湖北农业科学 ›› 2022, Vol. 61 ›› Issue (10): 99-105.doi: 10.14088/j.cnki.issn0439-8114.2022.10.017

• 园艺·特产 • 上一篇    下一篇

紫薇与大花紫薇种间杂种子代倍性分析

李振芳1, 彭婵1, 黄国伟1, 马林江1, 柯尊发2, 杨彦伶1   

  1. 1.湖北省林业科学研究院,武汉 430075;
    2.湖北省太子山林场管理局,湖北 京山 431822
  • 收稿日期:2021-03-03 出版日期:2022-05-25 发布日期:2022-06-14
  • 通讯作者: 杨彦伶(1962-),女,湖北武汉人,研究员,主要从事林木育种研究,(电话)13628621485(电子信箱)1974697950@qq.com。
  • 作者简介:李振芳(1982-),女,湖北潜江人,副研究员,硕士,主要从事林木育种研究,(电话)13986154648(电子信箱)lizhenfang82@qq.com。
  • 基金资助:
    湖北省知识创新专项(自然科学基金一般面上)资助项目(2018CFB703)

Analysis on the ploidy of hybrid seeds between Lagerstroemia indica and Lagerstroemia speciosa

LI Zhen-fang1, PENG Chan1, HUANG Guo-wei1, MA Lin-jiang1, KE Zun-fa2, YANG Yan-ling1   

  1. 1. Hubei Academy of Forestry,Wuhan 430075,China;
    2. Taizi Mountain Forest Management Bureau, Jingshan 431822,Hubei,China
  • Received:2021-03-03 Online:2022-05-25 Published:2022-06-14

摘要: 以尾叶紫薇为对照,用流式细胞仪测定屋久岛紫薇、福建紫薇、大花紫薇、紫薇品种Dynamite和12株紫薇(Lagerstroemia indica)×大花紫薇(Lagerstroemia speciosa)种间杂种子代植株均为二倍体;利用8条SSR标记判定Dynamite、大花紫薇、福建紫薇和26株紫薇×大花紫薇种间杂种子代植株的倍性均为二倍体,证实了流式细胞仪和SSR标记反映植物倍性的可行性。

关键词: 紫薇(Lagerstroemia indica), 大花紫薇(Lagerstroemia speciosa), 种间杂交, 流式细胞仪, SSR, 倍性

Abstract: Using Lagerstroemia caudata as comparison, L.fauriei, L.limii,L. speciosa, L. indica ‘Dynamite’ and 12 interspecific hybrids between L. indica and L. speciosa were diploid by detecting with flow cytometry. Eight SSR markers were used to determine the ploidy of Dynamite, L. speciosa, L. limii and 26 interspecific hybrids between L. indica and L. speciosa, and these plants were diploid. These results confirmed the feasibility of flow cytometry and SSR markers to reflect plant ploidy.

Key words: Lagerstroemia indica, Lagerstroemia speciosa, interspecific hybridization, flow cytometry, SSR, ploidy

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