关键词: 川芎-葛根药对, 挥发性成分, 顶空固相微萃取, 气质联用分析

Abstract: The composition and pharmacological effects of volatile components in Chuanxiong-Gegen pair and single herb were analyzed. The volatile components in Chuanxiong-Gegen pair and single herb were qualitatively and quantitatively analyzed by solid phase microextraction-gas chromatography-mass spectrometry （SPME-GC-MS）, and the relative contents of each component were calculated by peak area normalization method. The result was a preliminary identification of 68 compounds. The highest volatile components（relative content） were β-methylphenylpropanal （46.84%）, m-cresol （48.49%） and （-）-α-solanolene （13.85%）. After the composition of drug pairs, 21 new components were added, and 32 original components were reduced. It was proved that this method was stable and reliable, and it was suitable for the rapid analysis of volatile components of Chuanxiong-Gegen pair and its single herb, which could provide a scientific basis for the clinical application of Chuanxiong and Gegen. The combined analysis of volatile components in Chuanxiong-Gegen pair and single herb could obtain more comprehensive information. The newly added components could guide the subsequent research on Chuanxiong-Gegen pair and its compound preparations, and the components in Chuanxiong-Gegen pair changed significantly before and after compatibility.

Key words: Chuanxiong-Gegen drug pair, volatile components, headspace solid phase microextraction, GC/MS analysis