nan, GAO Fang-jian, YANG Wen. Screening and identification of endophytic antimicrobial strains from Dendrobium changjiangense[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 124-128.

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昌江石斛内生抗菌活性菌株筛选与鉴定

Screening and identification of endophytic antimicrobial strains from Dendrobium changjiangense

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